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Recombinant Rabbit Monoclonal
Anti-PBRM1/BAF180 Rabbit mAb
Catalog Number: PTM-7111
$ 280

Clone Number: JRMR-11094-2

Host: Rabbit Clonality: Recombinant Monoclonal

Applications: WB

Reactivity: Human, Rat

Synonyms: Protein polybromo-1, hPB1, Polybromo-1D, PB1

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms Protein polybromo-1, hPB1, Polybromo-1D, PB1
UniProt ID

Q86U86

Immunogen
MW (kDa)
Specificity
Product Usage Information
Applications Dilution Recommended Species
WB 1:2000 - 1:5000 Human, Rat
Properties
Purity Protein A purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

PBRM1/BAF180 is a unique member of the SWI/SNF complex PBAF, which binds to kinetochores in mitotic chromatin. PBAF is involved in nuclear receptor-mediated transcription and retinoic acid driven gene activation. PBRM1/BAF180 has been shown to be a potent tumor suppressor, as it is the second-most mutated gene in renal carcinomas. Mutations of PBRM1/BAF180 have also been shown to be involved in breast cancer, and low expression relates to poorer prognosis. PBRM1/BAF180 is phosphorylated at Ser948 by ATM during DNA damage, which is important for transcriptional silencing and repair around double-stranded breaks.

Cellular location

Nucleus

Images
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:5000
Primary Ab incubation: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Lysate: 1: MCF-7, 2: MOLT-4, 3: PC-12
Protein loading amount: 20 μg
Exposure time: 60 seconds
Predicted band size: 193 kDa
Observed band size: 193 kDa

Research Use

For research use only, not for use in diagnostic procedures.