中 / EN
Back
QUICK ORDER
Technical Support
| Contact Us
+1 312-802-6843
Technical Support
Contact Us
+1 312-802-6843
中 / EN
QUICK ORDER
Proteomics Bioinformation Research Antibody Products IVD Products Custom Antibody Development
Recover password
Professional Email
Verification Code
Send Code
Password
Anti-Propionyl-Histone H3 (Lys23) Rabbit pAb
Catalog Number: PTM-205
$ 435

Clone Number: /

Host: Rabbit Clonality: Polyclonal

Applications： WB IHC-P

Reactivity: Human, Mouse, Rat

Synonyms: H3K23pr

Product Size
100μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms H3K23pr
UniProt ID

P68431

Immunogen Propionylated human histone H3 (Lys23) peptide
MW (kDa) 15
Specificity Anti-Propionyl-Histone H3 (Lys23) Rabbit pAb detects histone H3 only when it is propionylated at Lys23.
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:2000 Human, Mouse, Rat
IHC-P 1:200 - 1:1000 Human, Rat
Properties
Purity Protein A and immunogen affinity purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Histones are subject to a variety of enzyme-catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, and others. Among these modifications, lysine propionylation (Kpr) has emerged as a newly identified reversible modification that plays a crucial role in controlling protein activity. Structurally similar to lysine acetylation, lysine propionylation is prevalent in both prokaryotes and eukaryotes and has been identified in a wide range of proteins, including histones and non-histone substrates like p53. Recent research has unveiled the involvement of stoichiometric complexes comprising lysine acetyltransferase 6A (KAT6A), KAT6B, and bromodomain- and PHD finger-containing protein 1 (BRPF1) in the catalysis of histone H3 Lys23 propionylation (H3K23pr). Importantly, deficiencies in histone propionylation have been associated with developmental disorders and cancer.

Cellular location

Nucleus

Images
Dot Blot

Peptide amount: 1ng, 4 ng, 16 ng
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 60 seconds
The list of peptides used in the experiment is provided below.
Lane 1: H3K23 propionyl. Lane 2: H3K18 acetyl.
Lane 3: H3K23 acetyl. Lane 4: H3K23 butyryl.
Lane 5: H3K23 crotonyl. Lane 6: H3K27 acetyl.
Lane 7: H3K27 butyryl. Lane 8: H3K23 unmodified.
WB

Lysates: (-): HeLa cells; (+): HeLa cells treated with 10 mM sodium propionate for 4 hours
Protein loading amount: 20 μg
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Exposure time: 60 seconds
Predicted band size: 15 kDa
Observed band size: 17 kDa
IHC-P

Tissue: Human spleen
Section type: Formalin fixed & paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:200
Primary Ab incubation: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (blue)
Description: The brown color represents the positive signal observed with PTM-205.

Tissue: Rat stomach
Section type: Formalin fixed & paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (blue)
Description: The brown color represents the positive signal observed with PTM-205.

Research Use

For research use only, not for use in diagnostic procedures.

Recommended Products
Alternative Products Compatible Secondaries Isotype Control Writers/Readers/Erasers Related Products
  • View More Images（6）
    $ 420
    Anti-Propionyl-Histone H3 (Lys23) Mouse mAb
    Catalog #
    PTM-208
    Host
    Mouse
    Clonality
    Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Mouse, Rat
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
  • View More Images（4）
    $ 135
    Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
    Catalog #
    PTM-6261
    Host
    Goat
    Clonality
    Polyclonal
    Conjugate
    HRP
    Reactivity
    Rabbit
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
  • View More Images（5）
    $ 420
    Recombinant Rabbit Monoclonal
    Anti-2-Hydroxyisobutyryl-Histone H3 (Lys23) Rabbit mAb
    Catalog #
    PTM-843RM
    Host
    Rabbit
    Clonality
    Recombinant Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Mouse, Rat
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
  • View More Images（2）
    $ 340
    Anti-Acetyl-Histone H3 (Lys23) Rabbit pAb
    Catalog #
    PTM-115
    Host
    Rabbit
    Clonality
    Polyclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Mouse, Rat
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
  • View More Images（4）
    $ 420
    Anti-Butyryl-Histone H3 (Lys23) Mouse mAb
    Catalog #
    PTM-307
    Host
    Mouse
    Clonality
    Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Mouse, Rat
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
  • View More Images（6）
    $ 435
    Anti-Crotonyl-Histone H3 (Lys23) Mouse mAb
    Catalog #
    PTM-519
    Host
    Mouse
    Clonality
    Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Mouse, Rat
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
  • View More Images（9）
    $ 320
    Anti-Dimethyl-Histone H3 (Lys23) Mouse mAb
    Catalog #
    PTM-676
    Host
    Mouse
    Clonality
    Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Mouse, Rat, Monkey
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
  • View More Images（5）
    $ 360
    Recombinant Rabbit Monoclonal
    Anti-Trimethyl-Histone H3 (Lys23) Rabbit mAb
    Catalog #
    PTM-638RM
    Host
    Rabbit
    Clonality
    Recombinant Monoclonal
    Conjugate
    Unconjugated
    Reactivity
    Human, Mouse, Rat
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
  • View More Images（2）
    $ 440
    Anti-Propionyllysine Rabbit pAb
    Catalog #
    PTM-201
    Host
    Rabbit
    Clonality
    Polyclonal
    Conjugate
    Unconjugated
    Reactivity
    All
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
  • View More Images（1）
    $ 2,110
    Anti-Propionyllysine Antibody Conjugated Agarose Beads
    Catalog #
    PTM-202
    Host
    /
    Clonality
    /
    Conjugate
    Agarose
    Reactivity
    All
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
References (2)
  • Deficient histone H3 propionylation by BRPF1-KAT6 complexes in neurodevelopmental disorders and cancer
    Year 2020
    Journal Science Advances
    Authors Kezhi Yan, et al.
    Applications Unspecified
    Reactivity
  • Histone acylation marks respond to metabolic perturbations and enable cellular adaptation
    Year 2020
    Journal EXPERIMENTAL AND MOLECULAR MEDICINE
    Authors Jo Chanhee, et al.
    Applications Unspecified
    Reactivity
Service
Products
Support
About Us
© 2023 PTM Biolabs Inc.