中 / EN
Back
QUICK ORDER
Technical Support
| Contact Us
+1 312-802-6843
Technical Support
Contact Us
+1 312-802-6843
中 / EN
QUICK ORDER
Proteomics Bioinformation Research Antibody Products IVD Products Custom Antibody Development
Recover password
Professional Email
Verification Code
Send Code
Password
Recombinant Rabbit Monoclonal
Anti-L-Lactyl-Histone H4 (Lys5) Rabbit mAb
Catalog Number: PTM-1407RM
$ 420

Clone Number: 17H1L1

Host: Rabbit Clonality: Recombinant Monoclonal

Applications： WB ICC/IF FC IP

Reactivity: Human, Mouse, Rat

Synonyms: H4K5la

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms H4K5la
UniProt ID

P62805

Immunogen L-lactylated human histone H4 (Lys5) peptide
MW (kDa) 11
Specificity Anti-L-Lactyl-Histone H4 (Lys5) Rabbit mAb detects histone H4 only when it is L-lactylated at Lys5.
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:2000 Human, Mouse, Rat
ICC/IF 1:100 Human
FC 1:50 - 1:100 Human
IP 1:50 Human
Properties
Purity Protein A purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Histones, fundamental proteins involved in chromatin structure and gene regulation, are subject to a wide array of enzyme-catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitination, and numerous others. Histone L-lactylation, a recently discovered post-translational modification induced by lactate has emerged as a significant addition to this repertoire. The extent and dynamics of this modification are highly reliant on lactate levels within the cellular microenvironment and can be modulated through the introduction of extracellular lactate in cultured cells or the stimulation of intracellular glycolysis. The introduction of lysine L-lactylation is mediated by the acetyltransferase p300, while the removal of lactylation marks from histones has been attributed to Class I histone deacetylases (HDAC 1-3). Histone lactylation has been implicated in various biological processes, including inflammation, fibrosis, differentiation, and cancer progression.

Cellular location

Nucleus

Images
Dot Blot

Peptide amount: 1 ng, 4 ng, 16 ng
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:10000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 30 seconds
The list of peptides used in the experiment is provided below.
Lane 1: H4K5 lactyl. Lane 2: H4K8 lactyl.
Lane 3: H4K12 lactyl. Lane 4: H4K5 crotonyl.
Lane 5: H4K8 crotonyl. Lane 6: H4K12 crotonyl.
Lane 7: H4K8 2-hydroxyisobutyryl. Lane 8: H4K12 2-hydroxyisobutyryl.
Lane 9: H4K5 unmodified. Lane 10: H4K5 2-hydroxyisobutyryl.
WB

Lysates: (-): HeLa cells; (+): HeLa cells + sodium lactate (100 mM, 24 hours)
Protein loading amount: 20 μg
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 10 seconds
Predicted band size: 11 kDa
Observed band size: 11 kDa

Lysates: HeLa, N2a, BRL
Protein loading amount: 20 μg
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 60 seconds
Predicted band size: 11 kDa
Observed band size: 11 kDa
ICC/IF

Samples: (A) HeLa cells; (B) HeLa cells + sodium lactate (100 mM, 24 hours)
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% Triton X-100
Primary Ab dilution: 1:100
Primary Ab incubation condition: 4°C overnight
Secondary Ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (blue)
Counter stain: Tubulin (red)
Description: The green color represents the positive signal observed with PTM-1407RM.
FC

Sample: HeLa cells
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% Triton X-100
Primary Ab dilution: 1:100
Secondary Ab: Goat Anti-Rabbit IgG
Unlabeled control: The cell without incubation with primary antibody and secondary antibody (black line).
Isotype control: Rabbit mAb IgG Isotype Control (blue line).
Description: The red line represents the positive signal observed with PTM-1407RM.
IP

IP of HeLa cells extracts
Lane 1: 5% Input
Lane 2: IP with Rabbit mAb IgG Isotype Control
Lane 3: IP with PTM-1407RM
IP Ab incubation condition: 4°C overnight, 1:50 dilution
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
WB primary Ab incubation condition: PTM-1407RM, 4°C overnight, 1:2000 dilution
Secondary Ab: Anti-Rabbit IgG for IP (HRP Conjugate)
Exposure time: 60 seconds
Predicted band size: 11 kDa
Observed band size: 11 kDa

Research Use

For research use only, not for use in diagnostic procedures.

