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Recombinant Rabbit Monoclonal
Anti-L-Lactyl-Histone H4 (Lys8) Rabbit mAb
Catalog Number: PTM-1415RM
References (43)
$ 435

Clone Number: 24H4L5

Host: Rabbit Clonality: Recombinant Monoclonal

Applications： WB ChIP CUT&Tag

Reactivity: Human, Mouse, Rat

Synonyms: H4K8la

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms H4K8la
UniProt ID

P62805

Immunogen L-lactylated human histone H4 (Lys8) peptide
MW (kDa) 11
Specificity Anti-L-Lactyl-Histone H4 (Lys8) Rabbit mAb detects endogenous levels of histone H3 only when it is lactylated at Lys8.
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:2000 Human, Mouse, Rat
ChIP 6 μg per 5x106 cells Human
CUT&Tag 1:100 Human
Properties
Purity Protein A purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Histones, fundamental proteins involved in chromatin structure and gene regulation, are subject to a wide array of enzyme-catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitination, and numerous others. Histone L-lactylation, a recently discovered post-translational modification induced by lactate has emerged as a significant addition to this repertoire. The extent and dynamics of this modification are highly reliant on lactate levels within the cellular microenvironment and can be modulated through the introduction of extracellular lactate in cultured cells or the stimulation of intracellular glycolysis. The introduction of lysine L-lactylation is mediated by the acetyltransferase p300, while the removal of lactylation marks from histones has been attributed to Class I histone deacetylases (HDAC 1-3). Histone lactylation has been implicated in various biological processes, including inflammation, fibrosis, differentiation, and cancer progression.

Cellular location

Nucleus

Images
Dot Blot

Peptide amount: 4 ng, 16 ng, 64 ng
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 30 seconds
The list of peptides used in the experiment is provided below.
Lane 1: H4K8 L-lactyl. Lane 2: H4K8 2-hydroxyisobutyryl.
Lane 3: H4K12 crotonyl. Dot 4: H4K8 crotonyl.
Dot 5: H4K5 crotonyl. Dot 6: H4K12 2-hydroxyisobutyryl.
Dot 7: H4K16 L-lactyl. Dot 8: H4K5 L-lactyl.
Dot 9: H4K8 unmodified.
WB

Lysates: (-) HeLa cells; (+) HeLa cells treated with 100 mM sodium lactate for 24 hours
Protein loading amount: 20 μg
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 60 seconds
Predicted band size: 11 kDa
Observed band size: 11 kDa

Lysates: HeLa, NIH/3T3, BRL cells
Protein loading amount: 20 μg
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 60 seconds
Predicted band size: 11 kDa
Observed band size: 11 kDa
ChIP

Sample: HeLa cells treated with 100 mM sodium lactate for 24 hours
Cross-linking conditions: no cross-linking
Amount of chromatin per IP: 5×106 cells
Amount of Ab per IP: 6 μg
Beads type and amount per IP: 50 μL of Protein A MagBeads
Description: Chromatin immunoprecipitations were performed with 6 μg of normal rabbit IgG as a negative control. The immunoprecipitated DNA was quantified by real-time PCR using primers specific for the human GAPDH CDDS, RPL30, LDHA promoter, LDHA CDS, FOXO3a promoter, FOXO3a downstream, RAB20, and TUBBP10 regions. The data are presented as enrichment of each sample relative to the total amount of input at each amplicon.
CUT&Tag

Sample: HeLa cells treated with 25 mM sodium lactate for 24 hours
Cell quantity: 1×105 cells
Primary Ab dilution: 1:100
Primary Ab incubation: 4⁰C overnight
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (PTM-6252)
Secondary Ab dilution: 1:1400
Description: CUT&Tag was performed with Anti-L-Lactyl-Histone H4 (Lys8) Rabbit mAb (PTM-1415RM) alongside normal rabbit IgG as a negative control. The resulting DNA was sequenced on the Illumina NovaSeq platform with paired-end reads of 150 bp and a sequencing depth of 3 million reads. The figure shows significant H4K8la signal enrichments at the promoter regions of CD9, PLEKHG6, LTBR, and GAPDH.

