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Recombinant
Anti-MED30 Mouse mAb
Catalog Number: PTM-6459
$ 285

Clone Number: JMMR-2715

Host: Mouse Clonality: Recombinant Monoclonal

Applications: WB

Reactivity: Human, Mouse

Synonyms: Trap25, THRAP6

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG2a
Conjugate Unconjugated
Synonyms Trap25, THRAP6
UniProt ID

Q96HR3

Immunogen
MW (kDa) 20
Specificity
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:1000 Human, Mouse
Properties
Purity Protein A purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Mediator of RNA polymerase II transcription subunit 30 (MED30) is an enzyme, encoded by the MED30 gene in human. It represents subunit Med30 of the Mediator complex and is metazoan-specific with no homologues in yeasts. It is a coactivator involved in the transcription regulation of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery.

Cellular location

Nucleus

Images
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation: 2 hours at room temperature
Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)
Lysate: 1: HEK-293T, 2: Jurkat, 3: Neuro-2a
Protein loading quantity: 20 μg
Exposure time: 30 seconds
Predicted band size: 20 kDa
Observed band size: 20 kDa

Research Use

For research use only, not for use in diagnostic procedures.