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Recombinant Rabbit Monoclonal
Anti-Monomethyl-Histone H3 (Lys79) Rabbit mAb
Catalog Number: PTM-628RM
$ 320

Clone Number: PA-O0802

Host: Rabbit Clonality: Recombinant Monoclonal

Applications: WB IP ChIP

Reactivity: Human, Mouse, Rat

Synonyms: H3K79me1

Product Size
100μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms H3K79me1
UniProt ID

P68431

Immunogen
MW (kDa) 15
Specificity Anti-Mono-Methyl-Histone H3 (Lys79) Rabbit mAb detects histone H3 only when it is Methylated at Lys79.
Product Usage Information
Applications Dilution Recommended Species
WB 1:1000-1:2000 Human, Mouse, Rat
IP 1:50-1:100 Human
ChIP 6 μg per 100 μg chromatin Human
Properties
Purity Protein A
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Histone post-translational modifications (PTMs) are key mechanisms of epigenetics that modulate chromatin structures, termed as “histone code”. The PTMs on histone including acetylation, methylation, phosphorylation and novel acylations directly affect the accessibility of chromatin to transcription factors and other epigenetic regulators, altering genome stability, gene transcription, etc. Histone methylation occurs primarily at lysine and arginine residues on the amino terminal of core histones. Methylation of histones can either increase or decrease transcription of genes, depending on which amino acids (Lys or Arg) in the histones are methylated and how many methyl groups are attached (mono-, di-, trimethylation on Lys, mono-di-symmetric/asymmetric methylation on Arg). Mostly, lysine methylation occurs primarily on histone H3 Lys4, 9, 27, 36, 79 and H4 Lys20, while Arginine methylation occurs primarily on histone H3 Arg2, 8, 17, 26 and H4 Arg3. Histone methylases (HMTs) and histone demethylases (HDMs) are major regulating factors.

Cellular location

Nucleus

Images
Dot Blot

Blocking buffer: 5% NFDM/TBST

Primary ab dilution: 1:2000

Primary ab incubation condition: 2 hours at room temperature

Secondary ab: Goat Anti-rabbit IgG H&L (HRP)

Peptide amount: 1-2: 1 ng, 4 ng, 16 ng, 64ng; 3-5:64ng

Exposure time: 60 s

The list of peptides is included below:

Lane 1 H3K79me1. Lane 2 H3K79me2.

Lane 3 H3K79me3. Lane 4 H3K79ac.

Lane 5 H3K79un.

WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: 1: HeLa, 2: Neuro-2a, 3: BRL, 4: Recombinant Histone H3
Protein loading amount: 20 μg
Exposure time: 30 s
Predicted MW: 17 kDa
Observed MW: 17 kDa

IP

IP of HeLa cells extracts
IP Ab incubation condition: PTM-628RM, 4°C overnight, 1:100 dilution
WB primary Ab incubation condition: PTM-628RM, room temperature 2h, 1: 1000 dilution
Secondary Ab: Anti-Rabbit IgG for IP (HRP)
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
1: 2.5% Input
2: IP with PTM-628RM
3: IP with Rabbit monoclonal IgG Isotype
Observed MW: 17 kDa
Exposure time: 10 s

ChIP

Cell type: HeLa
Cross-linking conditions: no cross-linking
Amount of chromatin per IP: 100 μg
Amount of Ab per IP: 6 ug
Beads type and amount per IP: 50 μl of Protein A MagBeads
Comment: The ChIP was performed with 6 μg of normal Rabbit IgG as a negative control. Real time quantitative PCR was performed on immunoprecipitated DNA using primers specific for the human GAPDH-CDS. Data are presented as enrichment of each sample relative to total amount of input chromatin at each amplicon.

Research Use

For research use only, not for use in diagnostic procedures.