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Recombinant Rabbit Monoclonal
Anti-L-Lactyl-Histone H4 (Lys16) Rabbit mAb
Catalog Number: PTM-1417RM
References (25)
$ 435

Clone Number: 2H2L1

Host: Rabbit Clonality: Recombinant Monoclonal

Applications： WB IHC-P ICC/IF ChIP

Reactivity: Human, Mouse, Rat

Synonyms: H4K16la

Product Size
100μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms H4K16la
UniProt ID

P62805

Immunogen L-lactylated human histone H4 (Lys16) peptide
MW (kDa) 11
Specificity Anti-L-Lactyl-Histone H4 (Lys16) Rabbit mAb detects histone H4 only when it is L-lactylated at Lys16.
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:2000 Human, Mouse, Rat
IHC-P 1:100 - 1:500 Human
ICC/IF 1:500 Human
ChIP 6 μg per 5x106 cells Human
Properties
Purity Protein A purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Histones, fundamental proteins involved in chromatin structure and gene regulation, are subject to a wide array of enzyme-catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitination, and numerous others. Histone L-lactylation is a recently discovered post-translational modification that is induced by lactate, a metabolite produced during glycolysis. Regulated by glucose metabolic dynamics and lactate levels within the cellular microenvironment, histone lactylation is implicated in various biological processes, including inflammation, fibrosis, differentiation, and cancer progression. The introduction of lysine L-lactylation is mediated by the acetyltransferase p300, while the removal of lactylation marks from histones has been attributed to Class I histone deacetylases (HDAC 1-3). Notably, Sirtuin 3 (SIRT3) has been reported as an eraser of H4K16 lactylation (H4K16la).

Cellular location

Nucleus

Images
Dot Blot

Peptide amount: 1 ng, 4 ng, 16 ng
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:10000
Primary Ab incubation: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 10 seconds
The list of peptides used in the experiment is provided below.
Lane 1: H4K16 L-lactyl. Lane 2: H4K12 L-lactyl.
Lane 3: H4K16 crotonyl. Lane 4: H4K16 2-hydroxyisobutyryl.
Lane 5: H4K16 unmodified.
WB

Lysates: (-) HeLa cells; (+) HeLa cells treated with 100 mM sodium lactate for 24 hours
Protein loading amount: 20 μg
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 60 seconds
Predicted band size: 11 kDa
Observed band size: 11 kDa
IHC-P

Tissue: Human colon cancer
Section type: Formalin fixed & paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:500
Primary Ab incubation: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (blue)
Description: The brown color represents the positive signal observed with PTM-1417RM.
ICC/IF

Samples: (A) HeLa cells; (B) HeLa cells treated with 100 mM sodium lactate for 24 hours
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% Triton X-100
Primary Ab dilution: 1:500
Primary Ab incubation: 4°C overnight
Secondary Ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (blue)
Counter stain: Tubulin (red)
Description: The green color represents the positive signal observed with PTM-1417RM.
ChIP

Sample: HeLa cells treated with 100 mM sodium lactate for 24 hours
Cross-linking conditions: no cross-linking
Amount of chromatin per IP: 5x106 cells
Amount of Ab per IP: 6 μg
Beads type and amount per IP: 50 μL of Protein A MagBeads
Description: Chromatin immunoprecipitations were performed with 6 μg of normal rabbit IgG as a negative control. The immunoprecipitated DNA was quantified by real-time PCR using primers specific for the human RPL30 exon 3 and TUBBP10 regions. The data are presented as enrichment of each sample relative to the total amount of input at each amplicon.

Research Use

For research use only, not for use in diagnostic procedures.

