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Recombinant Rabbit Monoclonal
Anti-Monomethyl-Histone H3 (Lys36) Rabbit mAb
Catalog Number: PTM-623RM
$ 330

Clone Number: 12H1L2

Host: Rabbit Clonality: Recombinant Monoclonal

Applications: WB IHC-P ICC/IF IP

Reactivity: Human, Mouse, Rat

Synonyms: H3K36me1

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms H3K36me1
UniProt ID

P68431

Immunogen
MW (kDa)
Specificity
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:2000 Human, Mouse, Rat
IHC-P 1:100 - 1:500 Human, Mouse, Rat
ICC/IF 1:50 Human
IP 1:50 - 1:100 Human
Properties
Purity Protein A purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Histone post-translational modifications (PTMs) are key mechanisms of epigenetics that modulate chromatin structures, termed as “histone code”. The PTMs on histone including acetylation, methylation, phosphorylation and novel acylations directly affect the accessibility of chromatin to transcription factors and other epigenetic regulators, altering genome stability, gene transcription, etc. Histone methylation occurs primarily at lysine and arginine residues on the amino terminal of core histones. Methylation of histones can either increase or decrease transcription of genes, depending on which amino acids (Lys or Arg) in the histones are methylated and how many methyl groups are attached (mono-, di-, Trimethylation on Lys, mono-di-symmetric/asymmetric methylation on Arg). Mostly, lysine methylation occurs primarily on histone H3 Lys4, 9, 27, 36, 79 and H4 Lys20, while Arginine methylation occurs primarily on histone H3 Arg2, 8, 17, 26 and H4 Arg3. Histone methylases (HMTs) and histone demethylases (HDMs) are major regulating factors.

Cellular location

Nucleus

Images
Dot Blot

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1: 2000
Primary Ab incubation condition: 2 hours at room temperature
Immunogen peptide quantity: 4 ng, 16 ng, 64 ng
Exposure time: 30 seconds
The list of peptides is included in the table below.

WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1: 2000
Primary Ab incubation condition: 2 hours at room temperature
Lysate: HeLa Protein loading quantity: 20 μg
Exposure time: 1 s
Predicted band size: 17 kDa
Observed band size: 17 kDa

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1: 2000
Primary Ab incubation condition: 2 hours at room temperature
Lysate: BRL, Mouse liver, Mouse kidney, Recombinant Histone H3
Protein loading quantity: 20 μg/20 ng(r H3)
Exposure time: 1 s
Predicted band size: 17 kDa
Observed band size: 17 kDa

IHC-P

Tissue: Human lung adenocarcinoma
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1: 500
Primary Ab incubation condition: 1 hour at room temperature
Counter stain: Hematoxylin
Description: The brown color represents the positive signal observed with PTM-623RM

Tissue: Mouse liver
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1: 500
Primary Ab incubation condition: 1 hour at room temperature
Counter stain: Hematoxylin
Description: The brown color represents the positive signal observed with PTM-623RM

Tissue: Rat kidney
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1: 500
Primary Ab incubation condition: 1 hour at room temperature
Counter stain: Hematoxylin
Description: The brown color represents the positive signal observed with PTM-623RM

ICC/IF

Cell line: HeLa
Fixative: 100% Ice-cold methanol
Permeabilization: 0.1% TritonX-100
Primary Ab dilution: 1:50
Primary incubation condition: 4°C over night
Nuclear counter stain: DAPI (Blue)
Counter stain: Tubulin (Red)
Description: The green color represents the positive signal observed with PTM-623RM

IP

IP of Jurkat cells extracts
IP Ab incubation condition: PTM-623RM, 4°C overnight, 1:100 dilution
WB Primary Ab incubation condition: PTM-623RM, room temperature 2h, 1:2000
dilution
Secondary Ab: Anti-Rabbit IgG for IP (HRP Conjugate)
Blocking buffer and concentration: 5% NFDM/TBST
Diluting buffer and concentration: 5% NFDM/TBST
1: 5% Input
2: IP with PTM-623RM
3: IP with Rabbit monoclonal IgG Isotype
Predicted band size: 15 kDa
Observed band size: 15 kDa
Exposure time: 60 s

Research Use

For research use only, not for use in diagnostic procedures.

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    $ 340
    Anti-Monomethyl-Histone H3 (Lys36) Rabbit pAb
    Catalog #
    PTM-623
    Host
    Rabbit
    Clonality
    Polyclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Mouse, Rat
    Applications:
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P