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Recombinant Rabbit Monoclonal
Anti-L-Lactyl-Histone H3 (Lys23) Rabbit mAb
Catalog Number: PTM-1413RM
References (14)
$ 435

Clone Number: PA-148(B)-213

Host: Rabbit Clonality: Recombinant Monoclonal

Applications： WB IHC-P ChIP

Reactivity: Human, Mouse, Rat

Synonyms: H3K23la

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms H3K23la
UniProt ID

P68431

Immunogen L-lactylated human histone H3 (Lys23) peptide
MW (kDa) 15
Specificity Anti-L-Lactyl-Histone H3 (Lys23) Rabbit mAb detects endogenous levels of histone H3 when it is L-lactylated at Lys23. This antibody shows minor cross-reactivity to L-lactyl-histone H3 (Lys18) peptide.
Product Usage Information
Applications Dilution Recommended Species
WB 1:2000 - 1:10000 Human, Mouse, Rat
IHC-P 1:200 - 1:1000 Human
ChIP 6 μg per 5x106 cells Human
Properties
Purity Protein A purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Histones, fundamental proteins involved in chromatin structure and gene regulation, are subject to a wide array of enzyme-catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitination, and numerous others. Histone L-lactylation, a recently discovered post-translational modification induced by lactate has emerged as a significant addition to this repertoire. The extent and dynamics of this modification are highly reliant on lactate levels within the cellular microenvironment and can be modulated through the introduction of extracellular lactate in cultured cells or the stimulation of intracellular glycolysis. The introduction of lysine L-lactylation is mediated by the acetyltransferase p300, while the removal of lactylation marks from histones has been attributed to Class I histone deacetylases (HDAC 1-3). Histone lactylation has been implicated in various biological processes, including inflammation, fibrosis, differentiation, and cancer progression.

Cellular location

Nucleus

Images
Dot Blot

Peptide amount: 1 ng, 4 ng, 16 ng, 64 ng
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 30 seconds
The list of peptides used in the experiment is provided below.
Lane 1: H3K23 L-lactyl. Lane 2: H3K18 L-lactyl.
Dot 3: H3K23 acetyl. Dot 4: H3K23 crotonyl.
Dot 5: H3K23 butyryl. Dot 6: H3K23 protonyl.
Dot 7: H3K23 unmodified.
WB

Lysates: (-) HeLa cells; (+) HeLa cells treated with 100 mM sodium lactate for 24 hours
Protein loading amount: 20 μg
Blocking buffer: 2.5% BSA/TBST
Primary Ab dilution: 1:10000 (2.5% BSA)
Primary Ab incubation: 4°C overnight
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 10 seconds
Predicted band size: 15 kDa
Observed band size: 15 kDa
Important Note: It is recommended to employ 2.5% BSA/TBST for blocking and antibody dilution. Using 5% nonfat milk may result in nonspecific bands.

Lysates: (-) NIH-3T3 cells; (+) NIH-3T3 cells treated with 25 mM sodium lactate for 24 hours
Protein loading amount: 20 μg
Blocking buffer: 2.5% BSA/TBST
Primary Ab dilution: 1:10000 (2.5% BSA)
Primary Ab incubation: 4°C overnight
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 10 seconds
Predicted band size: 15 kDa
Observed band size: 15 kDa
Important Note: It is recommended to employ 2.5% BSA/TBST for blocking and antibody dilution. Using 5% nonfat milk may result in nonspecific bands.

Lysates: (-) C6 cells; (+) C6 cells treated with 25 mM sodium lactate for 24 hours
Protein loading amount: 20 μg
Blocking buffer: 2.5% BSA/TBST
Primary Ab dilution: 1:10000 (2.5% BSA)
Primary Ab incubation: 4°C overnight
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 10 seconds
Predicted band size: 15 kDa
Observed band size: 15 kDa
Important Note: It is recommended to employ 2.5% BSA/TBST for blocking and antibody dilution. Using 5% nonfat milk may result in nonspecific bands.
IHC-P

Tissue: Human colon
Section type: Formalin fixed & paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (blue)
Description: The brown color represents the positive signal observed with PTM-1413RM.

