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Anti-L-Lactyllysine Antibody Conjugated Agarose Beads
Catalog Number: PTM-1404
$ 3,940

Clone Number: /

Host: Rabbit Clonality: /

Applications： IAP

Reactivity: All

Synonyms: Kla, L-la

Product Size
500 μl
Quantity

Shipping: Cold packs / dry ice

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Agarose
Synonyms Kla, L-la
UniProt ID

/

Immunogen L-lactylated lysine peptides
MW (kDa) Multiple
Specificity With the immobilization of highly specific Anti-L-Lactyllysine Antibodies, the Anti-L-Lactyllysine Antibody Conjugated Agarose Beads selectively capture peptides/proteins bearing L-lactyllysine residues, but do not cross-react with the peptides/proteins bearing other structurally similar modified residues. This product has been well utilized to affinity purify peptides for global proteomic screening of lysine lactylation.
Product Usage Information
Applications Dilution Recommended Species
IAP 40 μl beads per 2 mg peptides All
Properties
Purity /
Constituents Antibody conjugated agarose beads are supplied as a 50% slurry containing 66% glycerol and 4 mg/ml immobilized antibody.
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt.
Target Information

Background

Lactate, previously recognized solely as an energy source and metabolic byproduct, has now emerged as a crucial player in cancer biology, especially within the scope of the Warburg effect and its intricate interplay with various cellular processes, including angiogenesis, hypoxia, polarization of macrophages, and T cell activation. Recent investigations have unveiled an unprecedented function of lactate in the realm of histone modification, specifically through lysine L-lactylation, which exerts regulatory control over gene expression. The extent and dynamics of this modification are highly reliant on lactate levels within the cellular microenvironment and can be modulated through the introduction of extracellular lactate in cultured cells or the stimulation of intracellular glycolysis. The acetyltransferase p300 is responsible for introducing lysine L-lactylation, while Class I histone deacetylases (HDAC 1-3) have been identified as an eraser of the lactylation marks on histones.

Cellular location

/

Images
Dot Blot

Dot blot analysis of Anti-L-Lactyllysine Antibody on modified and unmodiifed peptides.
Peptide amount: 4 ng, 20 ng, 50 ng, 200 ng
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 60 seconds
The list of peptides used in the experiment is provided below.
Lane 1: L-lactylated peptide library. Lane 2: D-lactylated peptide library.
Lane 3: Carboxyethylated peptide library. Lane 4: Acetylated peptide library.
Lane 5: Butyrylated peptide library. Lane 6: Crotonylated peptide library.
Lane 7: 2-Hydroxyisobutyrylated peptide library. Lane 8: Succinylated peptide library.
Lane 9: Crotonylated peptide library.

Research Use

For research use only, not for use in diagnostic procedures.

