Clone Number: 10822
Host: Rabbit Clonality: Recombinant Monoclonal
Applications： WB ICC/IF FC
Reactivity: Human
Synonyms: C6orf193, KDM1B
Shipping: Ambient temperature
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| Isotype | IgG |
| Conjugate | Unconjugated |
| Synonyms | C6orf193, KDM1B |
| UniProt ID | |
| Immunogen | |
| MW (kDa) | |
| Specificity |
| Applications | Dilution | Recommended Species |
|---|---|---|
| WB | 1:1000 - 1:2000 | Human |
| ICC/IF | 1:50 | Human |
| FC | 1:50 - 1:100 | Human |
| Purity | Protein A purified |
| Constituents | PBS, Glycerol, BSA |
| Storage | Store at -20°C. Avoid freeze/thaw cycles. |
| Stability | Stable for 12 months from date of receipt/reconstitution. |
Background
LSD2, also known as AOF1, is a Lysine (K)-specific demethylase that is encoded by the KDM1B gene in human. LSD2 demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. LSD2 demethylates both mono- and Dimethylated 'Lys-4' of histone H3. It acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed.
Cellular location
Nucleus
| WB | |
|---|---|
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Blocking buffer: 5% NFDM/TBST |
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Blocking buffer: 5% NFDM/TBST |
| ICC/IF | |
|---|---|
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Cell line: HeLa |
| FC | |
|---|---|
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Cell line: HeLa |
Research Use
For research use only, not for use in diagnostic procedures.
