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Anti-Propionyl-Histone H3 (Lys14) Rabbit pAb
Catalog Number: PTM-211
$ 435

Clone Number: /

Host: Rabbit Clonality: Polyclonal

Applications： WB

Reactivity: Human, Mouse, Rat

Synonyms: H3K14pr

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms H3K14pr
UniProt ID

P68431

Immunogen Propionylated human histone H3 (Lys14) peptide
MW (kDa) 15
Specificity
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:2000 Human, Mouse, Rat
Properties
Purity Protein A purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. Lysine propionylation (Kprop) is structurally similar to lysine acetylation, is a newly identified reversible modification controlling protein activity. Lysine propionylation is abundant in both prokaryotes and eukaryotes and has been found in wide ranges of proteins, including histones and non-histone substrates, such as p53. Similar to acetylation of histone H3 at Lys12, propionylation of histone H3 at Lys14 may play a vital role in the epigenetic modulation, including chromatin remodeling and transcriptional regulation.

Cellular location

Nucleus

Images
Dot Blot

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Immunogen peptide quantity: 1 ng, 4 ng, 16ng, 64 ng
Exposure time: 60 s
The list of peptides is included in the table below.
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb(HRP Conjugate)
Lysate: (-) HeLa, (+) HeLa+ sodiumbutyrate(30 mM, 4 hours)
Protein loading quantity: 20 μg
Exposure time: 30 s
Predicted band size: 15 kDa
Observed band size: 15 kDa

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb(HRP Conjugate)
Lysate: (-) HeLa, (+) HeLa+ sodiumbutyrate(30 mM, 4 hours)
Protein loading quantity: 20 μg
Exposure time: 30 s
Predicted band size: 15 kDa
Observed band size: 15 kDa

Research Use

For research use only, not for use in diagnostic procedures.

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References (1)
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