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References (25)
  • Histone H4 lysine 5 lactylation: A key regulator of immune metabolism in microglia during ischemic stroke
    Year 2026
    Journal Pharmacol Res
    Authors Yishan Li, et al.
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  • Histone H4K8 lactylation promotes glioblastoma progression by inducing NUPR1-mediated autophagosome‒lysosome fusion
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    Journal Theranostics
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  • Jaceosidin Attenuates Sepsis-Induced Myocardial Dysfunction by Promoting SIRT2-Mediated Inhibition of Histone H3K18 Lactylation
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    Journal Pharmaceuticals (Basel)
    Authors Huiming Yu, et al.
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  • Microvascular endothelial metabolic dysfunction drives cerebral edema through bioenergetic failure after ischemia-reperfusion
    Year 2026
    Journal Theranostics
    Authors Yi-Fan Zhou, et al.
    Applications WB
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  • STING controls glycolysis and histone lactylation to drive macrophage metabolic reprogramming in postoperative ileus
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    Journal Commun Biol
    Authors Kai Chen, et al.
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  • Targeting MYC in T-Cell lymphoma via epigenetic modulation with a small-molecule inhibitor
    Year 2026
    Journal Mol Cancer
    Authors Bangong Liu, et al.
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  • NPTX2 accelerates cell cycle progression, activates EMT, and regulates glycolytic metabolic pathways to promote clear cell renal cell carcinoma progression
    Year 2026
    Journal Biochem Pharmacol
    Authors Dong Li, et al.
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    Reactivity
  • Carnosol targets KAT2B/H3K18la axis to regulate histone 2 lactylation and inhibit proliferation of lung cancer cells
    Year 2026
    Journal SSRN
    Authors Minfu Liu, et al.
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  • Elevated lactate production exacerbates PM2.5-induced pulmonary fibrosis by stabilizing TGF-β1
    Year 2025
    Journal J Adv Res
    Authors Zhihao Liu, et al.
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  • Enhanced glycolysis-derived lactate promotes microglial activation in Parkinson’s disease via histone lactylation
    Year 2025
    Journal NPJ Parkinsons Dis
    Authors Qin Qixiong, et al.
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  • Gastrodin attenuates rheumatoid arthritis by targeting KAT8 to inhibit the lactylation of H3K9
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    Journal Front Pharmacol
    Authors Yufang Dai, et al.
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  • Gastrodin regulates H3K14la through the CDT2-KAT2A axis to treat Sepsis-induced myocardial dysfunction
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  • Hexokinase 2-mediated histone H3K18la promotes PAI-1-dependent thrombosis in acute myeloid leukemia via tumor-endothelial crosstalk
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  • Lactate promotes collagen expression, proliferation and migration through H3K18 lactylation-dependent stimulation of LTBP3/TGF-β1 axis in keloid fibroblasts
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  • Lactylated histone H4K8 regulation of MFN2/Wnt signaling integrates glycolytic metabolism and Müller cell activation in the pathogenesis of glaucoma
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  • Quantitative Proteomics Reveals the Role of Lysine Lactylation in Lenalidomide-Resistance in Multiple Myeloma Cells
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    Authors Wang Zhen-chuan, et al.
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  • TRPM7 Deficiency Accelerates Vascular Senescence by Inhibiting H3K18 Lactylation
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    Journal Aging Cell
    Authors Yue Wang, et al.
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  • WTAP Mediated m6A Modification Stabilizes PDIA3P1 and Promotes Tumor Progression Driven by Histone Lactylation in Esophageal Squamous Cell Carcinoma
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    Journal Adv Sci (Weinh)
    Authors Tao Huang, et al.
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  • Histone lactylation-driven B7-H3 expression promotes tumor immune evasion
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    Journal Theranostics
    Authors Zhibo Ma, et al.
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  • DNAJC12 downregulation induces neuroblastoma progression via increased histone H4K5 lactylation
    Year 2024
    Journal J Mol Cell Biol
    Authors Yang Yaqi, et al.
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  • Dual activation of GCGR/GLP1R signaling ameliorates intestinal fibrosis via metabolic regulation of histone H3K9 lactylation in epithelial cells
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  • Comprehensive Analysis of Lysine Lactylation in Rice (Oryza sativa) Grains
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