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Recombinant Rabbit Monoclonal
Anti-Malonyl-Histone H3 (Lys23) Rabbit mAb
Catalog Number: PTM-911RM
$ 435

Clone Number: B1555/65-H1L5

Host: Rabbit Clonality: Recombinant Monoclonal

Applications： WB ICC/IF ChIP

Reactivity: Human, Mouse, Rat

Synonyms: H3K23ma

Product Size
100 μl

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms H3K23ma
UniProt ID

P68431

Immunogen
MW (kDa) 15
Specificity
Product Usage Information
Applications Dilution Recommended Species
Properties
Purity
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Cellular location

Images
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:5000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: (-): MCF-7, (+): MCF-7+ Sodium butyrate (50mM, 24hr) + Trichostatin A (500ng
ml, 4 hr)
Protein loading amount: 20 μg
Exposure time: 60 s
Predicted MW: 15 kDa
Observed MW: 15 kDa

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:5000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: (-):HeLa, (+):HeLa+Suramin
butyrate(5mM,7h)+Sodium malonate(30mM,16h)
Protein loading amount: 20 μg
Exposure time: 60 s
Predicted MW: 15 kDa
Observed MW: 15 kDa sodium
salt(40µM,7h)+Sodium

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:5000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: (-): C2C12, (+): C2C12+Suramin sodium salt(40µM,7h)+Sodium
butyrate(5mM,7h)+Sodium malonate(30mM,16h)
Protein loading amount: 20 μg
Exposure time: 60 s
Predicted MW: 15 kDa
Observed MW: 15 kDa

Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:5000
Primary ab incubation condition: 2 hours at room temperature
Lysate: (-): BRL, (+): BRL + Suramin sodium (40µM, 7h) + Sodium butyrate (5mM, 7h)
+ Sodium malonate (30mM, 16h)
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 15 kDa
Observed MW: 15 kDa
ChIP

Cell type: MCF-7+Sodium butyrate (50 mM, 24 hr)+trichostatin A (500 ng/ml, 4 hr)
Cross-linking conditions: no cross-linking
Amount of chromatin per IP: 5×10
6
cells
Amount of Ab per IP: 6 μg
Beads type and amount per IP: 50 μL of Protein A MagBeads
Comment:
The ChIP was performed with 6 μg of normal Rabbit IgG as a negative control. Real
time quantitative PCR was performed on immunoprecipitated DNA using primers specific
for the human GAPDH CDS region, RPL30, LDHA-promoter, LDHA and TuBBP10. Data
are presented as enrichment of each sample relative to total amount of input chromatin
at each amplicon.
ICC/IF

Cell line: HeLa+ +Suramin sodium salt (40µM,7h)+Sodium butyrate
(5mM,7h)+Sodium malonate (30mM,16h)
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% TritonX-100
Primary ab dilution: 1:500
Primary incubation condition: 4°C overnight
Secondary ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (Blue)
Comment: Color red is the positive signal for PTM-911RM

Research Use

For research use only, not for use in diagnostic procedures.

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