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Anti-Phospho-Histone H3 (Thr3) Rabbit pAb
Catalog Number: PTM-710
$ 330

Clone Number: /

Host: Rabbit Clonality: Polyclonal

Applications: WB IP

Reactivity: Human, Mouse, Rat

Synonyms: H3T3ph

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms H3T3ph
UniProt ID

P68431

Immunogen
MW (kDa)
Specificity
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:2000 Human, Mouse, Rat
IP 1:25 Human
Properties
Purity Protein A and immunogen affinity purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Histone post-translational modifications (PTMs) are key mechanisms of epigenetics that modulate chromatin structures, termed as “histone code”. The PTMs on histone including acetylation, methylation, phosphorylation and novel acylation directly affect the accessibility of chromatin to transcription factors and other epigenetic regulators, altering genome stability, gene transcription, etc. Histone phosphorylation occurs on serine, threonine and tyrosine residues on the amino-terminal of core histones. This histone mark plays roles in DNA repair, transcription and chromatin remodeling. The best-known histone phosphorylation site is histone variant H2A.x Ser139ph, which were reported to be involved in the response to DNA damage. Histone phosphorylation is mainly involved in processes during both mitosis and meiosis. Many kinases and phosphatases regulate histone phosphorylation levels.

Cellular location

Nucleus

Images
Dot Blot

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Immunogen peptide quantity: 1 ng, 4 ng, 16 ng
Exposure time: 60 s
The list of peptides is included in the table below.

WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Lysate: (-) HeLa, (+) HeLa+FBS (20%, 1hr)+Calyculin A (100nM, 15min)
Protein loading quantity: 20 μg
Exposure time: 30 s
Predicted band size: 17 kDa
Observed band size: 17 kDa

IP

IP of HeLa+ Nocodazole (100ng/ml, 18hr) +Calyculin A (100nM, 1hr) cells extracts
IP Ab incubation condition: PTM-710, 4°C overnight, 1:25, 1:100 dilution
WB Primary Ab incubation condition: PTM-710, room temperature 2h, 1:1000 dilution
Secondary Ab: Anti-Rabbit IgG for IP (HRP Conjugate)
Blocking buffer and concentration: 5% NFDM/TBST
Diluting buffer and concentration: 5% NFDM/TBST
Lane 1: 5% Input
Lane 2: IP with PTM-710 (1:25)
Lane 3: IP with PTM-710 (1:100)
Observed band size: 17 kDa
Exposure time: 60 s

Research Use

For research use only, not for use in diagnostic procedures.

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    Anti-Phospho-Histone H3 (Thr3) Mouse mAb
    Catalog #
    PTM-730
    Host
    Mouse
    Clonality
    Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Mouse, Rat
    Applications:
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P