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Recombinant
Anti-Occludin Mouse mAb
Catalog Number: PTM-5252
$ 280

Clone Number: 13187

Host: Mouse Clonality: Recombinant Monoclonal

Applications: WB ICC/IF

Reactivity: Human

Synonyms: OCLN

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG1/kappa
Conjugate Unconjugated
Synonyms OCLN
UniProt ID

Q16625

Immunogen
MW (kDa)
Specificity
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:1000 Human
ICC/IF 1:50 Human
Properties
Purity Protein G purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Occludin is a component of tight junctions. Occludin is thought to be important in the assembly and maintenance of tight junctions. Differential phosphorylation of occludin at various residues may regulate its interaction with other tight junction proteins such as ZO-1. VEGF-induced phosphorylation of occludin regulates tight junction stability and vascular permeability. Expression of occludin as well as claudin1 is required for infection of liver cells by hepatitis C virus (HCV).

Cellular location

Cell membrane

Images
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Mouse IgGH&L(HRP Conjugate)
Lysate: 1: 293, 2: HUVEC, 3: Caco-2
Protein loading quantity: 20 μg
Exposure time: 1, 2: 10 s, 3: 60 seconds
Predicted band size: 59 kDa
Observed band size: 59 kDa

ICC/IF

Cell line: Caco-2
Fixative: 100% Ice-cold ethanol
Permeabilization: 0.1% Triton X-100
Primary Ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary Ab: Goat Anti-Mouse IgG
Nuclear counter stain: DAPI (Blue)
Description: The green color represents the positive signal observed with PTM-5252

Research Use

For research use only, not for use in diagnostic procedures.