Applications：
WB
IHC-P
ICC/IF
FC
IAP
IP
ChIP
mIHC-P
Clone Number: /
Host: Rabbit Clonality: Polyclonal
Applications： WB ChIP
Reactivity: Human, Mouse, Rat
Synonyms: H4K8la
Shipping: Ambient temperature
Order online or send purchase order to info@ptmbio.com
| Isotype | IgG |
| Conjugate | Unconjugated |
| Synonyms | H4K8la |
| UniProt ID | |
| Immunogen | L-lactylated human histone H4 (Lys8) peptide |
| MW (kDa) | 11 |
| Specificity | Anti-L-Lactyl-Histone H4 (Lys8) Rabbit mAb detects endogenous levels of histone H3 only when it is lactylated at Lys8. |
| Applications | Dilution | Recommended Species |
|---|---|---|
| WB | 1:500 - 1:2000 | Human, Mouse, Rat |
| ChIP | 1:50 - 1:200 | Human |
| Purity | Protein A and immunogen affinity purified |
| Constituents | PBS, Glycerol, BSA |
| Storage | Store at -20°C. Avoid freeze/thaw cycles. |
| Stability | Stable for 12 months from date of receipt/reconstitution. |
Background
Histones are subjected to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation,ubiquitylation, etc. The Warburg effect, which originally described increased production of lactate in cancer, is associated with diverse cellular processes such as angiogenesis, hypoxia, polarization of macrophages and activation of T cells. This phenomenon is intimately linked to several diseases including neoplasia, sepsis and autoimmune diseases.Lactate, which is converted from pyruvate in tumour cells, is widely known as an energy source and metabolic by-product. Zhang et al. (Nature, 2019) reveal that histone lactylation is a previously unknown histone modification,derived from the cellular metabolite lactate.
Cellular location
Nucleus
| Dot Blot | |
|---|---|
|
|
Blocking buffer: 5% NFDM/TBST |
| WB | |
|---|---|
|
|
Blocking buffer: 5% NFDM/TBST |
|
|
Blocking buffer: 5% NFDM/TBST |
| ChIP | |
|---|---|
|
|
Cell type: HeLa |
Research Use
For research use only, not for use in diagnostic procedures.
