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Anti-Malonyllysine Mouse mAb
Catalog Number: PTM-902
$ 380

Clone Number: /

Host: Mouse Clonality: Monoclonal

Applications： WB

Reactivity: Human, Mouse, Rat

Synonyms: Kmal

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms Kmal
UniProt ID

/

Immunogen Malonylated lysine peptides
MW (kDa) Multiple
Specificity
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:1000 All
Properties
Purity Protein G and immunogen affinity purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Malonylation of lysine is a newly identified reversible modification controlling protein activity. Sirt5, a regulatory enzyme for lysine malonylation, can catalyze lysine demalonylation reactions in vivo and in vitro. Through integrated proteomic approaches and biochemistry analysis, it has been found that lysine malonylation is widely distributed acrossvarious histones and non-histone substrates. Lysine malonylation may provide a new paradigm in cellular regulation, such as energy metabolism in diverse organisms.

Cellular location

/

Images
Dot Blot

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)
Immunogen peptide quantity: 4 ng, 20 ng, 100 ng
Exposure time: 60 seconds
The list of peptides is included in the table below.
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)
Lysate: JK, 293T
Protein loading quantity: 20 μg
Exposure time: 60 seconds
Predicted band size: Multiple kDa
Observed band size: Multiple kDa

Research Use

For research use only, not for use in diagnostic procedures.

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References (13)
  • SIRT5 alleviates liver ischemia/reperfusion injury by regulating mitochondrial succinylation and oxidative stress
    Year 2023
    Journal ANTIOXIDANTS & REDOX SIGNALING
    Authors Prof. Jihong Yao, et al.
    Applications Unspecified
    Reactivity
  • The Enzyme Lysine Malonylation of Calvin Cycle and Gluconeogenesis Regulated Glycometabolism in Nostoc flagelliforme to Adapt to Drought Stress
    Year 2023
    Journal INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
    Authors Meng Wang, et al.
    Applications Unspecified
    Reactivity
  • Global proteomic analysis reveals lysine succinylation contributes to the pathogenesis of aortic aneurysm and dissection
    Year 2023
    Journal Journal of Proteomics
    Authors Hongwei Zhang, et al.
    Applications Unspecified
    Reactivity
  • Tandem mass tag-based quantitative proteomic analysis identification of succinylation related proteins in pathogenesis of thoracic aortic aneurysm and aortic dissection
    Year 2023
    Journal PeerJ
    Authors Yu Zhang, et al.
    Applications Unspecified
    Reactivity
  • TFAM loss induces nuclear actin assembly upon mDia2 malonylation to promote liver cancer metastasis
    Year 2022
    Journal EMBO JOURNAL
    Authors Qichao Huang, et al.
    Applications Unspecified
    Reactivity
  • GABA regulates IL-1β production in macrophages
    Year 2022
    Journal Cell Reports
    Authors Jian Fu, et al.
    Applications Unspecified
    Reactivity
  • Systematic analysis of lysine malonylation in Streptococcus mutans
    Year 2022
    Journal Frontiers in Cellular and Infection Microbiology
    Authors Li Zhengyi, et al.
    Applications Unspecified
    Reactivity
  • Cold Atmospheric Plasma Conveys Selectivity Against Hepatocellular Carcinoma Cells via Triggering EGFR(Tyr1068)-Mediated Autophagy
    Year 2022
    Journal Frontiers in Oncology
    Authors Danjun Wang, et al.
    Applications Unspecified
    Reactivity
  • Integrated insights into the mechanisms underlying sepsis-induced myocardial depression using a quantitative global proteomic analysis
    Year 2022
    Journal Journal of Proteomics
    Authors Ni Yang, et al.
    Applications Unspecified
    Reactivity
  • The decrotonylase FoSir5 facilitates mitochondrial metabolic state switching in conidial germination of Fusarium oxysporum
    Year 2021
    Journal eLife
    Authors Ning Zhang, et al.
    Applications Unspecified
    Reactivity
  • Global Identification and Systematic Analysis of Lysine Malonylation in Maize (Zea mays L.)
    Year 2021
    Journal Frontiers in Plant Science
    Authors Xu Min, et al.
    Applications Unspecified
    Reactivity
  • Antimicrobial Peptide BCp12 Inhibits Staphylococcus aureus Growth by Altering Lysine Malonylation Levels in the Arginine Synthesis Pathway
    Year 2021
    Journal JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
    Authors Yanan Shi, et al.
    Applications Unspecified
    Reactivity
  • Malonyl-proteome profiles of Staphylococcus aureus reveal lysine malonylation modification in enzymes involved in energy metabolism
    Year 2021
    Journal Proteome Science
    Authors Shi Yanan, et al.
    Applications Unspecified
    Reactivity
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