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Recombinant Rabbit Monoclonal
Anti-PHF8 Rabbit mAb
Catalog Number: PTM-6496
$ 290

Clone Number: JMMR-2573

Host: Rabbit Clonality: Recombinant Monoclonal

Applications: WB

Reactivity: Human, Mouse, Rat

Synonyms: KIAA1111, ZNF422

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms KIAA1111, ZNF422
UniProt ID

Q9UPP1

Immunogen
MW (kDa)
Specificity
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:1000 Human, Mouse, Rat
Properties
Purity Protein A purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

PHF8 is a histone lysine demethylase that functions as a transcriptional activator by specifically demethylating a number of repressive histone methylation marks: mono- and Dimethyl-histone H3 Lys9 (H3K9me1 and H3K9me2), Dimethyl-histone H3 Lys27 (H3K27me2) and Monomethyl-histone H4 Lys20 (H4K20me1). PHF8 contains an N-terminal zinc finger-like PHD domain that binds Trimethylated histone H3 Lys4 (H3K4Me3) and a C-terminal jumonji domain that is responsible for the demethylase activity. PHF8 is highly expressed in prostate cancer, laryngeal squamous cell carcinoma, and human non-small-cell lung cancer (NSCLC). Its expression is predictive of poor survival. Overexpression of PHF8 increases cell proliferation and cell motility, while silencing of PHF8 reduces cell proliferation, migration, and invasion.

Cellular location

Nucleus

Images
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Lysate: 1: HeLa, 2: HEK-293, 3: NIH/3T3, 4: PC-12
Protein loading quantity: 20 μg
Exposure time: 60 seconds
Predicted band size: 118 kDa
Observed band size: 140 kDa

Research Use

For research use only, not for use in diagnostic procedures.