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Recombinant Rabbit Monoclonal
Anti-Propionyl-Histone H2A (Lys125) Rabbit mAb
Catalog Number: PTM-217RM
$ 420

Clone Number: PA-076-08

Host: Rabbit Clonality: Recombinant Monoclonal

Applications： WB ChIP

Reactivity: Human

Synonyms: H2AK125pr

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms H2AK125pr
UniProt ID

P04908

Immunogen Propionylated human histone H2A (Lys125) peptide
MW (kDa) 14
Specificity Anti-Propionyl-Histone H2A (Lys125) Rabbit mAb detects histone H2A only when it is propionylated at Lys125, but not the crotonylated and unmodified H2A peptide at Lys125.
Product Usage Information
Applications Dilution Recommended Species
WB 1:2000 - 1:10000 Human
ChIP 6 μg per 5x106 cells Human
Properties
Purity Protein A purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. Lysine propionylation (Kprop) is structurally similar to lysine acetylation, is a newly identified reversible modification controlling protein activity. Lysine propionylation is abundant in both prokaryotes and eukaryotes and has been found in wide ranges of proteins including histones and non-histone substrates, such as p53. Similar to acetylation of histone H3 at Lys12, propionylation of histone H2A at Lys125 may play a vital role in the epigenetic modulation, including chromatin remodeling and transcriptional regulation.

Cellular location

Nucleus

Images
Dot Blot

Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:2000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-rabbit IgG H&L (HRP)
Peptide amount: 1 ng, 4 ng, 16 ng, 64ng
Exposure time: 30 s
The list of peptides is included in the table below:
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: (-):MCF-7, (+):MCF-7+Sodium Butyrate(50mM, 24hr)+Trichostatin A(500ng-ml, 4hr)
Protein loading amount: 20 μg
Exposure time: 30 s
Predicted MW: 14 kDa
Observed MW: 14 kDa

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: (-): HeLa, (+): HeLa+Sodium Butyrate(30mM, 4hr)
Protein loading amount: 20 μg
Exposure time: 30 s
Predicted MW: 14 kDa
Observed MW: 14 kDa
ChIP

Cell type: HeLa treated with Sodium butyrate (5mM, 24hr)
Cross-linking conditions: No cross-linking
Amount of chromatin per IP: 5×106 cells
Amount of Ab per IP: 6 μg
Beads type and amount per IP: 50 μL of Protein A MagBeads
Description:
The ChIP was performed with 6 μg of normal Rabbit IgG as a negative control. Real time quantitative PCR was performed on immunoprecipitated DNA using primers
specific for the human GAPDH CDS region, RPL30, LDHA-promoter, LDHA CDS region, FOXO3a-promoter, FOXO3a-downstream, RAB20 and TuBBP10. Data are presented as enrichment of each sample relative to total amount of input chromatin at each amplicon.

Research Use

For research use only, not for use in diagnostic procedures.

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