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Anti-Malonyllysine Antibody Conjugated Agarose Beads
Catalog Number: PTM-904
$ 1,920

Clone Number: /

Host: / Clonality: /

Applications： IAP

Reactivity: All

Synonyms: Kmal

Product Size
500 μl
Quantity

Shipping: Cold packs / dry ice

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Agarose
Synonyms Kmal
UniProt ID

/

Immunogen Malonylated lysine peptides
MW (kDa) Multiple
Specificity With a mixture of high-quality rabbit- and mouse-derived anti-malonyllysine antibodies cross-linked, this Anti-Malonyllysine Antibody Conjugated Agarose Beads present extraordinary capability to specifically capture peptides/proteins bearing phosphotyrosine residues but do not cross-react with the peptides/proteins bearing structurally similar succinyllysine, propionyllysine, or butyryllysine residues. This product has been well utilized to affinity purify peptides for global proteomic screening of lysine malonylation.
Product Usage Information
Applications Dilution Recommended Species
IAP 40 μl beads per 2 mg peptides All
Properties
Purity /
Constituents Antibody conjugated agarose beads are supplied as a 50% slurry containing 66% glycerol and 4 mg/ml immobilized antibody.
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt.
Target Information

Background

Malonylation of lysine is a newly identified reversible modification controlling protein activity. Sirt5, a regulatory enzyme for lysine malonylation, can catalyze lysine demalonylation reactions in vivo and in vitro. Through integrated proteomic approaches and biochemistry analysis, it has been found that lysine malonylation is widely distributed acrossvarious histones and non-histone substrates. Lysine malonylation may provide a new paradigm in cellular regulation, such as energy metabolism in diverse organisms.

Cellular location

/

Images
Dot Blot

Dot blot analysis of Anti-Malonyllysine Rabbit pAb (PTM-901) on different acylated proteins and peptides.
Peptides/Protein amount: 100 ng
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 60 seconds
The list of peptides/proteins used in the experiment is provided below.
Dot 1: Malonylated peptide library. Dot 2: Malonylated BSA.
Dot 3: Succinylated peptide library. Dot 4: Succinylated BSA.
Dot 5: Propionylated peptide library. Dot 6: Propionylated BSA.
Dot 7: Butyrylated peptide library. Dot 8: Butyrylated BSA.
Dot 9: Unmodified peptide library. Dot 10: BSA.

Research Use

For research use only, not for use in diagnostic procedures.

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    Catalog #
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    Host
    Rabbit
    Clonality
    Recombinant Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Mouse, Rat
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
References (19)
  • Systematic analysis of lysine malonylation in Streptococcus mutans
    Year 2022
    Journal Frontiers in Cellular and Infection Microbiology
    Authors Li Zhengyi, et al.
    Applications Unspecified
    Reactivity
  • Global profiling of protein lysine malonylation in Toxoplasma gondii strains of different virulence and genetic backgrounds
    Year 2022
    Journal PLoS Neglected Tropical Diseases
    Authors Lan-Bi Nie, et al.
    Applications Unspecified
    Reactivity
  • Global landscape of lysine acylomes in Bacillus subtilis
    Year 2022
    Journal Journal of Proteomics
    Authors Mingya Zhang, et al.
    Applications Unspecified
    Reactivity
  • Global Identification and Systematic Analysis of Lysine Malonylation in Maize (Zea mays L.)
    Year 2021
    Journal Frontiers in Plant Science
    Authors Xu Min, et al.
    Applications Unspecified
    Reactivity
  • Antimicrobial Peptide BCp12 Inhibits Staphylococcus aureus Growth by Altering Lysine Malonylation Levels in the Arginine Synthesis Pathway
    Year 2021
    Journal JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
    Authors Yanan Shi, et al.
    Applications Unspecified
    Reactivity
  • Malonyl-proteome profiles of Staphylococcus aureus reveal lysine malonylation modification in enzymes involved in energy metabolism
    Year 2021
    Journal Proteome Science
    Authors Shi Yanan, et al.
    Applications Unspecified
    Reactivity
  • Global Profiling of Protein Lysine Malonylation in Eucommia ulmoides Leaves Reveals Its Role in Photosynthesis and Metabolism
    Year 2021
    Journal PLANT MOLECULAR BIOLOGY REPORTER
    Authors Yao Xinzhuan, et al.
    Applications Unspecified
    Reactivity
  • Global Proteomic Analysis of Lysine Malonylation in Toxoplasma gondii
    Year 2020
    Journal Frontiers in Microbiology
    Authors Nie Lan-Bi, et al.
    Applications Unspecified
    Reactivity
  • Malonylation of GAPDH is an inflammatory signal in macrophages
    Year 2019
    Journal Nature Communications
    Authors Galván-Peña Silvia, et al.
    Applications Unspecified
    Reactivity
  • SIRT5 deacylates metabolism-related proteins and attenuates hepatic steatosis in ob/ob mice
    Year 2018
    Journal EBioMedicine
    Authors Yipeng Du, et al.
    Applications Unspecified
    Reactivity
  • Systematic analysis of the lysine malonylome in common wheat
    Year 2018
    Journal BMC GENOMICS
    Authors Liu Jiabin, et al.
    Applications Unspecified
    Reactivity
  • Malonylome Analysis Reveals the Involvement of Lysine Malonylation in Metabolism and Photosynthesis in Cyanobacteria
    Year 2017
    Journal JOURNAL OF PROTEOME RESEARCH
    Authors Yanyan Ma, et al.
    Applications Unspecified
    Reactivity
  • Malonylome analysis in developing rice (Oryza sativa) seeds suggesting that protein lysine malonylation is well-conserved and overlaps with acetylation and succinylation substantially
    Year 2017
    Journal Journal of Proteomics
    Authors Hana Mujahid, et al.
    Applications Unspecified
    Reactivity
  • SIRT7 is a histone desuccinylase that functionally links to chromatin compaction and genome stability
    Year 2016
    Journal Nature Communications
    Authors Li Lei, et al.
    Applications Unspecified
    Reactivity
  • Lysine Malonylome May Affect the Central Metabolism and Erythromycin Biosynthesis Pathway in Saccharopolyspora erythraea
    Year 2016
    Journal JOURNAL OF PROTEOME RESEARCH
    Authors Jun-Yu Xu, et al.
    Applications Unspecified
    Reactivity
  • Prediction of Protein Lysine Acylation by Integrating Primary Sequence Information with Multiple Functional Features
    Year 2016
    Journal JOURNAL OF PROTEOME RESEARCH
    Authors Yipeng Du, et al.
    Applications Unspecified
    Reactivity
  • Global Profiling of Protein Lysine Malonylation in Escherichia coli Reveals Its Role in Energy Metabolism
    Year 2016
    Journal JOURNAL OF PROTEOME RESEARCH
    Authors Lili Qian, et al.
    Applications Unspecified
    Reactivity
  • Malonylome analysis of rhizobacterium Bacillus amyloliquefaciens FZB42 reveals involvement of lysine malonylation in polyketide synthesis and plant-bacteria interactions
    Year 2016
    Journal Journal of Proteomics
    Authors Ben Fan, et al.
    Applications Unspecified
    Reactivity
  • Proteomic and Biochemical Studies of Lysine Malonylation Suggest Its Malonic Aciduria-associated Regulatory Role in Mitochondrial Function and Fatty Acid Oxidation[S]
    Year 2015
    Journal MOLECULAR & CELLULAR PROTEOMICS
    Authors Gozde Colak, et al.
    Applications Unspecified
    Reactivity
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