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Recombinant Rabbit Monoclonal
Anti-Pan-Actin Rabbit mAb
Catalog Number: PTM-5447
$ 280

Clone Number: JRMR-10150-22

Host: Rabbit Clonality: Recombinant Monoclonal

Applications： WB IHC-P ICC/IF FC

Reactivity: Human, Mouse, Rat

Synonyms: Alpha-actin-1, Alpha-actin-2, Alpha-actin-3, Alpha-cardiac actin, Beta-actin, Gamma-actin

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms Alpha-actin-1, Alpha-actin-2, Alpha-actin-3, Alpha-cardiac actin, Beta-actin, Gamma-actin
UniProt ID

P60709, P68133, P63261, P68032, P62736, P63267

Immunogen
MW (kDa)
Specificity
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:1000 Human, Mouse, Rat
IHC-P 1:200 - 1:1000 Human, Mouse, Rat
ICC/IF 1:50 Human, Mouse
FC 1:50 - 1:100 Human
Properties
Purity Protein A purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.

Cellular location

Cytoskeleton

Images
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Lysate: HeLa, Jurkat, HEK293, NIH/3T3,
C2C12, Mouse brain, Rat brain
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted band size: 42 kDa
Observed band size: 42 kDa
IHC-P

Tissue: Human prostate hyperplasia
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (Blue)
Description: The brown color represents the positive signal observed with PTM-5447

Tissue: Human skeletal muscle
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (Blue)
Description: The brown color represents the positive signal observed with PTM-5447

Tissue: Mouse cardiac muscle
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (Blue)
Description: The brown color represents the positive signal observed with PTM-5447

Tissue: Rat stomach
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (Blue)
Description: The brown color represents the positive signal observed with PTM-5447
ICC/IF

Cell line: HeLa
Fixative: 100% Ice-cold methanol
Permeabilization: 0.1% TritonX-100
Primary Ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary Ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (Blue)
Description: The green color represents the positive signal observed with PTM-5447

Cell line: A431
Fixative: 100% Ice-cold methanol
Permeabilization: 0.1% TritonX-100
Primary Ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary Ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (Blue)
Description: The green color represents the positive signal observed with PTM-5447

Cell line: NIH/3T3
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% TritonX-100
Primary Ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary Ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (Blue)
Description: The green color represents the positive signal observed with PTM-5447
FC

Cell line: Jurkat
Fixative: 4% Paraformaldehyde
Permeabilization: 90% Methanol
Primary Ab dilution: 1:100
Secondary Ab: Goat anti Rabbit IgG
Unlabeled control: Cells without incubation with primary and secondary antibodies (Black line).
Isotype control: Rabbit monoclonal IgG (Blue line).
Description: Line red is the positive signal for PTM-5447

Research Use

For research use only, not for use in diagnostic procedures.

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References (1)
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