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Recombinant Rabbit Monoclonal
Anti-p53 Rabbit mAb
Catalog Number: PTM-6517
$ 290

Clone Number: JRMR-10831-21

Host: Rabbit Clonality: Recombinant Monoclonal

Applications: WB

Reactivity: Human

Synonyms: TP53

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms TP53
UniProt ID

P04637

Immunogen
MW (kDa)
Specificity
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:2000 Human
Properties
Purity Protein A purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Cellular tumor antigen p53 acts as a tumor suppressor in many tumor types. Its function for inducing growth arrest or apoptosis is depended on the physiological circumstances and cell type. p53 is involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process.

Cellular location

Nucleus, Cytoplasm, Mitochondrion, Cytoskeleton

Images
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Lysate: 1: Saos-2 (Negative control), 2: A431, 3: HT-29, 4: HEK-293
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted band size: 44 kDa
Observed band size: 53 kDa

Research Use

For research use only, not for use in diagnostic procedures.

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