中 / EN
Back
QUICK ORDER
Technical Support
| Contact Us
+1 312-802-6843
Technical Support
Contact Us
+1 312-802-6843
中 / EN
QUICK ORDER
Proteomics Bioinformation Research Antibody Products IVD Products Custom Antibody Development
Recover password
Professional Email
Verification Code
Send Code
Password
Recombinant Rabbit Monoclonal
Anti-L-Lactyl-Histone H3 (Lys18) Rabbit mAb
Catalog Number: PTM-1406RM
$ 420

Clone Number: 22H2L3

Host: Rabbit Clonality: Recombinant Monoclonal

Applications： WB IHC-P ICC/IF IP

Reactivity: Human, Mouse, Rat

Synonyms: H3K18la

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms H3K18la
UniProt ID

P68431

Immunogen L-lactylated human histone H3 (Lys18) peptide
MW (kDa) 15
Specificity Anti-L-Lactyl-Histone H3 (Lys18) Rabbit mAb detects histone H3 only when it is lactylated at Lys18.
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:2000 Human, Mouse, Rat
IHC-P 1:200 - 1:1000 Human
ICC/IF 1:50 - 1:100 Human
IP 1:50 Human
Properties
Purity Protein A purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Histones, fundamental proteins involved in chromatin structure and gene regulation, are subject to a wide array of enzyme-catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitination, and numerous others. Histone L-lactylation, a recently discovered post-translational modification induced by lactate has emerged as a significant addition to this repertoire. The extent and dynamics of this modification are highly reliant on lactate levels within the cellular microenvironment and can be modulated through the introduction of extracellular lactate in cultured cells or the stimulation of intracellular glycolysis. The introduction of lysine L-lactylation is mediated by the acetyltransferase p300, while the removal of lactylation marks from histones has been attributed to Class I histone deacetylases (HDAC 1-3). Histone lactylation has been implicated in various biological processes, including inflammation, fibrosis, differentiation, and cancer progression.

Cellular location

Nucleus

Images
Dot Blot

Peptide amount: 1 ng, 4 ng, 16 ng
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 60 seconds
The list of peptides used in the experiment is provided below.
Lane 1: H3K18 L-lactyl. Lane 2: H3K14 crotonyl.
Lane 3: H3K14 benzoyl. Lane 4: H3K14 L-lactyl.
Lane 5: H3K18 butyryl. Lane 6: H3K18 crotonyl.
Lane 7: H3K18 2-hydroxyisobutyryl. Lane 8: H3K18 benzoyl.
Lane 9: H3K23 crotonyl. Lane 10: H3K23 benzoyl.
Lane 11: H3K23 L-lactyl. Lane 12: H3K27 crotonyl.
Lane 13: H3K27 L-lactyl. Lane 14: H3K18 unmodified.
WB

Lysates: (-): HeLa cells; (+): HeLa cells treated with 100 mM sodium lactate for 24 hours
Protein loading amount: 20 μg
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 60 seconds
Predicted band size: 15 kDa
Observed band size: 15 kDa

Lysates: C2C12, RAW264.7, EL4.IL-2
Protein loading amount: 20 μg
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 60 seconds
Predicted band size: 15 kDa
Observed band size: 15 kDa

Lysates: PC-12, Rat spleen, Rat brain
Protein loading amount: 20 μg
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 60 seconds
Predicted band size: 15 kDa
Observed band size: 15 kDa
IHC-P

Tissue: Human breast cancer
Section type: Formalin fixed & paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (blue)
Description: The brown color represents the positive signal observed with PTM-1406RM.
ICC/IF

Samples: (A) HeLa cells; (B) HeLa cells treated with 100 mM sodium lactate for 24 hours
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% Triton X-100
Primary Ab dilution: 1:100
Primary Ab incubation condition: 4°C overnight
Secondary Ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (blue)
Counter stain: Tubulin (red)
Description: The green color represents the positive signal observed with PTM-1406RM.
IP

IP of HeLa+ sodium lactate (100 mM, 24 hours) extracts
Lane 1: 5% Input
Lane 2: IP with PTM-1406RM
Lane 3: IP with Rabbit mAb IgG Isotype Control
IP Ab incubation condition: 4°C overnight, 1:50 dilution
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
WB Primary Ab incubation condition: PTM-1406RM, 2 hours at room temperature, 1:1000 dilution
Secondary Ab: Anti-Rabbit IgG for IP (HRP Conjugate)
Exposure time: 30 seconds
Predicted band size: 15 kDa
Observed band size: 15 kDa

Research Use

For research use only, not for use in diagnostic procedures.

