Clone Number: JRMR-10037-23
Host: Rabbit Clonality: Recombinant Monoclonal
Applications： WB ICC/IF IP
Reactivity: /
Synonyms: /
Shipping: Ambient temperature
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| Isotype | IgG |
| Conjugate | Unconjugated |
| Synonyms | / |
| UniProt ID | |
| Immunogen | Synthetic peptide corresponding to the Myc tag sequence: EQKLISEEDL |
| MW (kDa) | / |
| Specificity | Anti-Myc Tag Rabbit mAb recognizes the Myc tag sequence (EQKLISEEDL) and is designed to detect exogeneously expressed Myc-tagged proteins. |
| Applications | Dilution | Recommended Species |
|---|---|---|
| WB | 1:500 - 1:2000 | / |
| ICC/IF | 1:500 - 1:2000 | / |
| IP | 1:50 | / |
| Purity | Protein A purified |
| Constituents | PBS, Glycerol, BSA |
| Storage | Store at -20°C. Avoid freeze/thaw cycles. |
| Stability | Stable for 12 months from date of receipt/reconstitution. |
Background
The Myc tag offers a reliable method for localizing gene products across various cell types, studying protein and protein complex topology, and identifying associated proteins. This product is ideal for detecting the expression levels of c-Myc fusion proteins by various immunoassays.
Cellular location
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| WB | |
|---|---|
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Lysates: 1. HEK-293T cells transfected with an empty vector (vector control); 2. HEK-293F cells transfected with BCL2 (WT) (26 kDa) expression vector containing a Myc-tag, whole cell lysate |
| ICC/IF | |
|---|---|
|
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Samples: (A) 293T cells; (B) 293T OE cells |
| IP | |
|---|---|
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IP of HEK-293F transfected with BCL2 (WT) (26 kDa) expression vector containing a Myc-tag, whole cell lysate |
Research Use
For research use only, not for use in diagnostic procedures.