Research Use

For research use only, not for use in diagnostic procedures.

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    $ 435
    Recombinant Rabbit Monoclonal
    Anti-L-Lactyl-Histone H4 (Lys8) Rabbit pAb
    Catalog #
    PTM-1415
    Host
    Rabbit
    Clonality
    Polyclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Mouse, Rat
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P CUT&Tag
  • View More Images（4）
    $ 135
    Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
    Catalog #
    PTM-6261
    Host
    Goat
    Clonality
    Polyclonal
    Conjugate
    HRP
    Reactivity
    Rabbit
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P CUT&Tag
References (43)
  • m5C-Modified tRF3b-CysGCA-23 Suppresses Bladder Cancer Malignancy by Repressing H3K18 Lactylation via Stabilizing RBM4
    Year 2026
    Journal Advanced Science
    Authors Xiaoling Ying, et al.
    Applications Unspecified
    Reactivity
  • BRD4 orchestrates the metabolic-epigenetic regulation of GM-CSF expression and secretion to drive PD-L1⁺ macrophage-mediated immune evasion in triple-negative breast cancer
    Year 2026
    Journal ONCOGENE
    Authors Diao Haizhou, et al.
    Applications WB, IHC-P, IF, IP, ChIP
    Reactivity
  • STING controls glycolysis and histone lactylation to drive macrophage metabolic reprogramming in postoperative ileus
    Year 2026
    Journal Communications Biology
    Authors Kai Chen, et al.
    Applications Unspecified
    Reactivity
  • Histone H4K8 lactylation promotes glioblastoma progression by inducing NUPR1-mediated autophagosome‒lysosome fusion
    Year 2026
    Journal Theranostics
    Authors Jiangli Zhao, et al.
    Applications IHC-P, IF, Co-IP
    Reactivity
  • Histone H4 lysine 5 lactylation: A key regulator of immune metabolism in microglia during ischemic stroke
    Year 2026
    Journal PHARMACOLOGICAL RESEARCH
    Authors Yishan Li, et al.
    Applications Unspecified
    Reactivity
  • Microvascular endothelial metabolic dysfunction drives cerebral edema through bioenergetic failure after ischemia-reperfusion
    Year 2026
    Journal Theranostics
    Authors Yi-Fan Zhou, et al.
    Applications WB
    Reactivity
  • ANGPT2/Tie2 Enhances H3K18la-Mediated Macrophage M2 Polarization to Promote Endothelial Cell Proliferation in the Chronically Ischaemic Brain
    Year 2026
    Journal CNS Neurosci Ther
    Authors Chuyang Tai
    Applications WB
    Reactivity
  • IGF2BP3 Promotes Head and Neck Squamous Cell Carcinoma Progression via the M6A-LDHA-Lactate-H4K8la Axis and E2F2 Activation
    Year 2026
    Journal Technol Cancer Res Treat
    Authors Fan Wu, et al.
    Applications WB, IF, CUT&Tag
    Reactivity
  • Histone lactylation in fibroblast-like synoviocytes drives CXCL1-mediated 2 immune cells recruitment in autoimmune arthritis
    Year 2026
    Journal Available at SSRN
    Authors Zhidong Liu, et al.
    Applications CUT&Tag
    Reactivity
  • SIRT6-mediated immunometabolic reprogramming of macrophages drives neutrophilic asthma via LDHA-dependent glycolysis
    Year 2026
    Journal Cell Rep
    Authors Guomei Su, et al.
    Applications Unspecified
    Reactivity
  • Carnosol targets KAT2B/H3K18la axis to regulate histone 2 lactylation and inhibit proliferation of lung cancer cells
    Year 2026
    Journal SSRN
    Authors Minfu Liu, et al.
    Applications WB
    Reactivity
  • Activating the Osteoblastic USP26 Pathway Alleviates Multi-Organ Fibrosis by Decreasing Insulin Resistance
    Year 2025
    Journal Advanced Science
    Authors Jiyuan Tang, et al.
    Applications Unspecified
    Reactivity
  • H3K18 lactylation in cancer-associated fibroblasts drives malignant pleural effusion progression via TNFR2+ Treg recruitment
