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Recombinant
Anti-LSD1 Mouse mAb
Catalog Number: PTM-5960
$ 280

Clone Number: 11012

Host: Mouse Clonality: Recombinant Monoclonal

Applications: WB IHC-P

Reactivity: Human, Mouse, Rat

Synonyms: AOF2, KDM1, KIAA0601, KDM1A

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG1
Conjugate Unconjugated
Synonyms AOF2, KDM1, KIAA0601, KDM1A
UniProt ID

O60341

Immunogen
MW (kDa)
Specificity
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:1000 Human, Mouse, Rat
IHC-P 1:50 - 1:100 Human, Mouse
Properties
Purity Protein G purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Histone demethylase that can demethylate both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and Dimethylated (H3K4me2) H3K4me. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. Also acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in ANDR-containing complexes, which mediates phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag that prevents demethylation H3K4me, prevents H3K4me demethylase activity of KDM1A.

Cellular location

Nucleus

Images
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation: room temperature 2h
Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)
Lysate: HeLa, Jurkat, C2C12, Rat spleen
Protein loading quantity: 20 μg
Exposure time: 10 s
Predicted band size: 92 kDa
Observed band size: 110 kDa

IHC-P

Tissue: Human lung squamous carcinoma
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:100
Primary Ab incubation: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (Blue)
Description: The brown color represents the positive signal observed with PTM-5960

Tissue: Mouse stomach
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:100
Primary Ab incubation: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (Blue)
Description: The brown color represents the positive signal observed with PTM-5960

Research Use

For research use only, not for use in diagnostic procedures.

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    $ 280
    Recombinant
    Anti-LSD1 Mouse mAb
    Catalog #
    PTM-5938
    Host
    Mouse
    Clonality
    Recombinant Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Mouse
    Applications:
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P