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Recombinant Rabbit Monoclonal
Anti-Phospho-Histone H3 (Ser10) Rabbit mAb
Catalog Number: PTM-712RM
$ 330

Clone Number: PD-O0701

Host: Rabbit Clonality: Polyclonal

Applications： WB IP ChIP

Reactivity: Human, Mouse

Synonyms: H3S10ph

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms H3S10ph
UniProt ID

P68431

Immunogen
MW (kDa) 15
Specificity Anti-Phospho-Histone H3 (Ser10) Rabbit mAb detects histone H3 only when it is Phospho Histoneated at Ser10.
Product Usage Information
Applications Dilution Recommended Species
WB 1:1000 - 1:5000 Human, Mouse
ChIP 6ug抗体/100ug染色质 Human
IP 1:50 - 1:100 Human
Properties
Purity Protein A purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. Crotonylation of lysine is a newly identified reversible modification controlling chromosome structure and gene transcription. The reversible lysine crotonylation has been well demonstrated in eukaryotic histones from worm to human. The unique structure and genomic localization of histone lysine crotonylation suggest that it is mechanistically and functionally different from histone lysine acetylation. Specifically, in both human somatic and mouse male germ cell genomes, histone crotonylation marks either active promoters or potential enhancers. Crotonylation of histone H4 at Lys5 may play a vital role in the epigenetic modulation, including chromatin remodeling and DNA transcriptional regulation.

Cellular location

Nucleus

Images
Dot Blot

Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:5000 Primary Ab incubation: 2 hours at room temperature Secondary ab: Goat Anti-rabbit IgG H&L (HRP) Peptide amount: 1-2: 1 ng, 4 ng, 16 ng, 64ng Exposure time: 30 s The list of peptides is included in the table below: No. Peptide No. Peptide Lane 1 H3S10ph Lane 2 H3S10un
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:5000
Primary Ab incubation: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: : (-): HeLa, (+):HeLa + Nocodazole (100ng/mL, 18h)
Protein loading amount: 20 μg
Exposure time: 30 s
Predicted MW: 17 kDa
Observed MW: 17 kDa

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: (-): NIH-3T3, (+): NIH-3T3+Serum(0%, overnight) add Nocodazole(100ng/mL, 18h) add Calyculin A (100nM, 1h)
Protein loading amount: 20 μg
Exposure time: 30 s
Predicted MW: 17 kDa
Observed MW: 17 kDa
ChIP

Cell type: HeLa+Nocodazole(100ng/mL, 18h) Cross-linking conditions: no cross-linking Amount of chromatin per IP: 100 μg Amount of Ab per IP: 6 ug Beads type and amount per IP: 50 μl of Protein A MagBeads Comment: The ChIP was performed with 6 μg of normal Rabbit IgG as a negative control. Real time quantitative PCR was performed on immunoprecipitated DNA using primers specific for the human LDHA-promoter and TuBBP10. Data are presented as enrichment of each sample relative to the total amount of input chromatin at each amplicon.
IP

IP of HeLa+Nocodazole(100ng/mL, 18h) cells extracts IP Ab incubation: PTM-712RM, 4°C overnight, 1:100 dilution WB Primary Ab incubation: PTM-712RM, room temperature 2h, 1: 2000 dilution Secondary Ab: Anti-Rabbit IgG for IP (HRP) Blocking buffer: 5% NFDM/TBST Dilution buffer: 5% NFDM/TBST 1: 10% Input 2: IP with PTM-712RM 3: IP with Rabbit monoclonal IgG Isotype Observed MW: 17 kDa Exposure time: 10 s

Research Use

For research use only, not for use in diagnostic procedures.

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