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Anti-L-Lactyllysine Rabbit pAb
Catalog Number: PTM-1401
$ 420

Clone Number: /

Host: Rabbit Clonality: Polyclonal

Applications： WB FC

Reactivity: All

Synonyms: Kla, L-la

Product Size
100 μl

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms Kla, L-la
UniProt ID

/

Immunogen L-lactylated lysine peptides
MW (kDa) Multiple
Specificity Anti-L-Lactyllysine Rabbit pAb detects proteins with L-lactylated lysine residues. This pan antibody recognizes L-lactylated lysine independent of its surrounding sequences.
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:1000 All
FC 1:50 Human
Properties
Purity Protein A and immunogen affinity purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Lactate, previously recognized solely as an energy source and metabolic byproduct, has now emerged as a crucial player in cancer biology, especially within the scope of the Warburg effect and its intricate interplay with various cellular processes, including angiogenesis, hypoxia, polarization of macrophages, and T cell activation. Recent investigations have unveiled an unprecedented function of lactate in the realm of histone modification, specifically through lysine L-lactylation, which exerts regulatory control over gene expression. The extent and dynamics of this modification are highly reliant on lactate levels within the cellular microenvironment and can be modulated through the introduction of extracellular lactate in cultured cells or the stimulation of intracellular glycolysis. The acetyltransferase p300 is responsible for introducing lysine L-lactylation, while Class I histone deacetylases (HDAC 1-3) have been identified as an eraser of the lactylation marks on histones.

Cellular location

/

Images
Dot Blot

Peptide amount: 4 ng, 20 ng, 100 ng
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 60 seconds
The list of peptides used in the experiment is provided in the table below:
WB

Lysates: (-): HeLa cells; (+): HeLa cells treated with 100 mM sodium lactate for 24 hours
Protein loading amount: 20 μg
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 60 seconds
Predicted band size: Multiple
Observed band size: Multiple

Lysates: Mouse kidney, mouse spleen
Protein loading amount: 20 μg
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 30 seconds
Predicted band size: Multiple
Observed band size: Multiple

Lysates: HeLa, N2a, BRL
Protein loading amount: 20 μg
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 30 seconds
Predicted band size: Multiple
Observed band size: Multiple
FC

Sample: HeLa cells
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% Triton X-100
Primary Ab dilution: 1:50
Secondary Ab: Goat Anti-Rabbit IgG
Unlabeled control: The cell without incubation with primary antibody and secondary antibody (black line).
Isotype control: Rabbit mAb IgG Isotype Control (blue line).
Description: The red line represents the positive signal observed with PTM-1401.

Research Use

For research use only, not for use in diagnostic procedures.

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References (33)
  • Hexokinase 2-mediated gene expression via histone lactylation is required for hepatic stellate cell activation and liver fibrosis
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    Journal Cell Metabolism
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    Applications Unspecified
    Reactivity
  • The diapause-like colorectal cancer cells induced by SMC4 attenuation are characterized by low proliferation and chemotherapy insensitivity
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  • ULK1-mediated metabolic reprogramming regulates Vps34 lipid kinase activity by its lactylation
    Year 2023
    Journal Science Advances
    Authors Mengshu Jia, et al.
    Applications Unspecified
    Reactivity
  • High-Intensity Interval Training Induces Protein Lactylation in Different Tissues of Mice with Specificity and Time Dependence
    Year 2023
    Journal Metabolites
    Authors Wenhua Huang, et al.
    Applications Unspecified
    Reactivity
  • Lactate dehydrogenase A mediated histone lactylation induced the pyroptosis through targeting HMGB1
    Year 2023
    Journal METABOLIC BRAIN DISEASE
    Authors Yao Xuan, et al.
    Applications Unspecified
    Reactivity
  • Positive feedback regulation of microglial glucose metabolism by histone H4 lysine 12 lactylation in Alzheimer’s disease
    Year 2022
    Journal Cell Metabolism
    Authors Rui-Yuan Pan, et al.
    Applications Unspecified
    Reactivity
  • ULK1-Mediated Metabolic Reprogramming Regulates Vps34 Lipid Kinase Activity by Its Lactylation
    Year 2022
    Journal MOLECULAR CELL
    Authors Yue Xiao, et al.
    Applications Unspecified
    Reactivity
  • Lactylation-driven METTL3-mediated RNA m6A modification promotes immunosuppression of tumor-infiltrating myeloid cells
    Year 2022
    Journal MOLECULAR CELL
    Authors Jia Xiong, et al.
    Applications Unspecified
    Reactivity
  • H3K18 lactylation marks tissue-specific active enhancers
    Year 2022
    Journal GENOME BIOLOGY
    Authors Galle Eva, et al.
    Applications Unspecified
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  • Nuclear Condensation of CDYL Links Histone Crotonylation and Cystogenesis in Autosomal Dominant Polycystic Kidney Disease
    Year 2022
    Journal JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
    Authors Lin Dang, et al.
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  • Class I histone deacetylases (HDAC1–3) are histone lysine delactylases
    Year 2022
    Journal Science Advances
    Authors Carlos Moreno-Yruela, et al.
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  • The global succinylation of SARS-CoV-2–infected host cells reveals drug targets
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    Journal PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
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  • YY1 Lactylation in Microglia Promotes Angiogenesis Through Transcription Activation-Mediated Upregulation of FGF2
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  • Autoregulation of H+/lactate efflux prevents monocarboxylate transport (MCT) inhibitors from reducing glycolytic lactic acid production
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  • Effect of lactylation on functional and structural properties of gluten
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    Authors Yu Wang, et al.
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  • Protein Lactylation and Metabolic Regulation of the Zoonotic Parasite Toxoplasma gondii
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    Authors Deqi Yin, et al.
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  • Single-Embryo Transcriptomic Atlas of Oxygen Response Reveals the Critical Role of HIF-1α in Prompting Major Zygotic Gene Activation
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    Applications Unspecified
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  • Hypoxia regulates fibrosis-related genes via histone lactylation in the placentas of patients with preeclampsia
    Year 2022
    Journal JOURNAL OF HYPERTENSION
    Authors Li Xiang, et al.
    Applications Unspecified
    Reactivity
  • LDHA promotes osteoblast differentiation through histone lactylation
    Year 2022
    Journal BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
    Authors Feige Nian, et al.
    Applications Unspecified
    Reactivity
  • Erythroid mitochondrial retention triggers myeloid-dependent type I interferon in human SLE
    Year 2021
    Journal CELL
    Authors Simone Caielli, et al.
    Applications Unspecified
    Reactivity
  • Lactate and IL6 define separable paths of inflammatory metabolic adaptation
    Year 2021
    Journal Science Advances
    Authors Stefanie Dichtl, et al.
    Applications Unspecified
    Reactivity
  • Histone lactylation drives oncogenesis by facilitating m6A reader protein YTHDF2 expression in ocular melanoma
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    Journal GENOME BIOLOGY
    Authors Yu Jie, et al.
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    Applications Unspecified
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    Year 2019
    Journal NATURE
    Authors Zhang Di, et al.
    Applications Unspecified
    Reactivity
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