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Recombinant Rabbit Monoclonal
Anti-L-Lactyl-Histone H4 (Lys5) Rabbit mAb
Catalog Number: PTM-1407RM
References (34)
$ 420

Clone Number: 17H1L1

Host: Rabbit Clonality: Recombinant Monoclonal

Applications： WB ICC/IF FC IP CUT&Tag

Reactivity: Human, Mouse, Rat

Synonyms: H4K5la

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms H4K5la
UniProt ID

P62805

Immunogen L-lactylated human histone H4 (Lys5) peptide
MW (kDa) 11
Specificity Anti-L-Lactyl-Histone H4 (Lys5) Rabbit mAb detects histone H4 only when it is L-lactylated at Lys5.
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:2000 Human, Mouse, Rat
ICC/IF 1:100 Human
FC 1:50 - 1:100 Human
IP 1:50 Human
CUT&Tag 1:100 Human
Properties
Purity Protein A purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Histones, fundamental proteins involved in chromatin structure and gene regulation, are subject to a wide array of enzyme-catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitination, and numerous others. Histone L-lactylation, a recently discovered post-translational modification induced by lactate has emerged as a significant addition to this repertoire. The extent and dynamics of this modification are highly reliant on lactate levels within the cellular microenvironment and can be modulated through the introduction of extracellular lactate in cultured cells or the stimulation of intracellular glycolysis. The introduction of lysine L-lactylation is mediated by the acetyltransferase p300, while the removal of lactylation marks from histones has been attributed to Class I histone deacetylases (HDAC 1-3). Histone lactylation has been implicated in various biological processes, including inflammation, fibrosis, differentiation, and cancer progression.

Cellular location

Nucleus

Images
Dot Blot

Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:10000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Rabbit IgG H&L (HRP)
Immunogen peptide quantity: 1 ng, 4 ng, 16 ng
Exposure time: 30 s
The list of peptides is included in the table below:
WB

Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:2000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: (-) HeLa, (+) HeLa+Sodium lactate (100mM, 24hr)
Protein loading quantity: 20 μg
Exposure time: 10 s
Predicted MW: 11 kDa
Observed MW: 11 kDa

Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:2000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: HeLa, N2a, BRL
Protein loading quantity: 20 μg
Exposure time: 10 s
Predicted MW: 11 kDa
Observed MW: 11 kDa
ICC/IF

Samples: (A) HeLa cells; (B) HeLa cells + sodium lactate (100 mM, 24 hours)
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% Triton X-100
Primary Ab dilution: 1:100
Primary Ab incubation: 4°C overnight
Secondary Ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (blue)
Counter stain: Tubulin (red)
Description: The green color represents the positive signal observed with PTM-1407RM.
FC

Cell line: HeLa
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% TritonX-100
Primary ab dilution: 1:100
Secondary ab: Goat anti Rabbit IgG
Unlabelled control: The cell without incubation
with primary antibody and secondary antibody (Black line).
Isotype control: Rabbit monoclonal IgG (Blue line).
Comment: Line red is the positive signal for PTM-1407RM
IP

IP of HeLa cells extracts
IP Ab incubation condition: PTM-1407RM, 4°C overnight, 1:50 dilution
WB primary Ab incubation condition: PTM1407RM, 4°C overnight, 1:2000 dilution
Secondary Ab: Anti-Rabbit IgG for IP (HRP)
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
1: 5% Input
2: IP with Rabbit monoclonal IgG Isotype
3: IP with PTM-1407RM
Observed MW: 11 kDa
Exposure time: 60 s
CUT&Tag

Cell type: HeLa + sodium lactate (100mM, 24h) (green), HeLa (blue)
Quantity of cell: 1×105 cells
Primary ab addition: 1 μg
Primary ab incubation condition: 4℃ Overnight
Secondary ab: Goat Anti-Rabbit IgG H&L pAb (PTM-6252)
Secondary ab addition: 1 μg
Description: Genomic visualization of CUT&Tag sequencing results from Normal Rabbit IgG negative control and Anti-LLactyl-Histone H4 (Lys5) Rabbit mAb (PTM1407RM). There are significant H4K5la signal enrichments at the regions of GAPDH, RAB20, LDHA. The DNA Libraries were sequenced by Illumina NovaSeq platform (PE 150) and the sequencing deep was 27 M reads/sample.

Research Use

For research use only, not for use in diagnostic procedures.

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References (34)
  • Lysine lactylation regulates ATF4-mediated stress responses under glucose starvation in canine hemangiosarcoma
    Year 2026
    Journal Frontiers in Veterinary Science
    Authors Tamami Suzuki, et al.
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  • Histone H4K8 lactylation promotes glioblastoma progression by inducing NUPR1-mediated autophagosome‒lysosome fusion
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    Journal Theranostics
    Authors Jiangli Zhao, et al.
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  • Histone H4 lysine 5 lactylation: A key regulator of immune metabolism in microglia during ischemic stroke
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    Journal PHARMACOLOGICAL RESEARCH
    Authors Yishan Li, et al.
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  • Histone Lactylation Couples FSH-Driven Lactate Metabolism to Mitochondrial Biogenesis by Enhancing HDAC4-Mediated Deacetylation of PGC-1α in Granulosa Cells
    Year 2026
    Journal Research
    Authors Gang Wu, et al.
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  • Lactate represses MHC-I antigen presentation via H4K5 lactylation-PRC2 in small cell lung cancer subtype A
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    Journal INTERNATIONAL IMMUNOPHARMACOLOGY
    Authors Yifan Cai, et al.
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  • Butyrate ameliorates lung inflammation in chronic obstructive pulmonary disease in association with the regulation of histone lactylation: A mechanistic study
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    Authors Min Jiang, et al.
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  • Histone Lactylation-Mediated PAD4 Up-Regulation Promotes Septic Acute Kidney Injury via Activating NETosis
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    Journal NEPHROLOGY
    Authors Wen-lin Huang, et al.
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    Journal AGING CELL
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  • H3K18 lactylation-hexokinase 2 positive feedback loop promotes osteogenesis of ASPCs in facial infiltrating lipomatosis
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  • Royal jelly acid inhibits NF-κB signaling by regulating H3 histone lactylation to alleviate IgE-mediated mast cell activation and allergic inflammation
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    Authors Xuhui Ge, et al.
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    Authors Yufang Dai, et al.
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    Authors Deng Jiao, et al.
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