Recommended Products
Alternative Products Compatible Secondaries Isotype Control Writers/Readers/Erasers Related Products
  • View More Images（3）
    $ 435
    Anti-L-Lactyl-Histone H4 (Lys5) Rabbit pAb
    Catalog #
    PTM-1407
    Host
    Rabbit
    Clonality
    Polyclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Mouse, Rat
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
  • View More Images（4）
    $ 435
    Anti-L-Lactyl-Histone H4 (Lys5) Mouse mAb
    Catalog #
    PTM-1409
    Host
    Mouse
    Clonality
    Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Mouse, Rat
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
  • View More Images（4）
    $ 135
    Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
    Catalog #
    PTM-6261
    Host
    Goat
    Clonality
    Polyclonal
    Conjugate
    HRP
    Reactivity
    Rabbit
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
  • View More Images（5）
    $ 125
    Recombinant Rabbit Monoclonal
    Rabbit mAb IgG Isotype Control
    Catalog #
    PTM-5073
    Host
    Rabbit
    Clonality
    Recombinant Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Mouse, Rat
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
  • View More Images（5）
    $ 125
    Recombinant Rabbit Monoclonal
    Rabbit mAb IgG Isotype Control
    Catalog #
    PTM-6628
    Host
    Rabbit
    Clonality
    Recombinant Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Mouse
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
  • View More Images（6）
    $ 290
    Recombinant Rabbit Monoclonal
    Anti-HDAC1 Rabbit mAb
    Catalog #
    PTM-6409
    Host
    Rabbit
    Clonality
    Recombinant Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Mouse, Rat
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
  • View More Images（1）
    $ 280
    Recombinant
    Anti-HDAC2 Mouse mAb
    Catalog #
    PTM-7219
    Host
    Mouse
    Clonality
    Recombinant Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Mouse, Rat
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
  • View More Images（3）
    $ 280
    Recombinant
    Anti-HDAC3 Mouse mAb
    Catalog #
    PTM-6070
    Host
    Mouse
    Clonality
    Recombinant Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Mouse, Rat
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
  • View More Images（7）
    $ 420
    Anti-Butyryl-Histone H4 (Lys5) Mouse mAb
    Catalog #
    PTM-310
    Host
    Mouse
    Clonality
    Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Mouse, Rat
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
  • View More Images（2）
    $ 435
    Anti-Crotonyl-Histone H4 (Lys5) Rabbit pAb
    Catalog #
    PTM-521
    Host
    Rabbit
    Clonality
    Polyclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Mouse, Rat, Monkey
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
  • View More Images（8）
    $ 420
    Recombinant Rabbit Monoclonal
    Anti-Propionyl-Histone H4 (Lys5) Rabbit mAb
    Catalog #
    PTM-219RM
    Host
    Rabbit
    Clonality
    Recombinant Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Mouse, Rat
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
  • View More Images（3）
    $ 435
    Anti-2-Hydroxyisobutyryl-Histone H4 (Lys5) Mouse mAb
    Catalog #
    PTM-854
    Host
    Mouse
    Clonality
    Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Mouse, Rat
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
  • View More Images（2）
    $ 420
    Anti-β-Hydroxybutyryl-Histone H4 (Lys5) Rabbit pAb
    Catalog #
    PTM-1205
    Host
    Rabbit
    Clonality
    Polyclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Mouse, Rat
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
  • View More Images（12）
    $ 420
    Recombinant Rabbit Monoclonal
    Anti-L-Lactyllysine Rabbit mAb
    Catalog #
    PTM-1401RM
    Host
    Rabbit
    Clonality
    Recombinant Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    All
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
References (1)
  • Comprehensive Analysis of Lysine Lactylation in Rice (Oryza sativa) Grains
    Year 2021
    Journal JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
    Authors Xiaoxi Meng, et al.
    Applications Unspecified
    Reactivity
Service
Products
Support
About Us
© 2023 PTM Biolabs Inc.