中 / EN
Back
QUICK ORDER
Technical Support
| Contact Us
+1 312-802-6843
Technical Support
Contact Us
+1 312-802-6843
中 / EN
QUICK ORDER
Proteomics Bioinformation Research Antibody Products IVD Products Custom Antibody Development
Recover password
Professional Email
Verification Code
Send Code
Password
Recombinant Rabbit Monoclonal
Anti-p60 CAF1 / MPP7 Rabbit mAb
Catalog Number: PTM-6338
$ 285

Clone Number: JRMR-10175-32

Host: Rabbit Clonality: Recombinant Monoclonal

Applications： WB IHC-P ICC/IF IP

Reactivity: Human

Synonyms: CHAF1B, CAF1A, CAF1P60, MPHOSPH7

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms CHAF1B, CAF1A, CAF1P60, MPHOSPH7
UniProt ID

Q13112

Immunogen Recombinant human p60 CAF1 / MPP7 fragment
MW (kDa) 70
Specificity Anti-p60 CAF1 / MPP7 Rabbit mAb recognizes endogeneous level of p60 CAF1 / MPP7 protein.
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:1000 Human
IHC-P 1:50 - 1:100 Human
ICC/IF 1:50 Human
IP 1:25 - 1:50 Human
Properties
Purity Protein A purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Chromatin assembly factor 1 p60 (p60 CAF1) is a subunit of Chromatin assembly factor I (CAF-I) which is required for the assembly of histone octamers onto newly-replicated DNA. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer.

Cellular location

Nucleus, cytoplasm

Images
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Lysate: 1: Jurkat, 2: K562
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted band size: 61 kDa
Observed band size: 70 kDa
IHC-P

Tissue: Human tonsil
Section type: Formalin-fixed & Paraffin_x0002_embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:100
Primary Ab incubation: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (Blue)
Description: The brown color represents the positive signal observed with PTM-6338
ICC/IF

Cell line: 293T
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% TritonX-100
Primary Ab dilution: 1:50
Primary Ab incubation: 4°C overnight
Secondary Ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (Blue)
Counter stain: Tubulin (Red)
Description: The green color represents the positive signal observed with PTM-6338

Cell line: TF-1
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% TritonX-100
Primary Ab dilution: 1:50
Primary Ab incubation: 4°C overnight
Secondary Ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (Blue)
Counter stain: Tubulin (Red)
Description: The green color represents the positive signal observed with PTM-6338
IP

IP of Jurkat cells extracts
IP Ab incubation: PTM-6338, 4°C overnight, 1:50 dilution
WB Primary Ab incubation: PTM6338, room temperature 2h, 1:1000 dilution
Secondary Ab: Anti-Rabbit IgG for IP (HRP)
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
1:10% Input
2: IP with PTM-6338
3: IP with Rabbit monoclonal IgG Isotype
Observed MW: 70 kDa
Exposure time: 60 s

Research Use

For research use only, not for use in diagnostic procedures.

Service
Products
Support
About Us
© 2023 PTM Biolabs Inc.