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Recombinant Rabbit Monoclonal
Anti-L-Lactyl-Histone H4 (Lys8) Rabbit mAb
Catalog Number: PTM-1415RM
$ 435

Clone Number: 24H4L5

Host: Rabbit Clonality: Recombinant Monoclonal

Applications： WB ChIP CUT&Tag

Reactivity: Human, Mouse, Rat

Synonyms: H4K8la

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms H4K8la
UniProt ID

P62805

Immunogen L-lactylated human histone H4 (Lys8) peptide
MW (kDa) 11
Specificity Anti-L-Lactyl-Histone H4 (Lys8) Rabbit mAb detects endogenous levels of histone H3 only when it is lactylated at Lys8.
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:2000 Human, Mouse, Rat
ChIP 4 μg/5x106 cells Human
CUT&Tag 1:100 Human
Properties
Purity Protein A purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Histones are subjected to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation,ubiquitylation, etc. The Warburg effect, which originally described increased production of lactate in cancer, is associated with diverse cellular processes such as angiogenesis, hypoxia, polarization of macrophages and activation of T cells. This phenomenon is intimately linked to several diseases including neoplasia, sepsis and autoimmune diseases.Lactate, which is converted from pyruvate in tumour cells, is widely known as an energy source and metabolic by-product. Zhang et al. (Nature, 2019) reveal that histone lactylation is a previously unknown histone modification,derived from the cellular metabolite lactate.

Cellular location

Nucleus

Images
Dot Blot

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Peptide amount: 4 ng, 16 ng, 64 ng
Exposure time: 30 s
The list of peptides is included in the table below.
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Lysate: (-) HeLa; (+) HeLa+ sodium lactate (100 mM, 24 hours)
Protein loading amount: 20 μg
Exposure time: 60 s
Predicted band size: 11 kDa
Observed band size: 11 kDa

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Lysate: HeLa, NIH/3T3, BRL
Protein loading amount: 20 μg
Exposure time: 60 s
Predicted band size: 11 kDa
Observed band size: 11 kDa
ChIP

Cell type: HeLa+ sodium lactate (100 mM, 24 hours)
Cross-linking conditions: No cross-linking
Amount of chromatin per IP: 5x106 cells
Amount of Ab per IP: 4ug, 12ug
Beads type and amount per IP: 50 μl of Protein A/G MagBeads
Description: Chromatin immunoprecipitations were performed with 1 μg of normal rabbit IgG as a negative control. The immunoprecipitated DNA was quantified by real-time PCR using primers specific for the human GAPDH CDS region, LDHA, RPL30 Exon 3, FOXO3a-promoter and FOXO3adownstream. The data are presented as enrichment of each sample relative to total amount of input chromatin at each amplicon.
CUT&Tag

Cell type: HeLa + sodium lactate (100 mM, 24hr)
Quantity of cell: 1×105 cells
Primary ab dilution: 1: 100
Primary ab incubation condition: 4℃ overnight
Secondary ab: Goat Anti-Rabbit IgG H&L pAb (PTM-6252)
Secondary ab dilution: 1: 1400
Description: Genomic visualization of CUT&Tag sequencing results from Normal Rabbit IgG negative control and Anti-L-Lactyl-Histone H4 (Lys8) Rabbit mAb (PTM-1415RM) . There are significant H4K8la signal enrichments at the promoter regions of CD9、PLEKHG6、LTBR and GAPDH. DNA Libraries were sequenced with Illumina NovaSeq platform (PE 150) and sequencing depth of 3 M reads.

Research Use

For research use only, not for use in diagnostic procedures.

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  • View More Images（4）
    $ 435
    Anti-L-Lactyl-Histone H4 (Lys8) Rabbit pAb
    Catalog #
    PTM-1415
    Host
    Rabbit
    Clonality
    Polyclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Mouse, Rat
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
  • View More Images（4）
    $ 135
    Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
    Catalog #
    PTM-6261
    Host
    Goat
    Clonality
    Polyclonal
    Conjugate
    HRP
    Reactivity
    Rabbit
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
References (1)
  • The diapause-like colorectal cancer cells induced by SMC4 attenuation are characterized by low proliferation and chemotherapy insensitivity
    Year 2023
    Journal Cell Metabolism
    Authors Xuedan Sun, et al.
    Applications Unspecified
    Reactivity
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