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Recombinant
Anti-Phospho-HDAC1 (Ser406) Mouse mAb
Catalog Number: PTM-755
$ 280

Clone Number: 12851

Host: Mouse Clonality: Recombinant Monoclonal

Applications: WB

Reactivity: Human

Synonyms: HD1, RPD3L1

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG2b
Conjugate Unconjugated
Synonyms HD1, RPD3L1
UniProt ID

Q13547

Immunogen
MW (kDa)
Specificity
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:1000 Human
Properties
Purity Protein G purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Aurora kinases phosphorylate HDAC1 at Ser406 during prophase, immediately after cells begin mitosis, resulting in reduced deacetylase activity of HDAC1. This modification plays an essential role in regulating cell cycle progression, as well as controlling the expression of genes involved in central nervous system development.

Cellular location

Nucleus

Images
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation: 2 hours at room temperature
Lysate: HeLa untreated (-) or treated (+) with 100ng/ml Nocodazole ,18h.
Protein loading quantity: 20 μg
Exposure time: 3 s
Predicted band size: 55 kDa
Observed band size: 60 kDa

Research Use

For research use only, not for use in diagnostic procedures.