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Recombinant Rabbit Monoclonal
Anti-PEG10 Rabbit mAb
Catalog Number: PTM-7066
$ 280

Clone Number: JRMR-10844-18

Host: Rabbit Clonality: Recombinant Monoclonal

Applications: WB

Reactivity: Human, Mouse, Rat

Synonyms: EDR

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms EDR
UniProt ID

Q86TG7

Immunogen
MW (kDa) 55/100
Specificity
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:1000 Human, Mouse, Rat
Properties
Purity Protein A purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Paternally expressed gene 10(PEG10 ) is an imprinted gene which is derived from a Ty3/Gypsy long terminal repeat (LTR) retrotransposon family encoding Gag- and Pol-like domains. Deletion of PEG10 in mice leads to embryonic lethality due to defects in placental formation. PEG10 is aberrantly expressed in several cancer types, including hepatocellular carcinoma, and contributes to tumorigenesis by affecting cell proliferation, apoptosis, and metastasis. The PEG10 gene has two overlapping open reading frames that are regulated by the programmed process of -1 frameshifting. The first encoded protein ORF1 (or RF1) has a Gag domain with coiled-coil domain and zinc finger domains, while ORF1-2 (or RF1/RF2) is produced by -1 frameshifting and creates a fusion of the ORF1 Gag domain with a carboxyl-terminal Pol-like protease domain. PEG10 has retained the ability to form virus-like particles (VLPs) that are secreted as small extracellular vesicles delivered to distant sites.

Cellular location

Cytoplasm, Cell membrane, Nucleus

Images
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgGH&L (HRP Conjugate)
Lysate: 1: HepG2, 2: HeLa, 3: Mouse placenta, 4: Rat placenta
Protein loading quantity: 20 μg
Exposure time: 60 seconds
Predicted band size: 55/100 kDa
Observed band size: 55/100 kDa

Research Use

For research use only, not for use in diagnostic procedures.