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References (41)
  • Comprehensive analysis of lysine lactylation and its potential biological significance in clear cell renal cell carcinoma
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    Journal EUROPEAN JOURNAL OF MEDICAL RESEARCH
    Authors Bangbei Wan, et al.
    Applications Unspecified
    Reactivity
  • Nuclear GTPSCS functions as a lactyl-CoA synthetase to promote histone lactylation and gliomagenesis
    Year 2024
    Journal Cell Metabolism
    Authors Ruilong Liu, et al.
    Applications Unspecified
    Reactivity
  • PTBP1 Lactylation Promotes Glioma Stem Cell Maintenance through PFKFB4-Driven Glycolysis
    Year 2024
    Journal CANCER RESEARCH
    Authors Zhou Zijian, et al.
    Applications Unspecified
    Reactivity
  • Post-translational toxin modification by lactate controls Staphylococcus aureus virulence
    Year 2024
    Journal Nature Communications
    Authors Wang Yanan, et al.
    Applications Unspecified
    Reactivity
  • Tufm lactylation regulates neuronal apoptosis by modulating mitophagy in traumatic brain injury
    Year 2024
    Journal CELL DEATH AND DIFFERENTIATION
    Authors Weng Weiji, et al.
    Applications Unspecified
    Reactivity
  • Aloe Emodin Alleviates Radiation-Induced Heart Disease via Blocking P4HB Lactylation and Mitigating Kynurenine Metabolic Disruption
    Year 2024
    Journal Advanced Science
    Authors Fan Ouyang, et al.
    Applications Unspecified
    Reactivity
  • Lactylation stabilizes TFEB to elevate autophagy and lysosomal activity
    Year 2024
    Journal JOURNAL OF CELL BIOLOGY
    Authors Yewei Huang, et al.
    Applications Unspecified
    Reactivity
  • Lactylation-Driven IGF2BP3-Mediated Serine Metabolism Reprogramming and RNA m6A—Modification Promotes Lenvatinib Resistance in HCC
    Year 2024
    Journal Advanced Science
    Authors Yuanxiang Lu, et al.
    Applications Unspecified
    Reactivity
  • Multi-omics reveals lactylation-driven regulatory mechanisms promoting tumor progression in oral squamous cell carcinoma
    Year 2024
    Journal GENOME BIOLOGY
    Authors Jing Fengyang, et al.
    Applications Unspecified
    Reactivity
  • Systematic Analysis of Lysine Lactylation in Nucleus Pulposus Cells
    Year 2024
    Journal iScience
    Authors Lei Sheng, et al.
    Applications Unspecified
    Reactivity
  • AARS1 and AARS2 sense l-lactate to regulate cGAS as global lysine lactyltransferases
    Year 2024
    Journal NATURE
    Authors Li Heyu, et al.
    Applications Unspecified
    Reactivity
  • Global profiling of protein lactylation in microglia in experimental high-altitude cerebral edema
    Year 2024
    Journal Cell Communication and Signaling
    Authors Jiang Xiufang, et al.
    Applications Unspecified
    Reactivity
  • Lysine lactylation-based insight to understanding the characterization of cervical cancer
    Year 2024
    Journal BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
    Authors Chaoran He, et al.
    Applications Unspecified
    Reactivity
  • Integrative Analysis of Lactylome and Proteome of Hypertrophic Scar To Identify Pathways or Proteins Associated with Disease Development
    Year 2024
    Journal JOURNAL OF PROTEOME RESEARCH
    Authors Enyuan Zhang, et al.
    Applications Unspecified
    Reactivity
  • Lactylated Apolipoprotein C-II Induces Immunotherapy Resistance by Promoting Extracellular Lipolysis
    Year 2024
    Journal Advanced Science
    Authors Jian Chen, et al.
    Applications Unspecified
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  • Methyl-CpG-binding 2 K271 lactylation-mediated M2 macrophage polarization inhibits atherosclerosis
    Year 2024
    Journal Theranostics
    Authors Liangqi Chen, et al.
    Applications Unspecified
    Reactivity
  • p53 lysine-lactylated modification contributes to lipopolysaccharide-induced proinflammatory activation in BV2 cell under hypoxic conditions
    Year 2024
    Journal NEUROCHEMISTRY INTERNATIONAL
    Authors Xuechao Fei, et al.
    Applications Unspecified
    Reactivity
  • Astrocytic LRP1 enables mitochondria transfer to neurons and mitigates brain ischemic stroke by suppressing ARF1 lactylation
    Year 2024
    Journal Cell Metabolism
    Authors Jian Zhou, et al.
    Applications Unspecified
    Reactivity
  • Protein Lactylation Profiles Provide Insights into Molecular Mechanisms Underlying Metabolism in Yak
    Year 2024
    Journal JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
    Authors Zhijuan Wu, et al.
    Applications Unspecified
    Reactivity
  • Lactylome analyses suggest systematic lysine-lactylated substrates in oral squamous cell carcinoma under normoxia and hypoxia
    Year 2024
    Journal CELLULAR SIGNALLING
    Authors Fan Song, et al.
    Applications Unspecified
    Reactivity
  • Multi-omics explores the potential regulatory role of acetylation modification in flavonoid biosynthesis of Ginkgo biloba