    Year 2025
    Journal EXPERIMENTAL AND MOLECULAR MEDICINE
    Authors Ye Linlin, et al.
    Applications WB
    Reactivity
  • Hexokinase 2-mediated histone H3K18la promotes PAI-1-dependent thrombosis in acute myeloid leukemia via tumor-endothelial crosstalk
    Year 2025
    Journal Nature Communications
    Authors Bai Qin, et al.
    Applications WB
    Reactivity
  • Histone H4K8 lactylation modulated immunosuppressive properties by promoting FAP transcription and ECM remodeling
    Year 2025
    Journal Gastric Cancer
    Authors Huang Chao, et al.
    Applications Unspecified
    Reactivity
  • Histone Lactylation-Mediated PAD4 Up-Regulation Promotes Septic Acute Kidney Injury via Activating NETosis
    Year 2025
    Journal NEPHROLOGY
    Authors Wen-lin Huang, et al.
    Applications Unspecified
    Reactivity
  • TRPM7 Deficiency Accelerates Vascular Senescence by Inhibiting H3K18 Lactylation
    Year 2025
    Journal AGING CELL
    Authors Yue Wang, et al.
    Applications WB
    Reactivity
  • Elevated lactate production exacerbates PM2.5-induced pulmonary fibrosis by stabilizing TGF-β1
    Year 2025
    Journal Journal of Advanced Research
    Authors Zhihao Liu, et al.
    Applications Unspecified
    Reactivity
  • H3K14 lactylation exacerbates neuronal ferroptosis by inhibiting calcium efflux following intracerebral hemorrhagic stroke
    Year 2025
    Journal Cell Death & Disease
    Authors Sun Tingting, et al.
    Applications WB, IF
    Reactivity
  • H3K18 lactylation drives the progression of silica nanoparticles-induced pulmonary fibrosis via promoting macrophage M1 polarization
    Year 2025
    Journal JOURNAL OF HAZARDOUS MATERIALS
    Authors Lili Shi, et al.
    Applications Unspecified
    Reactivity
  • Lactate promotes collagen expression, proliferation and migration through H3K18 lactylation-dependent stimulation of LTBP3/TGF-β1 axis in keloid fibroblasts
    Year 2025
    Journal JOURNAL OF INVESTIGATIVE DERMATOLOGY
    Authors Jing-Jing Gu, et al.
    Applications Unspecified
    Reactivity
  • Quantitative Proteomics Reveals the Role of Lysine Lactylation in Lenalidomide-Resistance in Multiple Myeloma Cells
    Year 2025
    Journal ACS Chemical Biology
    Authors Xinlong Guo, et al.
    Applications Unspecified
    Reactivity
  • WTAP Mediated m6A Modification Stabilizes PDIA3P1 and Promotes Tumor Progression Driven by Histone Lactylation in Esophageal Squamous Cell Carcinoma
    Year 2025
    Journal Advanced Science
    Authors Tao Huang, et al.
    Applications Unspecified
    Reactivity
  • Histone lactylation-induced premature senescence contributes to 1-nitropyrene-Induced chronic obstructive pulmonary disease
    Year 2025
    Journal Redox Biology
    Authors Rong-Rong Wang, et al.
    Applications Unspecified
    Reactivity
  • Gastrodin attenuates rheumatoid arthritis by targeting KAT8 to inhibit the lactylation of H3K9
    Year 2025
    Journal Frontiers in Pharmacology
    Authors Yufang Dai, et al.
    Applications Unspecified
    Reactivity
  • Histone lactylation enhances GCLC expression and thus promotes chemoresistance of colorectal cancer stem cells through inhibiting ferroptosis
    Year 2025
    Journal Cell Death & Disease
    Authors Deng Jiao, et al.
    Applications Unspecified
    Reactivity
  • Histone lactylation stimulated upregulation of PSMD14 alleviates neuron PANoptosis through deubiquitinating PKM2 to activate PINK1-mediated mitophagy after traumatic brain injury
    Year 2025
    Journal Autophagy
    Authors Lei Xu, et al.
    Applications Unspecified
    Reactivity
  • Metabolic reprogramming in astrocytes prevents neuronal death through a UCHL1/PFKFB3/H4K8la positive feedback loop
