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Anti-Malonyllysine Rabbit pAb
Catalog Number: PTM-901
$ 420

Clone Number: /

Host: Rabbit Clonality: Polyclonal

Applications: WB ICC/IF

Reactivity: All

Synonyms: Kmal

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms Kmal
UniProt ID

/

Immunogen Malonylated lysine peptides
MW (kDa) Multiple
Specificity Anti-Malonyllysine Rabbit pAb detects proteins with malonylated lysine residues. This pan antibody recognizes malonylated lysine independent of its surrounding sequences.
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:1000 All
ICC/IF 1:50 Human
Properties
Purity Protein A and immunogen affinity purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Malonylation of lysine is a newly identified reversible modification controlling protein activity. Sirt5, a regulatory enzyme for lysine malonylation, can catalyze lysine demalonylation reactions in vivo and in vitro. Through integrated proteomic approaches and biochemistry analysis, it has been found that lysine malonylation is widely distributed across various histones and non-histone substrates. Lysine malonylation may provide a new paradigm in cellular regulation, such as energy metabolism in diverse organisms.

Cellular location

/

Images
Dot Blot

Peptides/Protein amount: 100 ng
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 60 seconds
The list of peptides/proteins used in the experiment is provided below.
Dot 1: Malonylated peptide library. Dot 2: Malonylated BSA.
Dot 3: Succinylated peptide library. Dot 4: Succinylated BSA.
Dot 5: Propionylated peptide library. Dot 6: Propionylated BSA.
Dot 7: Butyrylated peptide library. Dot 8: Butyrylated BSA.
Dot 9: Unmodified peptide library. Dot 10: BSA.

WB

Lysates: (-) HeLa; (+) HeLa cells treated with 20 mM sodium malonylate for 72 hours
Protein loading amount: 20 μg
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 60 seconds
Predicted band size: Multiple
Observed band size: Multiple

ICC/IF

Samples: (A) HeLa cells; (B) HeLa cells + sodium malonylate (20 mM, 72 hours)
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% Triton X-100
Primary Ab dilution: 1:50
Primary Ab incubation condition: 4°C overnight
Secondary Ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (blue)
Description: The green color represents the positive signal observed with PTM-901.

Research Use

For research use only, not for use in diagnostic procedures.

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  • Lysine 2-hydroxyisobutyrylation of NAT10 promotes cancer metastasis in an ac4C-dependent manner
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  • The Deacylase Sirtuin 5 Reduces Malonylation in Non-mitochondrial Metabolic Pathways in Diabetic Kidney Disease
    Year 2023
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  • Sirtuin5 protects colorectal cancer from DNA damage by keeping nucleotide availability
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  • Global profiling of protein lysine malonylation in mouse cardiac hypertrophy
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  • The Acyl-CoA Specificity of Human Lysine Acetyltransferase KAT2A
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  • Dihydroartemisinin ameliorates retinal vascular dysfunction in diabetes mellitus via the FASN/Kmal-mTOR/SREBP1 feedback loop
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  • Loss of the mitochondrial phosphate carrier SLC25A3 induces remodeling of the cardiac mitochondrial protein acylome
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    Authors Jessica N. Peoples, et al.
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  • Synthesis and Evaluation of a Stable Isostere of Malonyllysine**
    Year 2021
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    Authors Yihang Jing, et al.
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  • Dysregulation of the Sirt5/IDH2 axis contributes to sunitinib resistance in human renal cancer cells
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  • Skeletal muscle of torpid Richardson's ground squirrels (Urocitellus richardsonii) exhibits a less active form of citrate synthase associated with lowered lysine succinylation
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    Authors Liang Shi, et al.
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    Authors Chao Wang, et al.
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    Year 2018
    Journal Scientific Reports
    Authors Tanabe Kana, et al.
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    Reactivity
  • Purification of carbamoyl phosphate synthetase 1 (CPS1) from wood frog (Rana sylvatica) liver and its regulation in response to ice-nucleation and subsequent whole-body freezing
    Year 2018
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    Authors Green Stuart R, et al.
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  • Synthetic Chromatin Acylation by an Artificial Catalyst System
    Year 2017
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  • A Class of Reactive Acyl-CoA Species Reveals the Non-enzymatic Origins of Protein Acylation
    Year 2017
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    Authors Gregory R. Wagner, et al.
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  • Characterization of histone acylations links chromatin modifications with metabolism
    Year 2017
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    Authors Simithy Johayra, et al.
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  • Malonylome Analysis Reveals the Involvement of Lysine Malonylation in Metabolism and Photosynthesis in Cyanobacteria
    Year 2017
    Journal JOURNAL OF PROTEOME RESEARCH
    Authors Yanyan Ma, et al.
    Applications Unspecified
    Reactivity
  • Malonylome analysis in developing rice (Oryza sativa) seeds suggesting that protein lysine malonylation is well-conserved and overlaps with acetylation and succinylation substantially
    Year 2017
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    Authors Hana Mujahid, et al.
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  • NAMPT-Mediated NAD+ Biosynthesis Is Essential for Vision In Mice
    Year 2016
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    Authors Jonathan B. Lin, et al.
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  • SIRT5 promotes IDH2 desuccinylation and G6PD deglutarylation to enhance cellular antioxidant defense
    Year 2016
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    Reactivity
  • Lysine Malonylome May Affect the Central Metabolism and Erythromycin Biosynthesis Pathway in Saccharopolyspora erythraea
    Year 2016
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    Authors Jun-Yu Xu, et al.
    Applications Unspecified
    Reactivity
  • Prediction of Protein Lysine Acylation by Integrating Primary Sequence Information with Multiple Functional Features
    Year 2016
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    Authors Yipeng Du, et al.
    Applications Unspecified
    Reactivity
  • Lysine Malonylation Is Elevated in Type 2 Diabetic Mouse Models and Enriched in Metabolic Associated Proteins *
    Year 2015
    Journal MOLECULAR & CELLULAR PROTEOMICS
    Authors Yipeng Du, et al.
    Applications Unspecified
    Reactivity
  • Aberrant protein acylation is a common observation in inborn errors of acyl-CoA metabolism
    Year 2014
    Journal JOURNAL OF INHERITED METABOLIC DISEASE
    Authors Pougovkina, et al.
    Applications Unspecified
    Reactivity