Tissue: Human placenta
Section type: Formalin fixed & paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (blue)
Description: The brown color represents the positive signal observed with PTM-1413RM.
ChIP

Sample: HeLa cells treated with 25 mM sodium lactate for 24 hours
Cross-linking conditions: no cross-linking
Amount of chromatin per IP: 5×106 cells
Amount of Ab per IP: 6 μg
Beads type and amount per IP: 50 μL of Protein A MagBeads
Description: Chromatin immunoprecipitations were performed with 6 μg of normal rabbit IgG as a negative control. The immunoprecipitated DNA was quantified by real-time PCR using primers specific for the human RPL30, LDHA promoter, FOXO3a promoter, FOXO3a downstream, RAB20, and TUBBP10 regions. The data are presented as enrichment of each sample relative to the total amount of input at each amplicon.

Research Use

For research use only, not for use in diagnostic procedures.

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References (14)
  • Lactate derived from macrophages drives skin dermal fibroblasts phenotypic remodeling via MCT1-primed histone H3 lysine 23 lactylation in hypertrophic scar
    Year 2026
    Journal Nature Communications
    Authors Yuan Yixuan, et al.
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  • Histone lactylation dynamics are associated with impaired maternal-to-zygotic transition in vitro–cultured goat embryos
    Year 2026
    Journal THERIOGENOLOGY
    Authors Jinhao Zhang, et al.
    Applications Unspecified
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  • Coniferyl Aldehyde in Ginger-Eucommiae Cortex Enhances Osteoarthritis Treatment by Modulating ALDOA and H3K23la Histone Lactylation
    Year 2026
    Journal PHYTOMEDICINE
    Authors Yijing Pan, et al.
    Applications Unspecified
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  • Jaceosidin Attenuates Sepsis-Induced Myocardial Dysfunction by Promoting SIRT2-Mediated Inhibition of Histone H3K18 Lactylation
    Year 2026
    Journal Pharmaceuticals
    Authors Huiming Yu, et al.
    Applications Unspecified
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  • Geniposidic Acid from Ginger-processed Eucommiae Cortex Alleviates Rheumatoid Arthritis by Modulating Macrophage Lactylation Induced by Exosomes from Inflammatory Fibroblast-Like Synoviocytes
    Year 2025
    Journal JOURNAL OF NUTRITIONAL BIOCHEMISTRY
    Authors Yijing Pan, et al.
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  • Lactylated histone H4K8 regulation of MFN2/Wnt signaling integrates glycolytic metabolism and Müller cell activation in the pathogenesis of glaucoma
    Year 2025
    Journal NEUROBIOLOGY OF DISEASE
    Authors Shiyuan Xiao, et al.
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  • H3K18 lactylation potentiates microglial polarization via the TLR4 pathway in diabetes-induced cognitive impairment
    Year 2025
    Journal JCI Insight
    Authors Ying Yang, et al.
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  • Histone Lactylation-Mediated PAD4 Up-Regulation Promotes Septic Acute Kidney Injury via Activating NETosis
    Year 2025
    Journal NEPHROLOGY
    Authors Wen-lin Huang, et al.
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  • H3K18 lactylation-hexokinase 2 positive feedback loop promotes osteogenesis of ASPCs in facial infiltrating lipomatosis
    Year 2025
    Journal Stem Cell Research & Therapy
    Authors Chen Hongrui, et al.
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  • Chronic intermittent hypoxia impairs BM-MSC osteogenesis and long bone growth through regulating histone lactylation
    Year 2025
    Journal Journal of Translational Medicine
    Authors Chen Fang, et al.
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  • Luteolin attenuates RA-associated chronic pain by targeting the LDHA/H3K9la/NFATC2 axis to suppress Th17 cell differentiation and central infiltration
    Year 2025
    Journal Journal of Pharmaceutical Analysis
    Authors Yuepeng Jiang, et al.
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  • Gastrodin regulates H3K14la through the CDT2-KAT2A axis to treat Sepsis-induced myocardial dysfunction
    Year 2025
    Journal INTERNATIONAL IMMUNOPHARMACOLOGY
    Authors Huiming Yu, et al.
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  • Gastrodin attenuates rheumatoid arthritis by targeting KAT8 to inhibit the lactylation of H3K9
    Year 2025
    Journal Frontiers in Pharmacology
    Authors Yufang Dai, et al.
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  • Lactate drives epithelial-mesenchymal transition in diabetic kidney disease via the H3K14la/KLF5 pathway
    Year 2024
    Journal Redox Biology
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