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Anti-Propionyl-Histone H4 (Lys16) Mouse mAb
Catalog Number: PTM-210
$ 420

Clone Number: /

Host: Mouse Clonality: Monoclonal

Applications： WB IHC-P ChIP

Reactivity: Human, Mouse, Rat

Synonyms: H4K16pr

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms H4K16pr
UniProt ID

P62805

Immunogen Propionylated human histone H4 (Lys16) peptide
MW (kDa) 11
Specificity Anti-Propionyl-Histone H4 (Lys16) Mouse mAb detects histone H4 only when it is propionylated at Lys16. This antibody has been shown to selectively recognize acetylated H4 peptide at Lys16, but not the structurally similar acetylated, butyrylated, crotonylated peptide at Lys16 or unmodified peptide.
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:2000 Human, Mouse, Rat
IHC-P 1:50 - 1:200 Human, Mouse, Rat
ChIP 6 μg per 5x106 cells Human
Properties
Purity Protein G and immunogen affinity purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation,phosphorylation, ubiquitylation, etc. Lysine propionylation (Kpr) is a newly identified reversible modification structurally similar to lysine acetylation and can control protein activity. Lysine propionylation is abundant in both prokaryotes and eukaryotes and has been found in wide ranges of proteins including histones and non-histone substrates, such as p53. Similar to acetylation of histone H3 at Lys12, propionylation of histone H4 at Lys16 may play a vital role in the epigenetic modulation, including chromatin remodeling and transcriptional regulation.

Cellular location

Nucleus

Images
Dot Blot

Peptide amount: 1ng, 4 ng, 16 ng
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)
Exposure time: 60 seconds
The list of peptides used in the experiment is provided below.
Lane 1: H4K16 propionyl. Lane 2: H4K12 acetyl.
Lane 3: H4K12 propionyl. Lane 4: H4K12 butyryl.
Lane 5: H4K16 acetyl. Lane 6: H4K16 butyryl.
Lane 7: H4K16 crotonyl. Lane 8: H4K20 monomethyl.
Lane 9: H4K20 dimethyl. Lane 10: H4K16 unmodified.
WB

Lysates: (-): HeLa cells; (+): HeLa cells treated with 10 mM sodium propionate for 4 hours
Protein loading amount: 20 μg
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)
Exposure time: 60 seconds
Predicted band size: 11 kDa
Observed band size: 11 kDa
IHC-P

Tissue: human spleen
Section type: formalinfixed & paraffin-embedded section
Retrieval method: high temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:200
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: hematoxylin (blue)
Description: The brown color represents the positive signal observed with PTM-210.

Tissue: mouse hippocampus
Section type: formalinfixed & paraffin-embedded section
Retrieval method: high temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:200
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: hematoxylin (blue)
Description: The brown color represents the positive signal observed with PTM-210.

Tissue: rat spleen
Section type: formalinfixed & paraffin-embedded section
Retrieval method: high temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:200
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: hematoxylin (blue)
Description: The brown color represents the positive signal observed with PTM-210.
ChIP

Sample: HeLa cells treated with 10 mM sodium propionate for 4 hours
Cross-linking conditions: no cross-linking
Amount of chromatin per IP: 5×106 cells
Amount of Ab per IP: 6 μg
Beads type and amount per IP: 50 μL of Protein G MagBeads
Description: Chromatin immunoprecipitations were performed with 6 μg of normal mouse IgG as a negative control. The immunoprecipitated DNA was quantified by real-time PCR using primers specific for the human GAPDH CDS, RPL30, LDHA promoter, LDHA CDS, FOXO3a promoter, FOXO3a downstream, RAB20, and TUBBP10 regions. The data are presented as enrichment of each sample relative to total amount of input chromatin at each amplicon.

Research Use

For research use only, not for use in diagnostic procedures.

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    $ 160
    Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)
    Catalog #
    PTM-7168
    Host
    Goat
    Clonality
    Polyclonal
    Conjugate
    HRP
    Reactivity
    Mouse
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
References (1)
  • Lysine acetyltransferase 8 is involved in cerebral development and syndromic intellectual disability
    Year 2020
    Journal JOURNAL OF CLINICAL INVESTIGATION
    Authors Lin Li, et al.
    Applications Unspecified
    Reactivity
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