    Year 2025
    Journal CELL DEATH AND DIFFERENTIATION
    Authors Xiong Junjun, et al.
    Applications Unspecified
    Reactivity
  • H4K12 lactylation potentiates mitochondrial oxidative stress via the Foxo1 pathway in diabetes-induced cognitive impairment
    Year 2025
    Journal Journal of Advanced Research
    Authors Ying Yang, et al.
    Applications WB, IF
    Reactivity
  • Lgals3 Promotes Calcium Oxalate Crystal Formation and Kidney Injury Through Histone Lactylation-Mediated FGFR4 Activation
    Year 2025
    Journal Advanced Science
    Authors Zehua Ye, et al.
    Applications Unspecified
    Reactivity
  • Enhanced glycolysis-derived lactate promotes microglial activation in Parkinson’s disease via histone lactylation
    Year 2025
    Journal npj Parkinsons Disease
    Authors Qin Qixiong, et al.
    Applications WB
    Reactivity
  • Epigenetic regulation of cardiac tissue development by lysine lactylation
    Year 2025
    Journal hLife
    Authors Xiaodong Luan, et al.
    Applications Unspecified
    Reactivity
  • H4K12 Lactylation Activated-Spp1 in Reprogrammed Microglia Improves Functional Recovery After Spinal Cord Injury
    Year 2025
    Journal CNS Neurosci Ther
    Authors Xiaokun Wang, et al.
    Applications WB
    Reactivity
  • Hypoxia-induced PYCR1 regulates glycolysis and histone lactylation to promote bladder cancer progression and metastasis via SLC6A14/Glutamine metabolism
    Year 2025
    Journal Cancer Biol Ther
    Authors Zhuo Li, et al.
    Applications WB
    Reactivity
  • DNAJC12 downregulation induces neuroblastoma progression via increased histone H4K5 lactylation
    Year 2024
    Journal Journal of Molecular Cell Biology
    Authors Yang Yaqi, et al.
    Applications Unspecified
    Reactivity
  • Dual activation of GCGR/GLP1R signaling ameliorates intestinal fibrosis via metabolic regulation of histone H3K9 lactylation in epithelial cells
    Year 2024
    Journal Acta Pharmaceutica Sinica B
    Authors Han Liu, et al.
    Applications Unspecified
    Reactivity
  • H3K9 lactylation in malignant cells facilitates CD8+ T cell dysfunction and poor immunotherapy response
    Year 2024
    Journal Cell Reports
    Authors Ruijie Wang, et al.
    Applications WB
    Reactivity
  • Lactylation of histone by BRD4 regulates astrocyte polarization after experimental subarachnoid hemorrhage
    Year 2024
    Journal Journal of Neuroinflammation
    Authors Zhang Fan, et al.
    Applications WB, IF, IP
    Reactivity
  • Histone H3K18 and Ezrin Lactylation Promote Renal Dysfunction in Sepsis-Associated Acute Kidney Injury
    Year 2024
    Journal Advanced Science
    Authors Jiao Qiao, et al.
    Applications Unspecified
    Reactivity
  • Single-embryo transcriptomic atlas of oxygen response reveals the critical role of HIF-1α in prompting embryonic zygotic genome activation
    Year 2024
    Journal Redox Biology
    Authors Fusheng Yao, et al.
    Applications Unspecified
    Reactivity
  • Proteomic analysis identifies PFKP lactylation in SW480 colon cancer cells
    Year 2024
    Journal iScience
    Authors Zhe Cheng
    Applications WB
    Reactivity
  • The diapause-like colorectal cancer cells induced by SMC4 attenuation are characterized by low proliferation and chemotherapy insensitivity
    Year 2023
    Journal Cell Metabolism
    Authors Xuedan Sun, et al.
    Applications Unspecified
    Reactivity
  • STAT5 promotes PD-L1 expression by facilitating histone lactylation to drive immunosuppression in acute myeloid leukemia
    Year 2023
    Journal Signal Transduction and Targeted Therapy
    Authors Huang Ze-Wei, et al.
    Applications WB
    Reactivity
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