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Recombinant
Anti-MBNL2 Mouse mAb
Catalog Number: PTM-6592
$ 280

Clone Number: JMMR-3329

Host: Mouse Clonality: Recombinant Monoclonal

Applications： WB IHC-P

Reactivity: Human, Mouse, Rat

Synonyms: MBNL2, MBLL, MBLL39, MLP1

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG2b/kappa
Conjugate Unconjugated
Synonyms MBNL2, MBLL, MBLL39, MLP1
UniProt ID

Q5VZF2

Immunogen
MW (kDa)
Specificity
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:1000 Human, Mouse, Rat
IHC-P 1:50 - 1:100 Human, Mouse, Rat
Properties
Purity Protein A purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

MBNL2 mediates pre-mRNA alternative splicing regulation. It acts either as activator or repressor of splicing on specific pre-mRNA targets. It inhibits cardiac troponin-T (TNNT2) pre-mRNA exon inclusion but induces insulin receptor (IR) pre-mRNA exon inclusion in muscle. It antagonizes the alternative splicing activity pattern of CELF proteins. It is an RNA-binding protein that binds to 5'ACACCC-3' core sequence within the 3'UTR of ITGA3. It binds to CUG triplet repeat expansion in myotonic dystrophy muscle cells by sequestering the target RNAs.

Cellular location

Nucleus, Cytoplasm

Images
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Mouse IgGH&L(HRP Conjugate)
Lysate: 1: HeLa, 2: HepG2, 3: Mouse brain, 4: Rat liver
Protein loading quantity: 20 μg
Exposure time: 60 seconds
Predicted band size: 39, 41 kDa
Observed band size: 39, 41 kDa
IHC-P

Tissue: Human cerebrum
Section type: Formalin-fixed & Paraffin-embedded section
Retrieval method: High temperature and highpressure
Retrieval buffer: Tris/EDTA buffer, pH9.0
Primary Ab dilution: 1:100
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (Blue)
Description: The brown color represents the positive signal observed with PTM-6592

Tissue: Human ovary
Section type: Formalin-fixed & Paraffin-embedded section
Retrieval method: High temperature and highpressure
Retrieval buffer: Tris/EDTA buffer, pH9.0
Primary Ab dilution: 1:100
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (Blue)
Description: The brown color represents the positive signal observed with PTM-6592

Tissue: Mouse liver
Section type: Formalin-fixed & Paraffin-embedded section
Retrieval method: High temperature and highpressure
Retrieval buffer: Tris/EDTA buffer, pH9.0
Primary Ab dilution: 1:100
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (Blue)
Description: The brown color represents the positive signal observed with PTM-6592

Tissue: Rat cerebrum
Section type: Formalin-fixed & Paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:100
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (Blue)
Description: The brown color represents the positive signal observed with PTM-6592

Tissue: Rat heart
Section type: Formalin-fixed & Paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:100
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (Blue)
Description: The brown color represents the positive signal observed with PTM-6592

Tissue: Rat kidney
Section type: Formalin-fixed & Paraffin-embedded section
Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (Blue)
Description: The brown color represents the positive signal observed with PTM-6592

Research Use

For research use only, not for use in diagnostic procedures.

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