Recommended Products
Alternative Products Compatible Secondaries Isotype Control Writers/Readers/Erasers Related Products
  • View More Images（6）
    $ 435
    Recombinant Rabbit Monoclonal
    Anti-L-Lactyl-Histone H3 (Lys18) Rabbit mAb (ChIP Grade)
    Catalog #
    PTM-1427RM
    Host
    Rabbit
    Clonality
    Recombinant Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Mouse
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
  • View More Images（4）
    $ 135
    Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
    Catalog #
    PTM-6261
    Host
    Goat
    Clonality
    Polyclonal
    Conjugate
    HRP
    Reactivity
    Rabbit
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
  • View More Images（6）
    $ 290
    Recombinant Rabbit Monoclonal
    Anti-HDAC1 Rabbit mAb
    Catalog #
    PTM-6409
    Host
    Rabbit
    Clonality
    Recombinant Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Mouse, Rat
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
  • View More Images（1）
    $ 280
    Recombinant
    Anti-HDAC2 Mouse mAb
    Catalog #
    PTM-7219
    Host
    Mouse
    Clonality
    Recombinant Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Mouse, Rat
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
  • View More Images（3）
    $ 280
    Recombinant
    Anti-HDAC3 Mouse mAb
    Catalog #
    PTM-6070
    Host
    Mouse
    Clonality
    Recombinant Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Mouse, Rat
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
  • View More Images（12）
    $ 420
    Recombinant Rabbit Monoclonal
    Anti-L-Lactyllysine Rabbit mAb
    Catalog #
    PTM-1401RM
    Host
    Rabbit
    Clonality
    Recombinant Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    All
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
  • View More Images（1）
    $ 3,940
    Anti-L-Lactyllysine Antibody Conjugated Agarose Beads
    Catalog #
    PTM-1404
    Host
    Rabbit
    Clonality
    /
    Conjugate
    Agarose
    Reactivity
    All
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
  • View More Images（6）
    $ 435
    Recombinant Rabbit Monoclonal
    Anti-L-Lactyl-Histone H3 (Lys9) Rabbit mAb
    Catalog #
    PTM-1419RM
    Host
    Rabbit
    Clonality
    Recombinant Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Mouse, Rat
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
  • View More Images（7）
    $ 320
    Recombinant Rabbit Monoclonal
    Anti-Acetyl-Histone H3 (Lys18) Rabbit mAb
    Catalog #
    PTM-114RM
    Host
    Rabbit
    Clonality
    Recombinant Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Mouse, Rat
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
  • View More Images（2）
    $ 420
    Anti-β-Hydroxybutyryl-Histone H3 (Lys18) Mouse mAb
    Catalog #
    PTM-1292
    Host
    Mouse
    Clonality
    Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Mouse, Rat
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
  • View More Images（2）
    $ 435
    Recombinant
    Anti-Methacryl-Histone H3 (Lys18) Mouse mAb
    Catalog #
    PTM-1503
    Host
    Mouse
    Clonality
    Recombinant Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    Human
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
  • View More Images（2）
    $ 420
    Anti-Propionyl-Histone H3 (Lys18) Mouse mAb
    Catalog #
    PTM-214
    Host
    Mouse
    Clonality
    Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Mouse, Rat
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
  • View More Images（2）
    $ 420
    Recombinant Rabbit Monoclonal
    Anti-Butyryl-Histone H3 (Lys18) Rabbit mAb
    Catalog #
    PTM-306RM
    Host
    Rabbit
    Clonality
    Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    Human
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
References (12)
  • Hexokinase 2-mediated gene expression via histone lactylation is required for hepatic stellate cell activation and liver fibrosis
    Year 2023
    Journal Cell Metabolism
    Authors Hyunsoo Rho, et al.
    Applications Unspecified
    Reactivity
  • The diapause-like colorectal cancer cells induced by SMC4 attenuation are characterized by low proliferation and chemotherapy insensitivity
    Year 2023
    Journal Cell Metabolism
    Authors Xuedan Sun, et al.
    Applications Unspecified
    Reactivity
  • Positive feedback regulation of microglial glucose metabolism by histone H4 lysine 12 lactylation in Alzheimer’s disease
    Year 2022
    Journal Cell Metabolism
    Authors Rui-Yuan Pan, et al.
    Applications Unspecified
    Reactivity
  • Lactylation-driven METTL3-mediated RNA m6A modification promotes immunosuppression of tumor-infiltrating myeloid cells
    Year 2022
    Journal MOLECULAR CELL
    Authors Jia Xiong, et al.
    Applications Unspecified
    Reactivity
  • Histone lactylation driven by mROS-mediated glycolytic shift promotes hypoxic pulmonary hypertension
    Year 2022
    Journal Journal of Molecular Cell Biology
    Authors Chen Jian, et al.
    Applications Unspecified
    Reactivity
  • Altered phenotypic and metabolic characteristics of FOXP3+CD3+CD56+ natural killer T (NKT)-like cells in human malignant pleural effusion
    Year 2022
    Journal OncoImmunology
    Authors Zi-Hao Wang, et al.
    Applications Unspecified
    Reactivity
  • Lactate Activates Germline and Cleavage Embryo Genes in Mouse Embryonic Stem Cells
    Year 2022
    Journal Cells
    Authors Qing Tian, et al.
    Applications Unspecified
    Reactivity
  • Overexpression of Tfap2a in Mouse Oocytes Impaired Spindle and Chromosome Organization
    Year 2022
    Journal INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
    Authors Juan Lin, et al.
    Applications Unspecified
    Reactivity
  • Lactate Rewrites the Metabolic Reprogramming of Uveal Melanoma Cells and Induces Quiescence Phenotype
    Year 2022
    Journal INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
    Authors Lucia Longhitano, et al.
    Applications Unspecified
    Reactivity
  • H3K18 lactylation marks tissue-specific active enhancers
    Year 2022
    Journal GENOME BIOLOGY
    Authors Galle Eva, et al.
    Applications WB
    Reactivity
  • Comprehensive Analysis of Lysine Lactylation in Rice (Oryza sativa) Grains
    Year 2021
    Journal JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
    Authors Xiaoxi Meng, et al.
    Applications Unspecified
    Reactivity
  • Hypoxic in vitro culture reduces histone lactylation and impairs pre-implantation embryonic development in mice
    Year 2021
    Journal Epigenetics & Chromatin
    Authors Yang Wanting, et al.
    Applications Unspecified
    Reactivity
Service
Products
Support
About Us
© 2023 PTM Biolabs Inc.