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Recombinant
Anti-PARP1 Mouse mAb
Catalog Number: PTM-5171
$ 280

Clone Number: 11152

Host: Mouse Clonality: Monoclonal

Applications: WB

Reactivity: Human, Mouse

Synonyms: ARTD1, ADPRT 1, ADPRT, PPOL

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG2a/kappa
Conjugate Unconjugated
Synonyms ARTD1, ADPRT 1, ADPRT, PPOL
UniProt ID

P09874

Immunogen
MW (kDa)
Specificity
Product Usage Information
Applications Dilution Recommended Species
WB 1:1000 Human,Mouse
Properties
Purity Protein G purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Poly-ADP-ribosyltransferase that mediates poly-ADP-ribosylation of proteins and plays a key role in DNA repair. Mainly mediates glutamate and aspartate ADP-ribosylation of target proteins: the ADP-D-ribosyl group of NAD+ is transferred to the acceptor carboxyl group of glutamate and aspartate residues and further ADP-ribosyl groups are transferred to the 2'-position of the terminal adenosine moiety, building up a polymer with an average chain length of 20-30 units. Mediates the poly(ADP-ribosyl)ation of a number of proteins, including itself, APLF and CHFR. Also mediates serine ADP-ribosylation of target proteins following interaction with HPF1; HPF1 conferring serine specificity.

Cellular location

Chromosome, Nucleus

Images
WB

Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:1000
Primary ab incubation condition: 2 hours at room temperature
Lysate: HeLa, HepG2, Neuro-2a, EL4.IL-2
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 113 kDa
Observed MW: 85/113 kDa

Research Use

For research use only, not for use in diagnostic procedures.