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Recombinant Rabbit Monoclonal
Anti-Propionyl-Histone H3 (Lys56) Rabbit mAb
Catalog Number: PTM-220RM
$ 435

Clone Number: PTM-012-5

Host: Rabbit Clonality: Monoclonal

Applications： WB IHC-P

Reactivity: Human, Mouse, Rat

Synonyms: H3K56pr

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms H3K56pr
UniProt ID

P68431

Immunogen
MW (kDa) 15
Specificity
Product Usage Information
Applications Dilution Recommended Species
WB 1:500-1:1000 Human, Mouse, Rat
IHC-P 1:100-1:500 Human, Mouse, Rat
IP 1:50-1:100 Human
Properties
Purity Protein A purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. Lysine propionylation (Kprop) is structurally similar to lysine acetylation, is a newly identified reversible modification controlling protein activity. Lysine propionylation is abundant in both prokaryotes and eukaryotes and has been found in wide ranges of proteins including histones and non-histone substrates, such as p53. Similar to acetylation of histone H4 at Lys5, propionylation of histone H4 at Lys5 may play a vital role in the epigenetic modulation, including chromatin remodeling and transcriptional regulation.

Cellular location

Nucleus

Images
Dot Blot

Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:5000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Rabbit IgG H&L (HRP)
Immunogen peptide quantity: 1 ng, 4 ng, 16 ng, 64ng
Exposure time: 30 s
The list of peptides is included in the table below:
WB

Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:1000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: (-) HeLa, (+) HeLa+sodium butyrate (30mM, 4 h)
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 15 kDa
Observed MW: 15 kDa

Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:1000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: 1: HeLa, 2: NIH-3T3, 3: BRL, 4: Rat kidney, 5: Mouse kidney
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 15 kDa
Observed MW: 15 kDa
IHC-P

Tissue: Human prostate
Section type: Formalin fixed & Paraffin – embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary ab dilution: 1:500
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for PTM-220RM

Tissue: Mouse colon
Section type: Formalin fixed & Paraffin – embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary ab dilution: 1:500
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for PTM-220RM

Tissue: Rat colon
Section type: Formalin fixed & Paraffin – embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary ab dilution: 1:500
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for PTM-220RM
IP

IP of HeLa+ sodium butyrate (30mM, 4 h) cells extracts
IP ab incubation condition: PTM-220RM, 4°C overnight, 1:100 dilution
WB primary ab incubation condition: PTM-220RM, room temperature 2h, 1:1000 dilution
Secondary ab: Anti-Rabbit IgG for IP (HRP)
Blocking buffer and concentration: 5%
NFDM/TBST
Diluting buffer and concentration: 5%
NFDM/TBST
1: 5% Input
2: IP with PTM-220RM
3: IP with Rabbit monoclonal IgG Isotype
Predicted MW: 15 kDa
Observed MW: 15 kDa
Exposure time: 30 s

Research Use

For research use only, not for use in diagnostic procedures.

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