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Recombinant Rabbit Monoclonal
Anti-L-Lactyl-Histone H3 (Lys18) Rabbit mAb
Catalog Number: PTM-1406RM
$ 420

Clone Number: 22H2L3

Host: Rabbit Clonality: Recombinant Monoclonal

Applications： WB IHC-P ICC/IF IP

Reactivity: Human, Mouse, Rat

Synonyms: H3K18la

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms H3K18la
UniProt ID

P68431

Immunogen L-lactylated human histone H3 (Lys18) peptide
MW (kDa) 15
Specificity Anti-L-Lactyl-Histone H3 (Lys18) Rabbit mAb detects histone H3 only when it is lactylated at Lys18.
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:2000 Human, Mouse, Rat
IHC-P 1:200 - 1:1000 Human
ICC/IF 1:50 - 1:100 Human
IP 1:50 Human
Properties
Purity Protein A purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Histones, fundamental proteins involved in chromatin structure and gene regulation, are subject to a wide array of enzyme-catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitination, and numerous others. Histone L-lactylation, a recently discovered post-translational modification induced by lactate has emerged as a significant addition to this repertoire. The extent and dynamics of this modification are highly reliant on lactate levels within the cellular microenvironment and can be modulated through the introduction of extracellular lactate in cultured cells or the stimulation of intracellular glycolysis. The introduction of lysine L-lactylation is mediated by the acetyltransferase p300, while the removal of lactylation marks from histones has been attributed to Class I histone deacetylases (HDAC 1-3). Histone lactylation has been implicated in various biological processes, including inflammation, fibrosis, differentiation, and cancer progression.

Cellular location

Nucleus

Images
Dot Blot

Peptide amount: 1 ng, 4 ng, 16 ng
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 60 seconds
The list of peptides used in the experiment is provided below.
Lane 1: H3K18 L-lactyl. Lane 2: H3K14 crotonyl.
Lane 3: H3K14 benzoyl. Lane 4: H3K14 L-lactyl.
Lane 5: H3K18 butyryl. Lane 6: H3K18 crotonyl.
Lane 7: H3K18 2-hydroxyisobutyryl. Lane 8: H3K18 benzoyl.
Lane 9: H3K23 crotonyl. Lane 10: H3K23 benzoyl.
Lane 11: H3K23 L-lactyl. Lane 12: H3K27 crotonyl.
Lane 13: H3K27 L-lactyl. Lane 14: H3K18 unmodified.
WB

Lysates: (-): HeLa cells; (+): HeLa cells treated with 100 mM sodium lactate for 24 hours
Protein loading amount: 20 μg
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 60 seconds
Predicted band size: 15 kDa
Observed band size: 15 kDa

Lysates: C2C12, RAW264.7, EL4.IL-2
Protein loading amount: 20 μg
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 60 seconds
Predicted band size: 15 kDa
Observed band size: 15 kDa

Lysates: PC-12, Rat spleen, Rat brain
Protein loading amount: 20 μg
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 60 seconds
Predicted band size: 15 kDa
Observed band size: 15 kDa
IHC-P

Tissue: Human breast cancer
Section type: Formalin fixed & paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (blue)
Description: The brown color represents the positive signal observed with PTM-1406RM.
ICC/IF

Samples: (A) HeLa cells; (B) HeLa cells treated with 100 mM sodium lactate for 24 hours
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% Triton X-100
Primary Ab dilution: 1:100
Primary Ab incubation condition: 4°C overnight
Secondary Ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (blue)
Counter stain: Tubulin (red)
Description: The green color represents the positive signal observed with PTM-1406RM.
IP

IP of HeLa+ sodium lactate (100 mM, 24 hours) extracts
Lane 1: 5% Input
Lane 2: IP with PTM-1406RM
Lane 3: IP with Rabbit mAb IgG Isotype Control
IP Ab incubation condition: 4°C overnight, 1:50 dilution
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
WB Primary Ab incubation condition: PTM-1406RM, 2 hours at room temperature, 1:1000 dilution
Secondary Ab: Anti-Rabbit IgG for IP (HRP Conjugate)
Exposure time: 30 seconds
Predicted band size: 15 kDa
Observed band size: 15 kDa

Research Use

For research use only, not for use in diagnostic procedures.

