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Anti-Propionyl-Histone H3 (Lys18) Mouse mAb
Catalog Number: PTM-214
$ 420

Clone Number: /

Host: Mouse Clonality: Monoclonal

Applications： WB

Reactivity: Human, Mouse, Rat

Synonyms: H3K18pr

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms H3K18pr
UniProt ID

P68431

Immunogen Propionylated human histone H3 (Lys18) peptide
MW (kDa) 15
Specificity Anti-Propionyl-Histone H3 (Lys18) Mouse mAb detects histone H3 only when it is propionylated at Lys18.
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:2000 Human, Mouse, Rat
Properties
Purity Protein G and immunogen affinity purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation,phosphorylation, ubiquitylation, etc. Lysine propionylation (Kprop) is structurally similar to lysine acetylation, is a newly identified reversible modification controlling protein activity. Lysine propionylation is abundant in both prokaryotes and eukaryotes and has been found in wide ranges of proteins including histones and non-histone substrates, such as p53. Similar to acetylation of histone H3 at Lys12, propionylation of histone H3 at Lys18 may play a vital role in the epigenetic modulation, including chromatin remodeling and transcriptional regulation.

Cellular location

Nucleus

Images
Dot Blot

Peptide amount: 1ng, 4 ng, 16 ng
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation: 2 hours at room temperature
Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)
Exposure time: 60 seconds
The list of peptides used in the experiment is provided below.
Lane 1: H3K18 propionyl. Lane 2: H3K14 acetyl.
Lane 3: H3K14 propionyl. Lane 4: H3K18 acetyl.
Lane 5: H3K18 butyryl. Lane 6: H3K18 crotonyl.
Lane 7: H3K23 acetyl. Lane 8: H3K23 propionyl.
Lane 9: H3K23 butyryl. Lane 10: H3K23 crotonyl.
Lane 11: H3K18 unmodified.
WB

Lysates: (-) HeLa cells; (+) HeLa cells treated with 10 mM sodium propionate for 4 hours
Protein loading amount: 20 μg
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation: 2 hours at room temperature
Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)
Exposure time: 60 seconds
Predicted band size: 15 kDa
Observed band size: 15 kDa

Research Use

For research use only, not for use in diagnostic procedures.

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