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Anti-Phospho-Histone H2A.X (Ser139) Mouse mAb
Catalog Number: PTM-736
$ 340

Clone Number: /

Host: Mouse Clonality: Monoclonal

Applications: WB IP

Reactivity: Human, Mouse, Rat

Synonyms: H2A.XS139ph

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms H2A.XS139ph
UniProt ID

P16104

Immunogen
MW (kDa) 15
Specificity
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:2000 Human, Mouse, Rat
IP 1:25 Human
Properties
Purity Protein G and immunogen affinity purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Histones are subjected to a variety of enzyme catalyzed modifications, including acetylation, methylation, ubiquitylation and newly discovered succinylation, crotonylation and 2-hydroxyisobutyrylation, etc. These modifications have a direct effect on the accessibility of chromatin to transcription factors and, therefore, on gene expression. Histone H2A.x is a ubiquitously expressed variant of histone H2A and phosphorylation of histone H2A.x at Ser139 (termed γ-H2AX) is located in a unique C-terminal region and regulated by various kinases, including the ataxia telangiectasia-mutated (ATM) protein, the AT and Rad3-related protein, and DNA-dependent protein kinase, all of which were reported to be involved in the responses of mammalian cells to DNA double-strand breaks.

Cellular location

Nucleus

Images
Dot Blot

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Mouse IgG H&L pAb(HRP Conjugate)
Immunogen peptide quantity: 1 ng, 4 ng, 16 ng
Exposure time: 60 s
The list of peptides is included in the table below.

WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)
Lysate: (-) HeLa, (+) HeLa+ Nocodazole(100ng/ml, 18hr) + Calyculin A (100nM, 1hr)
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted band size: 15 kDa
Observed band size: 15 kDa

IP

IP of HeLa+ Nocodazole (100ng/ml, 18hr) + Calyculin A (100nM, 1hr) cells extracts
IP Ab incubation condition: PTM-736, 4°C overnight, 1:10, 1:25 dilution
WB Primary Ab incubation condition: PTM716 (Anti-H2A.xS139ph Rabbit pAb), room temperature 2h, 1:1000 dilution
Secondary Ab: Anti-Rabbit IgG for IP (HRP Conjugate)
Blocking buffer and concentration: 5% NFDM/TBST
Diluting buffer and concentration: 5% NFDM/TBST
Lane 1: 5% Input
Lane 2: IP with PTM-736 (1:10)
Lane 3: IP with PTM-736 (1:25)
Observed band size: 15 kDa
Exposure time: 60 s

IP

IP of HeLa+ Nocodazole (100ng/ml, 18hr) + Calyculin A (100nM, 1hr) cells extracts
IP Ab incubation condition: PTM-736, 4°C overnight, 1:10, 1:25 dilution
WB Primary Ab incubation condition: PTM736, room temperature 2h, 1:1000 dilution
Secondary Ab: Anti-Mouse IgG for IP (HRP Conjugate)
Blocking buffer and concentration: 5% NFDM/TBST
Diluting buffer and concentration: 5% NFDM/TBST
Lane 1: 5% Input
Lane 2: IP with PTM-736 (1:10)
Lane 3: IP with PTM-736 (1:25)
Observed band size: 15 kDa
Exposure time: 60 s

Research Use

For research use only, not for use in diagnostic procedures.

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