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Recombinant Rabbit Monoclonal
Anti-O-Linked N-Acetylglucosamine Rabbit mAb
Catalog Number: PTM-951RM
$ 330

Clone Number: 17H2L2

Host: Rabbit Clonality: Recombinant Monoclonal

Applications： WB IHC-P ICC/IF

Reactivity: Human, Mouse, Rat

Synonyms: O-GlcNAc

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms O-GlcNAc
UniProt ID

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Immunogen
MW (kDa) Multiple
Specificity Anti-O-Linked N-Acetylglucosamine Rabbit mAb detects proteins with O-Linked N-Acetylglucosamine residues. This pan antibody recognizes O-Linked N-Acetylglucosamine independent of its surrounding sequences.
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:1000 All
ICC/IF 1:50 Human
IHC-P 1:200 - 1:1000 Mouse、Rat
Properties
Purity Protein A purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

O-Linked β-N-acetylglucosamine modification (O-GlcNAcylation) is a glycosylation in which the monosaccharide β-N-acetylglucosamine (GlcNAc) is attached to serine/threonine residues via an O-linked glycosidic bond. This modification is found mostly within the cytoplasm or nucleoplasm. O-GlcNAcylation regulates several important cellular processes, including signal transduction, protein expression, degradation, trafficking, and embryonic stem cell pluripotency. This post-translational modification is regulated by O-GlcNAc transferases (OGT), which catalyzes the addition of GlcNAc, and β-N-acetylglucosaminidase (O-GlcNAcase), which mediates its removal. O-GlcNAcylation has been identified on histones, including H2A at T101, H2B at S36 and S112, H3 at S10 and T32, and H4 at S47.

Cellular location

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Images
Dot Blot

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Immunogen peptide quantity: 4 ng, 20 ng, 100 ng
Exposure time: 60 s
The list of peptides is included in the table below.
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Lysate: (-) HeLa, (+) HeLa+PUGNAC (100μM, 24hr)
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted band size: Multiple kDa
Observed band size: Multiple kDa

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Lysate: BRL, Mouse liver, Mouse colon
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted band size: Multiple kDa
Observed band size: Multiple kDa
ICC/IF

Cell line: HepG2
Fixative: 100% Ice-cold methanol
Permeabilization: 0.1% TritonX-100
Primary Ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary Ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (Blue)
Description: The green color represents the positive signal observed with PTM-951RM

Cell line: MCF-7
Fixative: 100% Ice-cold methanol
Permeabilization: 0.1% TritonX-100
Primary Ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary Ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (Blue)
Description: The green color represents the positive signal observed with PTM-951RM
IHC-P

Tissue: Mouse cerebrum
Section type: Formalin-fixed & Paraffinembedded section
Retrieval method: High temperature and high
pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation: 1 hour at
room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer
HRP (Ready to use)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for
PTM-951RM

Tissue: Rat cerebrum
Section type: Formalin-fixed & Paraffinembedded section
Retrieval method: High temperature and high
pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation: 1 hour at
room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer
HRP (Ready to use)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for
PTM-951RM

Research Use

For research use only, not for use in diagnostic procedures.

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  • View More Images（2）
    $ 495
    Recombinant Rabbit Monoclonal
    Anti-O-Linked N-Acetylglucosamine Rabbit mAb (BSA and Azide Free)
    Catalog #
    PTM-955RM
    Host
    Rabbit
    Clonality
    Recombinant Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    All
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P CUT&Tag
References (2)
  • O-GlcNAcylation regulation of RIPK1-dependent apoptosis dictates sensitivity to sunitinib in renal cell carcinoma
    Year 2024
    Journal DRUG RESISTANCE UPDATES
    Authors Xiangbo Zeng, et al.
    Applications Unspecified
    Reactivity
  • The Development of Aspergillus flavus and Biosynthesis of Aflatoxin B1 are Regulated by the Golgi-Localized Mn2+ Transporter Pmr1
    Year 2024
    Journal JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
    Authors Su Qu, et al.
    Applications Unspecified
    Reactivity
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