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Recombinant Rabbit Monoclonal
Anti-L-Lactyl-Histone H3 (Lys18) Rabbit pAb
Catalog Number: PTM-1406
References (28)
This item is discontinued.
Please see alternative products below.

Clone Number: /

Host: Rabbit Clonality: Polyclonal

Applications： WB FC ChIP

Reactivity: Human, Mouse, Rat

Synonyms: H3K18la

Product Size
100μl

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

Alternative Products
  • View More Images（7）
    $ 420
    Recombinant Rabbit Monoclonal
    Anti-L-Lactyl-Histone H3 (Lys18) Rabbit mAb
    Catalog #
    PTM-1406RM
    Host
    Rabbit
    Clonality
    Recombinant Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Mouse, Rat
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P CUT&Tag
  • View More Images（6）
    $ 435
    Recombinant Rabbit Monoclonal
    Anti-L-Lactyl-Histone H3 (Lys18) Rabbit mAb (ChIP Grade)
    Catalog #
    PTM-1427RM
    Host
    Rabbit
    Clonality
    Recombinant Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Mouse
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P CUT&Tag
General Information
Isotype IgG
Conjugate Unconjugated
Synonyms H3K18la
UniProt ID

P68431

Immunogen Lactylated human histone H3 (Lys18) peptide
MW (kDa) 15
Specificity Anti-Lactyl-Histone H3 (Lys18) Rabbit pAb detects histone H3 only when it is lactylated at Lys18. This antibody has been shown to selectively recognize lactylated H3 peptide at Lys18, but not the acetylated, crotonylated, butyrylated, propionylated, or unmodified H3 peptide at Lys18.
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:2000 Human, Mouse, Rat
FC 1:50 - 1:100 Human
ChIP 1:100 - 1:500 Human
Properties
Purity Protein A and immunogen affinity purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Histones are subject to a variety of enzyme-catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitination, etc. The Warburg effect, initially characterized by an increase in lactate production in cancer cells, is linked to various cellular processes such as angiogenesis, hypoxia, polarization of macrophages, and T cell activation. This phenomenon is closely associated with several diseases including neoplasia, sepsis and autoimmune diseases. Lactate, which is converted from pyruvate in tumor cells, is well-known as an energy source and metabolic byproduct. Zhang et al. (Nature, 2019) reveal that histone lactylation is a previously unknown histone modification derived from the cellular metabolite lactate.

Cellular location

Nucleus

Images
Dot Blot

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP)
Peptide amount: 1 ng, 4 ng, 16 ng
Exposure time: 60 s
The list of peptides is included in the table below.
https://s2.loli.net/2022/08/08/BQ34Cpz8EmTZisK.png
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: (-) HeLa; (+) HeLa + sodium lactate (100 mM, 24 h)
Protein loading amount: 20 μg
Exposure time: 60 s
Predicted band size: 15 kDa
Observed band size: 15 kDa

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: N2a, BRL
Protein loading amount: 20 μg
Exposure time: 60 s
Predicted band size: 15 kDa
Observed band size: 15 kDa
FC

Cell line: HeLa
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% Triton X-100
Primary Ab dilution: 1:100
Secondary Ab: Goat anti-Rabbit IgG
Unlabeled control: Cells without incubation with primary and secondary antibodies (black line).
Isotype control: Rabbit monoclonal IgG (blue line).
Comment: Line red is the positive signal for PTM-1406.
ChIP

Cell type: MCF-7
Cross-linking conditions: No cross-linking
Amount of chromatin per IP: 5x106 cells
Amount of Ab per IP: 1:500, 1:200
Beads type and amount per IP: 50 μl of Protein A/G MagBeads
Comment: The ChIP assay was performed with 1 μg of normal rabbit IgG as a negative control. The immunoprecipitated DNA was quantified by real-time PCR using primers specific for the human GAPDH-CDS region and RPL30 Exon 3. The data are presented as enrichment of each sample relative to total amount of input chromatin at each amplicon.

Research Use

For research use only, not for use in diagnostic procedures.

