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Anti-Monomethyl-Histone H3 (Lys9) Mouse mAb
Catalog Number: PTM-643
This item is discontinued.
Please see alternative products below.

Clone Number: /

Host: Mouse Clonality: Monoclonal

Applications： WB IHC-P IP

Reactivity: Human, Mouse, Rat

Synonyms: H3K9me1

Product Size
100 μl

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

Alternative Products
  • View More Images（5）
    $ 340
    Anti-Monomethyl-Histone H3 (Lys9) Rabbit pAb
    Catalog #
    PTM-614
    Host
    Rabbit
    Clonality
    Polyclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Mouse, Rat, Monkey, Pharbitis
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
  • View More Images（2）
    $ 330
    Recombinant Rabbit Monoclonal
    Anti-Monomethyl-Histone H3 (Lys9) Rabbit mAb
    Catalog #
    PTM-7287
    Host
    Rabbit
    Clonality
    Recombinant Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Mouse, Rat
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
General Information
Isotype IgG
Conjugate Unconjugated
Synonyms H3K9me1
UniProt ID

P68431

Immunogen
MW (kDa)
Specificity
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:2000 Human, Mouse, Rat, African green monkey
IHC-P 1:200 - 1:1000 Human, Mouse
IP 1:12.5 Mouse
Properties
Purity Protein G and immunogen affinity purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Histone post-translational modifications (PTMs) are key mechanisms of epigenetics that modulate chromatin structures, termed as “histone code”. The PTMs on histone including acetylation, methylation, phosphorylation and novel acylations directly affect the accessibility of chromatin to transcription factors and other epigenetic regulators, altering genome stability, gene transcription, etc. Histone methylation occurs primarily at lysine and arginine residues on the amino terminal of core histones. Methylation of histones can either increase or decrease transcription of genes, depending on which amino acids (Lys or Arg) in the histones are methylated and how many methyl groups are attached (mono-, di-, Trimethylation on Lys, mono-di-symmetric/asymmetric methylation on Arg). Mostly, lysine methylation occurs primarily on histone H3 Lys4, 9, 27, 36, 79 and H4 Lys20, while Arginine methylation occurs primarily on histone H3 Arg2, 8, 17, 26 and H4 Arg3. Histone methylases (HMTs) and histone demethylases (HDMs) are major regulating factors.

Cellular location

Nucleus

Images
Dot Blot

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)
Immunogen peptide quantity: 1 ng, 4 ng, 16 ng
Exposure time: 30 s
The list of peptides is included in the table below.
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)
Lysate: MCF-7, C2C12, COS
Protein loading quantity: 20 μg
Exposure time: 30 s
Predicted band size: 30 kDa
Observed band size: 30 kDa
IHC-P

Tissue: Human spleen
Section type: Formalin-fixed & Paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (Blue)
Description: The brown color represents the positive signal observed with PTM-643

Tissue: Mouse brain
Section type: Formalin-fixed & Paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (Blue)
Description: The brown color represents the positive signal observed with PTM-643
IP

IP of Mouse liver extracts
IP Ab incubation condition: PTM-643, 4°C overnight, 1:12.5, 1:50 dilution
WB Primary Ab incubation condition: PTM_x0002_643, room temperature 2h, 1:1000 dilution
Secondary Ab: Anti-Mouse IgG for IP (HRP Conjugate)
Blocking buffer and concentration: 5% NFDM/TBST
Diluting buffer and concentration: 5% NFDM/TBST
Lane 1: 5% Input
Lane 2: IP with PTM-643 (1:12.5)
Lane 3: IP with PTM-643 (1:50)
Observed band size: 17 kDa
Exposure time: 30 s

Research Use

For research use only, not for use in diagnostic procedures.

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References (1)
  • Functional characterization of a special dicistronic transcription unit encoding histone methyltransferase su(var)3-9 and translation regulator eIF2γ in Tribolium castaneum
    Year 2020
    Journal BIOCHEMICAL JOURNAL
    Authors Song Xiaowen, et al.
    Applications Unspecified
    Reactivity
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