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Anti-O-Linked N-Acetylglucosamine Mouse mAb
Catalog Number: PTM-952
$ 350

Clone Number: 2B4

Host: Mouse Clonality: Monoclonal

Applications： WB ICC/IF

Reactivity: Human, Mouse, Rat

Synonyms: O-GlcNAc

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms O-GlcNAc
UniProt ID

/

Immunogen
MW (kDa)
Specificity
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:1000 All
ICC/IF 1:50 Human
Properties
Purity Protein G and immunogen affinity purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

O-Linked β-N-acetylglucosamine modification (GlcNAcylation) is a glycosylation in which the monosaccharide β-N-acetylglucosamine (GlcNAc) attaches to serine/threonine residues via an O-linked glycosidic bond and is found mostly within the cytoplasm or nucleoplasm. GlcNAcylation regulates several important cellular processes, such as signal transduction, protein expression, degradation, embryonic stem cell pluripotency and trafficking. This post-translational modification is regulated by OGT and O-GlcNAcase (β-N-acetylglucosaminidase) enzymes, whereas OGT catalyzes the attachment and O-GlcNAcase catalyzes the removal of O-GlcNAc to proteins. O-GlcNAc modification has been observed on histones: H2A at T101, H2B at S36 and S112, H3 at S10 and T32, and H4 at S47.

Cellular location

/

Images
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)
Lysate: (-) HeLa, (+) HeLa+PUGNAC (100 μM, 24hr)
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted band size: Multiple kDa
Observed band size: Multiple kDa

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)
Lysate: HeLa, N2a, BRL
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted band size: Multiple kDa
Observed band size: Multiple kDa
ICC/IF

Cell line: (A) HeLa, (B) HeLa+PUGNAC
(100mM, 24hr)
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% TritonX-100
Primary Ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary Ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (Blue)
Description: The green color represents the positive signal observed with PTM-952

Research Use

For research use only, not for use in diagnostic procedures.

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    $ 340
    Recombinant Rabbit Monoclonal
    Anti-O-Linked N-Acetylglucosamine Rabbit mAb
    Catalog #
    PTM-951RM
    Host
    Rabbit
    Clonality
    Recombinant Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Mouse, Rat
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
References (4)
  • TFAM loss induces nuclear actin assembly upon mDia2 malonylation to promote liver cancer metastasis
    Year 2022
    Journal EMBO JOURNAL
    Authors Qichao Huang, et al.
    Applications Unspecified
    Reactivity
  • Branched-chain amino acid catabolism breaks glutamine addiction to sustain hepatocellular carcinoma progression
    Year 2022
    Journal Cell Reports
    Authors Dongdong Yang, et al.
    Applications Unspecified
    Reactivity
  • Appetite Suppression and Interleukin 17 Receptor Signaling Activation of Colonic Mycobiota Dysbiosis Induced by High Temperature and High Humidity Conditions
    Year 2021
    Journal Frontiers in Cellular and Infection Microbiology
    Authors Yinrui Guo, et al.
    Applications Unspecified
    Reactivity
  • Global profiling of O-GlcNAcylated and/or phosphorylated proteins in hepatoblastoma
    Year 2019
    Journal Signal Transduction and Targeted Therapy
    Authors Song Hang, et al.
    Applications Unspecified
    Reactivity
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