    Year 2024
    Journal TREE PHYSIOLOGY
    Authors Liu Xiaomeng, et al.
    Applications Unspecified
    Reactivity
  • Lactylproteome analysis indicates histone H4K12 lactylation as a novel biomarker in triple-negative breast cancer
    Year 2024
    Journal Frontiers in Endocrinology
    Authors Cui Zhaolei, et al.
    Applications Unspecified
    Reactivity
  • Glucose-driven histone lactylation promotes the immunosuppressive activity of monocyte-derived macrophages in glioblastoma
    Year 2024
    Journal IMMUNITY
    Authors Alessandra De Leo, et al.
    Applications Unspecified
    Reactivity
  • ACSF2 and lysine lactylation contribute to renal tubule injury in diabetes
    Year 2024
    Journal DIABETOLOGIA
    Authors Chen Jingfang, et al.
    Applications Unspecified
    Reactivity
  • Nucleolin lactylation contributes to intrahepatic cholangiocarcinoma pathogenesis via RNA splicing regulation of MADD
    Year 2024
    Journal JOURNAL OF HEPATOLOGY
    Authors Long Yang, et al.
    Applications Unspecified
    Reactivity
  • HBO1 catalyzes lysine lactylation and mediates histone H3K9la to regulate gene transcription
    Year 2024
    Journal Nature Communications
    Authors Niu Ziping, et al.
    Applications Unspecified
    Reactivity
  • Alanyl-tRNA synthetase, AARS1, is a lactate sensor and lactyltransferase that lactylates p53 and contributes to tumorigenesis
    Year 2024
    Journal CELL
    Authors Zhi Zong, et al.
    Applications Unspecified
    Reactivity
  • α-myosin heavy chain lactylation maintains sarcomeric structure and function and alleviates the development of heart failure
    Year 2023
    Journal CELL RESEARCH
    Authors Zhang Naijin, et al.
    Applications Unspecified
    Reactivity
  • Mitochondrial pyruvate carrier 1 regulates fatty acid synthase lactylation and mediates treatment of nonalcoholic fatty liver disease
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    Journal HEPATOLOGY
    Authors Gao Ruxin, et al.
    Applications Unspecified
    Reactivity
  • cKMT1 is a new lysine methyltransferase that methylates the ferredoxin-NADP(+) oxidoreductase (FNR) and regulates energy transfer in cyanobacteria
    Year 2023
    Journal MOLECULAR & CELLULAR PROTEOMICS
    Authors Gaoxiang Cao, et al.
    Applications Unspecified
    Reactivity
  • Novel Insight of Nitrogen Deprivation Affected Lipid Accumulation by Genome-Wide Lactylation in Nannochloropsis oceanica
    Year 2023
    Journal JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
    Authors Jiao Wang, et al.
    Applications Unspecified
    Reactivity
  • Deciphering the Atlas of Post-Translational Modification in Sugarcane
    Year 2023
    Journal JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
    Authors Qibin Wu, et al.
    Applications Unspecified
    Reactivity
  • Lactylome Analyses Suggest Systematic Lysine-Lactylated Substrates in Oral Squamous Cell Carcinoma Under Normoxia and Hypoxia
    Year 2023
    Journal iScience
    Authors Song Fan, et al.
    Applications Unspecified
    Reactivity
  • Multi-Proteomic Analysis Reveals the Effect of Protein Lactylation on Matrix and Cholesterol Metabolism in Tendinopathy
    Year 2023
    Journal JOURNAL OF PROTEOME RESEARCH
    Authors Yuan Lin, et al.
    Applications Unspecified
    Reactivity
  • Global Profiling of Lysine Acetylation and Lactylation in Kupffer Cells
    Year 2023
    Journal JOURNAL OF PROTEOME RESEARCH
    Authors Eunji Sung, et al.
    Applications Unspecified
    Reactivity
  • Global profiling of protein lactylation in Caenorhabditis elegans
    Year 2023
    Journal PROTEOMICS
    Authors Tao Ding, et al.
    Applications Unspecified
    Reactivity
  • cKMT1 is a New Lysine Methyltransferase That Methylates the Ferredoxin-NADP(+) Oxidoreductase and Regulates Energy Transfer in Cyanobacteria
    Year 2023
    Journal MOLECULAR & CELLULAR PROTEOMICS
    Authors Gaoxiang Cao, et al.
    Applications Unspecified
    Reactivity
  • YiaC and CobB regulate lysine lactylation in Escherichia coli
    Year 2022
    Journal Nature Communications
    Authors Dong Hanyang, et al.
    Applications Unspecified
    Reactivity
  • Post-translational changes in Phialophora verrucosa via lysine lactylation during prolonged presence in a patient with a CARD9-related immune disorder
    Year 2022
    Journal Frontiers in Immunology
    Authors Yinggai Song, et al.
    Applications Unspecified
    Reactivity
  • Protein Lactylation and Metabolic Regulation of the Zoonotic Parasite Toxoplasma gondii
    Year 2022
    Journal GENOMICS PROTEOMICS & BIOINFORMATICS
    Authors Deqi Yin, et al.
    Applications Unspecified
    Reactivity
  • Systematic identification of the lysine lactylation in the protozoan parasite Toxoplasma gondii
    Year 2022
    Journal Parasites & Vectors
    Authors Zhao Wei, et al.
    Applications Unspecified
    Reactivity
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