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Recombinant Rabbit Monoclonal
Anti-L-Lactyl-Histone H4 (Lys5) Rabbit mAb
Catalog Number: PTM-1407RM
$ 420

Clone Number: 17H1L1

Host: Rabbit Clonality: Recombinant Monoclonal

Applications： WB ICC/IF FC IP

Reactivity: Human, Mouse, Rat

Synonyms: H4K5la

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms H4K5la
UniProt ID

P62805

Immunogen L-lactylated human histone H4 (Lys5) peptide
MW (kDa) 11
Specificity Anti-L-Lactyl-Histone H4 (Lys5) Rabbit mAb detects histone H4 only when it is L-lactylated at Lys5.
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:2000 Human, Mouse, Rat
ICC/IF 1:100 Human
FC 1:50 - 1:100 Human
IP 1:50 Human
Properties
Purity Protein A purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Histones, fundamental proteins involved in chromatin structure and gene regulation, are subject to a wide array of enzyme-catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitination, and numerous others. Histone L-lactylation, a recently discovered post-translational modification induced by lactate has emerged as a significant addition to this repertoire. The extent and dynamics of this modification are highly reliant on lactate levels within the cellular microenvironment and can be modulated through the introduction of extracellular lactate in cultured cells or the stimulation of intracellular glycolysis. The introduction of lysine L-lactylation is mediated by the acetyltransferase p300, while the removal of lactylation marks from histones has been attributed to Class I histone deacetylases (HDAC 1-3). Histone lactylation has been implicated in various biological processes, including inflammation, fibrosis, differentiation, and cancer progression.

Cellular location

Nucleus

Images
Dot Blot

Peptide amount: 1 ng, 4 ng, 16 ng
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:10000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 30 seconds
The list of peptides used in the experiment is provided below.
Lane 1: H4K5 L-lactyl. Lane 2: H4K8 L-lactyl.
Lane 3: H4K12 L-lactyl. Lane 4: H4K5 crotonyl.
Lane 5: H4K8 crotonyl. Lane 6: H4K12 crotonyl.
Lane 7: H4K8 2-hydroxyisobutyryl. Lane 8: H4K12 2-hydroxyisobutyryl.
Lane 9: H4K5 unmodified. Lane 10: H4K5 2-hydroxyisobutyryl.
WB

Lysates: (-): HeLa cells; (+): HeLa cells + sodium lactate (100 mM, 24 hours)
Protein loading amount: 20 μg
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 10 seconds
Predicted band size: 11 kDa
Observed band size: 11 kDa

Lysates: HeLa, N2a, BRL
Protein loading amount: 20 μg
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 60 seconds
Predicted band size: 11 kDa
Observed band size: 11 kDa
ICC/IF

Samples: (A) HeLa cells; (B) HeLa cells + sodium lactate (100 mM, 24 hours)
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% Triton X-100
Primary Ab dilution: 1:100
Primary Ab incubation condition: 4°C overnight
Secondary Ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (blue)
Counter stain: Tubulin (red)
Description: The green color represents the positive signal observed with PTM-1407RM.
FC

Sample: HeLa cells
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% Triton X-100
Primary Ab dilution: 1:100
Secondary Ab: Goat Anti-Rabbit IgG
Unlabeled control: The cell without incubation with primary antibody and secondary antibody (black line).
Isotype control: Rabbit mAb IgG Isotype Control (blue line).
Description: The red line represents the positive signal observed with PTM-1407RM.
IP

IP of HeLa cells extracts
Lane 1: 5% Input
Lane 2: IP with Rabbit mAb IgG Isotype Control
Lane 3: IP with PTM-1407RM
IP Ab incubation condition: 4°C overnight, 1:50 dilution
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
WB primary Ab incubation condition: PTM-1407RM, 4°C overnight, 1:2000 dilution
Secondary Ab: Anti-Rabbit IgG for IP (HRP Conjugate)
Exposure time: 60 seconds
Predicted band size: 11 kDa
Observed band size: 11 kDa

Research Use

For research use only, not for use in diagnostic procedures.

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