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References (28)
  • Microvascular endothelial metabolic dysfunction drives cerebral edema through bioenergetic failure after ischemia-reperfusion
    Year 2026
    Journal Theranostics
    Authors Yi-Fan Zhou, et al.
    Applications Unspecified
    Reactivity
  • Spatial heterogeneity of lactylation: Insights into gene expression, metabolism, and lactate transport in human embryonic stem cells
    Year 2025
    Journal Biology Open
    Authors Kozlov Alexandra M., et al.
    Applications Unspecified
    Reactivity
  • Hypoxia facilitates stemness of colon cancer cells via histone lactylation
    Year 2025
    Journal BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
    Authors Qi Zhang, et al.
    Applications Unspecified
    Reactivity
  • PFKM Promotes the Progression of Gastric Cancer by Up-regulating CNTN1 Expression Through H3K18la Modification
    Year 2025
    Journal APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
    Authors Shen Xin, et al.
    Applications Unspecified
    Reactivity
  • Long-term histone lactylation connects metabolic and epigenetic rewiring in innate immune memory
    Year 2025
    Journal CELL
    Authors Athanasios Ziogas, et al.
    Applications Unspecified
    Reactivity
  • Cold atmospheric plasma drives USP49/HDAC3 axis mediated ferroptosis as a novel therapeutic strategy in endometrial cancer via reinforcing lactylation dependent p53 expression
    Year 2025
    Journal Journal of Translational Medicine
    Authors Liu Jinlin, et al.
    Applications Unspecified
    Reactivity
  • Hypoxia-Inducible Factor-1α Regulates BNIP3-Dependent Mitophagy and Mediates Metabolic Reprogramming Through Histone Lysine Lactylation Modification to Affect Glioma Proliferation and Invasion
    Year 2025
    Journal JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY
    Authors Feng Dong, et al.
    Applications Unspecified
    Reactivity
  • Inhibition of PKM2 by shikonin impedes TGF-β1 expression by repressing histone lactylation to alleviate renal fibrosis
    Year 2024
    Journal PHYTOMEDICINE
    Authors Tianya Xiang, et al.
    Applications Unspecified
    Reactivity
  • Histone H4K12 lactylation promotes malignancy progression in triple-negative breast cancer through SLFN5 downregulation
    Year 2024
    Journal CELLULAR SIGNALLING
    Authors Jingyi Li, et al.
    Applications Unspecified
    Reactivity
  • Histone lactylation promotes malignant progression by facilitating USP39 expression to target PI3K/AKT/HIF-1α signal pathway in endometrial carcinoma
    Year 2024
    Journal Cell Death Discovery
    Authors Wei Sitian, et al.
    Applications Unspecified
    Reactivity
  • Augmentation of scleral glycolysis promotes myopia through histone lactylation
    Year 2024
    Journal Cell Metabolism
    Authors Xiaolei Lin, et al.
    Applications Unspecified
    Reactivity
  • Histone lactylation couples cellular metabolism with developmental gene regulatory networks
    Year 2024
    Journal Nature Communications
    Authors Merkuri Fjodor, et al.
    Applications Unspecified
    Reactivity
  • Hexokinase 2-mediated gene expression via histone lactylation is required for hepatic stellate cell activation and liver fibrosis
    Year 2023
    Journal Cell Metabolism
    Authors Hyunsoo Rho, et al.
    Applications Unspecified
    Reactivity
  • Porphyromonas gingivalis msRNA P.G_45033 induces amyloid-β production by enhancing glycolysis and histone lactylation in macrophages
    Year 2023
    Journal INTERNATIONAL IMMUNOPHARMACOLOGY
    Authors Yonghuan Zhang, et al.
    Applications Unspecified
    Reactivity
  • High-concentrate diet elevates histone lactylation mediated by p300/CBP through the upregulation of lactic acid and induces an inflammatory response in mammary gland of dairy cows
    Year 2023
    Journal MICROBIAL PATHOGENESIS
    Authors Lairong Wang, et al.
    Applications Unspecified
    Reactivity
  • Lactate dehydrogenase A mediated histone lactylation induced the pyroptosis through targeting HMGB1
    Year 2023
    Journal METABOLIC BRAIN DISEASE
    Authors Yao Xuan, et al.
    Applications Unspecified
    Reactivity
  • Lactate induces tumor-associated macrophage polarization independent of mitochondrial pyruvate carrier-mediated metabolism
    Year 2023
    Journal INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
    Authors Xia Fang, et al.
    Applications Unspecified
    Reactivity
  • Numb/Parkin-directed mitochondrial fitness governs cancer cell fate via metabolic regulation of histone lactylation.
    Year 2023
    Journal Cell Reports
    Authors He Y, et al.
    Applications Unspecified
    Reactivity
  • Class I histone deacetylases (HDAC1–3) are histone lysine delactylases
    Year 2022
    Journal Science Advances
    Authors Carlos Moreno-Yruela, et al.
    Applications Unspecified
    Reactivity
  • Hypoxia regulates fibrosis-related genes via histone lactylation in the placentas of patients with preeclampsia
    Year 2022
    Journal JOURNAL OF HYPERTENSION
    Authors Li Xiang, et al.
    Applications Unspecified
    Reactivity
  • Methylsulfonylmethane protects against lethal dose MRSA-induced sepsis through promoting M2 macrophage polarization
    Year 2022
    Journal MOLECULAR IMMUNOLOGY
    Authors Wei Ma, et al.
    Applications Unspecified
    Reactivity
  • LDHA promotes osteoblast differentiation through histone lactylation
    Year 2022
    Journal BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
    Authors Feige Nian, et al.
    Applications Unspecified
    Reactivity
  • H3K18 lactylation marks tissue-specific active enhancers
    Year 2022
    Journal GENOME BIOLOGY
    Authors Galle Eva, et al.
    Applications WB, CUT&Tag
    Reactivity
  • Histone lactylation drives oncogenesis by facilitating m6A reader protein YTHDF2 expression in ocular melanoma
    Year 2021
    Journal GENOME BIOLOGY
    Authors Yu Jie, et al.
    Applications Unspecified
    Reactivity
  • Lactic Acid-Producing Probiotic Saccharomyces cerevisiae Attenuates Ulcerative Colitis via Suppressing Macrophage Pyroptosis and Modulating Gut Microbiota.
    Year 2021
    Journal Frontiers in Immunology
    Authors Sun S, et al.
    Applications Unspecified
    Reactivity
  • A proteomic atlas of ligand-receptor interactions at the ovine maternal-fetal interface reveals the role of histone lactylation in uterine remodeling
    Year 2021
    Journal JOURNAL OF BIOLOGICAL CHEMISTRY
    Authors Qianying Yang, et al.
    Applications Unspecified
    Reactivity
  • Lactic Acid-Producing Probiotic Saccharomyces cerevisiae Attenuates Ulcerative Colitis via Suppressing Macrophage Pyroptosis and Modulating Gut Microbiota
    Year 2021
    Journal Frontiers in Immunology
    Authors Sun Siyuan, et al.
    Applications Unspecified
    Reactivity
  • Metabolic regulation of gene expression by histone lactylation
    Year 2019
    Journal NATURE
    Authors Zhang Di, et al.
    Applications Unspecified
    Reactivity
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