Clone Number: 2B4
Host: Mouse Clonality: Monoclonal
Applications： WB ICC/IF
Reactivity: All
Synonyms: O-GlcNAc
Shipping: Ambient temperature
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| Isotype | IgG |
| Conjugate | Unconjugated |
| Synonyms | O-GlcNAc |
| UniProt ID | |
| Immunogen | Synthetic peptides (proprietary to PTM BIO) |
| MW (kDa) | Multiple |
| Specificity | Anti-O-Linked N-Acetylglucosamine Mouse mAb detects proteins with O-Linked N-Acetylglucosamine (O-GlcNAc) modifications on serine or threonine residues. This pan antibody recognizes O-GlcNAc independent of its surrounding sequences. |
| Applications | Dilution | Recommended Species |
|---|---|---|
| WB | 1:500 - 1:1000 | All |
| ICC/IF | 1:50 | Human |
| Purity | Protein G and immunogen affinity purified |
| Constituents | PBS, Glycerol, BSA |
| Storage | Store at -20°C. Avoid freeze/thaw cycles. |
| Stability | Stable for 12 months from date of receipt/reconstitution. |
Background
O-Linked β-N-acetylglucosamine modification (O-GlcNAcylation) is a glycosylation in which the monosaccharide β-N-acetylglucosamine (GlcNAc) is attached to serine/threonine residues via an O-linked glycosidic bond. This modification is found mostly within the cytoplasm or nucleoplasm. O-GlcNAcylation regulates several important cellular processes, including signal transduction, protein expression, degradation, trafficking, and embryonic stem cell pluripotency. This post-translational modification is regulated by O-GlcNAc transferases (OGT), which catalyzes the addition of GlcNAc, and β-N-acetylglucosaminidase (O-GlcNAcase), which mediates its removal. O-GlcNAcylation has been identified on histones, including H2A at T101, H2B at S36 and S112, H3 at S10 and T32, and H4 at S47.
Cellular location
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| WB | |
|---|---|
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Lysates: HeLa, N2a, and BRL cells |
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Lysates: (-) HeLa cells; (+) HeLa cells treated with 100 mM PUGNAC for 24 hours |
| ICC/IF | |
|---|---|
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Samples: (A) HeLa cells; (B) HeLa cells treated with 100 mM PUGNAC for 24 hours |
Research Use
For research use only, not for use in diagnostic procedures.
