中 / EN
Back
QUICK ORDER
Technical Support
| Contact Us
+1 312-802-6843
Technical Support
Contact Us
+1 312-802-6843
中 / EN
QUICK ORDER
Proteomics Bioinformation Research Antibody Products IVD Products Custom Antibody Development
Recover password
Professional Email
Verification Code
Send Code
Password
Recombinant Rabbit Monoclonal
Anti-Phospho-S6 Ribosomal Protein (Ser240/244) Rabbit mAb
Catalog Number: PTM-5299
$ 300

Clone Number: JRMR-259

Host: Rabbit Clonality: Recombinant Monoclonal

Applications： WB IHC-P ICC/IF IP

Reactivity: Human, Mouse, Rat

Synonyms: RPS6, RS6, S6

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms RPS6, RS6, S6
UniProt ID

P62753

Immunogen phosphorylated human S6 Ribosomal Protein (Ser240/244) peptide
MW (kDa) 32
Specificity This antibody detects S6 Ribosomal Protein only when it is phosphorylated at Ser240/244。
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:1000 Human
IHC-P 1:200 - 1:1000 Human, Mouse, Rat
ICC/IF 1:50 Human
IP 1:25 - 1:50 Human
Properties
Purity Protein A purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Component of the 40S small ribosomal subunit. Plays an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.

Cellular location

Cytosol, Endoplasmic reticulum, Nucleus

Images
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Lysate: (-) MCF-7+Serum starvation overnight, (+) MCF-7+Serum starvation overnight, then grown in 20% FBS media for 30 minute
Protein loading quantity: 20 μg
Exposure time: 10 s
Predicted band size: 29 kDa
Observed band size: 32 kDa
IHC-P

Tissue: Human breast carcinoma
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (Blue)
Description: The brown color represents the positive signal observed with PTM-5299

Tissue: Human colon
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (Blue)
Description: The brown color represents the positive signal observed with PTM-5299

Tissue: Mouse spleen
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (Blue)
Description: The brown color represents the positive signal observed with PTM-5299

Tissue: Rat kidney
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (Blue)
Description: The brown color represents the positive signal observed with PTM-5299

Tissue: Rat spleen
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (Blue)
Description: The brown color represents the positive signal observed with PTM-5299
ICC/IF

Cell line: HeLa
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% TritonX-100
Primary Ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary Ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (Blue)
Description: The green color represents the positive signal observed with PTM-5299
IP

IP of MCF-7+Serum starvation overnight,
then grown in 20% FBS media for 30 minutes cells extracts
IP Ab incubation: PTM-5299, 4°C overnight, 1:50 dilution
WB Primary Ab incubation: PTM5299, room temperature 2h, 1:1000 dilution
Secondary Ab: Anti-Rabbit IgG for IP (HRP)
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
1: 5% Input
2: IP with PTM-5299
3: IP with Rabbit monoclonal IgG Isotype
Observed MW: 32 kDa
Exposure time: 10 s

Research Use

For research use only, not for use in diagnostic procedures.

Recommended Products
Compatible Secondaries Isotype Control Writers/Readers/Erasers Related Products
  • View More Images（5）
    $ 310
    Recombinant Rabbit Monoclonal
    Anti-Phospho-S6 Ribosomal Protein (Ser235/236) Rabbit mAb
    Catalog #
    PTM-5321
    Host
    Rabbit
    Clonality
    Recombinant Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Mouse
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P CUT&Tag
  • View More Images（2）
    $ 280
    Recombinant
    Anti-S6 Ribosomal Protein Mouse mAb
    Catalog #
    PTM-5592
    Host
    Mouse
    Clonality
    Recombinant Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Mouse
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P CUT&Tag
  • View More Images（2）
    $ 300
    Recombinant
    Anti-Phospho-S6 Ribosomal Protein (Ser235/236) Mouse mAb
    Catalog #
    PTM-5788
    Host
    Mouse
    Clonality
    Recombinant Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    Human
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P CUT&Tag
  • View More Images（1）
    $ 280
    Recombinant
    Anti-S6 Ribosomal Protien Mouse mAb
    Catalog #
    PTM-5862
    Host
    Mouse
    Clonality
    Recombinant Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Mouse, Rat
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P CUT&Tag
  • View More Images（3）
    $ 310
    Recombinant Rabbit Monoclonal
    Anti-Phospho-S6 Ribosomal Protein (Ser235/236) Rabbit mAb
    Catalog #
    PTM-6885
    Host
    Rabbit
    Clonality
    Recombinant Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Mouse
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P CUT&Tag
Service
Products
Support
About Us
© 2025 PTM BIO LLC, or its affiliates