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References (51)
  • Lactylation-driven TNFR2 expression in regulatory T cells promotes the progression of malignant pleural effusion
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    Journal Journal for ImmunoTherapy of Cancer
    Authors Qianqian Xue, et al.
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  • Histone lactylation-driven YTHDC1 promotes hepatocellular carcinoma progression via lipid metabolism remodeling
    Year 2024
    Journal CANCER LETTERS
    Authors Wenfei Du, et al.
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  • Tanshinone I reprograms glycolysis metabolism to regulate histone H3 lysine 18 lactylation (H3K18la) and inhibits cancer cell growth in ovarian cancer
    Year 2024
    Journal INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
    Authors Zhou Jin, et al.
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  • Histone Lactylation-Driven GPD2 Mediates M2 Macrophage Polarization to Promote Malignant Transformation of Cervical Cancer Progression
    Year 2024
    Journal DNA AND CELL BIOLOGY
    Authors Chenlingzi Huang, et al.
    Applications Unspecified
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  • Nuclear GTPSCS functions as a lactyl-CoA synthetase to promote histone lactylation and gliomagenesis
    Year 2024
    Journal Cell Metabolism
    Authors Ruilong Liu, et al.
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  • Dual activation of GCGR/GLP1R signaling ameliorates intestinal fibrosis via metabolic regulation of histone H3K9 lactylation in epithelial cells
    Year 2024
    Journal Acta Pharmaceutica Sinica B
    Authors Han Liu, et al.
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  • Histone lactylation mediated by Fam172a in POMC neurons regulates energy balance
    Year 2024
    Journal Nature Communications
    Authors Chen Zhuo, et al.
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  • GLP-1R activation attenuates the progression of pulmonary fibrosis via disrupting NLRP3 inflammasome/PFKFB3-driven glycolysis interaction and histone lactylation
    Year 2024
    Journal Journal of Translational Medicine
    Authors Liu Chenyang, et al.
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  • Pre-implantation embryo metabolism identified by PEMA reveals endogenous lactate insufficiency contributes to pre-implantation development arrest
    Year 2024
    Journal Fundamental Research
    Authors Jingyu Li, et al.
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  • Lactylation drives hCG-triggered luteinization in hypoxic granulosa cells
    Year 2024
    Journal INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
    Authors Gang Wu, et al.
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  • Sirtuin 1/sirtuin 3 are robust lysine delactylases and sirtuin 1-mediated delactylation regulates glycolysis
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    Authors Hu Xuyang, et al.
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  • Gegen Qinlian decoction inhibited M1 macrophage polarization and ulcerative colitis progression through regulating histone lactylation
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    Journal TISSUE & CELL
    Authors Zhen-Peng Xu, et al.
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    Authors Wei Yaozong, et al.
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  • ChIP-seq and RNA-seq Reveal the Involvement of Histone Lactylation Modification in Gestational Diabetes Mellitus
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    Authors Xiaman Huang, et al.
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  • Histone H3K18 and Ezrin Lactylation Promote Renal Dysfunction in Sepsis-Associated Acute Kidney Injury
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    Authors Jiao Qiao, et al.
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  • Macrophage MCT4 inhibition activates reparative genes and protects from atherosclerosis by histone H3 lysine 18 lactylation
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    Authors Yunjia Zhang, et al.
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  • Highly expressed lncRNA H19 in endometriosis promotes aerobic glycolysis and Histone lactylation
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    Applications Unspecified
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    Authors Morgan Towriss, et al.
    Applications Unspecified
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    Authors Wei Lin, et al.
    Applications Unspecified
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    Journal Molecular Cancer
    Authors Xie Bo, et al.
    Applications Unspecified
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    Authors Ying Yu, et al.
    Applications Unspecified
    Reactivity
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    Authors Chaudagar Kiranj, et al.
    Applications Unspecified
    Reactivity
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    Year 2022
    Journal Cell Metabolism
    Authors Rui-Yuan Pan, et al.
    Applications Unspecified
    Reactivity
  • Lactylation-driven METTL3-mediated RNA m6A modification promotes immunosuppression of tumor-infiltrating myeloid cells
    Year 2022
    Journal MOLECULAR CELL
    Authors Jia Xiong, et al.
    Applications Unspecified
    Reactivity
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    Year 2022
    Journal Journal of Molecular Cell Biology
    Authors Chen Jian, et al.
    Applications Unspecified
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  • Altered phenotypic and metabolic characteristics of FOXP3+CD3+CD56+ natural killer T (NKT)-like cells in human malignant pleural effusion
    Year 2022
    Journal OncoImmunology
    Authors Zi-Hao Wang, et al.
    Applications Unspecified
    Reactivity
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    Year 2022
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    Authors Qing Tian, et al.
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    Year 2022
    Journal INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
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    Reactivity
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    Year 2022
    Journal INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
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    Reactivity
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    Year 2022
    Journal GENOME BIOLOGY
    Authors Galle Eva, et al.
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  • Comprehensive Analysis of Lysine Lactylation in Rice (Oryza sativa) Grains
    Year 2021
    Journal JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
    Authors Xiaoxi Meng, et al.
    Applications Unspecified
    Reactivity
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    Year 2021
    Journal Epigenetics & Chromatin
    Authors Yang Wanting, et al.
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