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Recombinant Rabbit Monoclonal
Anti-O-Linked N-Acetylglucosamine Rabbit mAb
Catalog Number: PTM-951RM
References (7)
$ 330

Clone Number: 17H2L2

Host: Rabbit Clonality: Recombinant Monoclonal

Applications： WB IHC-P ICC/IF

Reactivity: Human, Mouse, Rat

Synonyms: O-GlcNAc

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms O-GlcNAc
UniProt ID

/

Immunogen Synthetic O-GlcNAc-modified peptides
MW (kDa) Multiple
Specificity Anti-O-Linked N-Acetylglucosamine Rabbit mAb detects proteins with O-Linked N-Acetylglucosamine residues. This pan antibody recognizes O-Linked N-Acetylglucosamine independent of its surrounding sequences.
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:1000 All
ICC/IF 1:50 Human
IHC-P 1:200 - 1:1000 Mouse、Rat
Properties
Purity Protein A purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

O-Linked β-N-acetylglucosamine modification (O-GlcNAcylation) is a glycosylation in which the monosaccharide β-N-acetylglucosamine (GlcNAc) is attached to serine/threonine residues via an O-linked glycosidic bond. This modification is found mostly within the cytoplasm or nucleoplasm. O-GlcNAcylation regulates several important cellular processes, including signal transduction, protein expression, degradation, trafficking, and embryonic stem cell pluripotency. This post-translational modification is regulated by O-GlcNAc transferases (OGT), which catalyzes the addition of GlcNAc, and β-N-acetylglucosaminidase (O-GlcNAcase), which mediates its removal. O-GlcNAcylation has been identified on histones, including H2A at T101, H2B at S36 and S112, H3 at S10 and T32, and H4 at S47.

Cellular location

/

Images
Dot Blot

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Immunogen peptide quantity: 4 ng, 20 ng, 100 ng
Exposure time: 60 s
The list of peptides is included in the table below.
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Lysate: (-) HeLa, (+) HeLa+PUGNAC (100μM, 24hr)
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted band size: Multiple kDa
Observed band size: Multiple kDa

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Lysate: BRL, Mouse liver, Mouse colon
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted band size: Multiple kDa
Observed band size: Multiple kDa
ICC/IF

Cell line: HepG2
Fixative: 100% Ice-cold methanol
Permeabilization: 0.1% TritonX-100
Primary Ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary Ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (Blue)
Description: The green color represents the positive signal observed with PTM-951RM

Cell line: MCF-7
Fixative: 100% Ice-cold methanol
Permeabilization: 0.1% TritonX-100
Primary Ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary Ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (Blue)
Description: The green color represents the positive signal observed with PTM-951RM
IHC-P

Tissue: Mouse cerebrum
Section type: Formalin-fixed & Paraffinembedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for PTM-951RM

Tissue: Rat cerebrum
Section type: Formalin-fixed & Paraffinembedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for PTM-951RM

Research Use

For research use only, not for use in diagnostic procedures.

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    $ 495
    Recombinant Rabbit Monoclonal
    Anti-O-Linked N-Acetylglucosamine Rabbit mAb (BSA and Azide Free)
    Catalog #
    PTM-955RM
    Host
    Rabbit
    Clonality
    Recombinant Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    All
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P CUT&Tag
References (7)
  • Glutamine alleviates immunosuppression in polymicrobial sepsis by augmenting bacterial phagocytosis through sustaining the GFAT-DRP1 dependent mitochondrial calcium dynamics
    Year 2025
    Journal CLINICAL SCIENCE
    Authors Yuanfeng Zhu, et al.
    Applications Unspecified
    Reactivity
  • O-GlcNAcylated Hsp47 as a predictive biomarker in colorectal cancer: Kaempferol targets OGT-collagen axis for therapeutic intervention
    Year 2025
    Journal International Journal of Biological Sciences
    Authors Chishun Zhou, et al.
    Applications Unspecified
    Reactivity
  • Bone Morphogenetic Protein 4 Ameliorates Corneal Neovascularization by Inhibiting NLRP3 Inflammasome Activation through the Promotion of CBP-Mediated S100A9 Acetylation
    Year 2025
    Journal FREE RADICAL BIOLOGY AND MEDICINE
    Authors Wenxin Zhang, et al.
    Applications Unspecified
    Reactivity
  • O-GlcNAcylated FTO promotes m6A modification of SOX4 to enhance MDS/AML cell proliferation
    Year 2025
    Journal Cell Communication and Signaling
    Authors Gou Junjie, et al.
    Applications Unspecified
    Reactivity
  • O-GlcNAcylation regulation of RIPK1-dependent apoptosis dictates sensitivity to sunitinib in renal cell carcinoma
    Year 2024
    Journal DRUG RESISTANCE UPDATES
    Authors Xiangbo Zeng, et al.
    Applications Unspecified
    Reactivity
  • The Development of Aspergillus flavus and Biosynthesis of Aflatoxin B1 are Regulated by the Golgi-Localized Mn2+ Transporter Pmr1
    Year 2024
    Journal JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
    Authors Su Qu, et al.
    Applications Unspecified
    Reactivity
  • TMT-labeled quantitative malonylome analysis on the longissimus dorsi muscle of Laiwu pigs reveals the role of ACOT7 in fat deposition
    Year 2024
    Journal Journal of Proteomics
    Authors Wenlei Wang, et al.
    Applications WB
    Reactivity
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