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Anti-Propionyl-Histone H4 (Lys8) Mouse mAb
Catalog Number: PTM-218
$ 420

Clone Number: /

Host: Mouse Clonality: Monoclonal

Applications： WB IHC-P ChIP

Reactivity: Human, Mouse, Rat

Synonyms: H4K8pr

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms H4K8pr
UniProt ID

P62805

Immunogen
MW (kDa) 11
Specificity
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:1000 Human, Mouse, Rat
IHC-P 1:200 - 1:1000 Human, Mouse, Rat
ChIP 4 μg/5x106 cells Human
Properties
Purity Protein G and immunogen affinity purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. Lysine propionylation (Kprop) is structurally similar to lysine acetylation, is a newly identified reversible modification controlling protein activity. Lysine propionylation is abundant in both prokaryotes and eukaryotes and has been found in wide ranges of proteins including histones and non-histone substrates, such as p53. Similar to acetylation of histone H3 at Lys12, propionylation of histone H4 at Lys8 may play a vital role in the epigenetic modulation, including chromatin remodeling and transcriptional regulation.

Cellular location

Nucleus

Images
Dot Blot

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)
Immunogen peptide quantity: 1 ng, 4 ng, 16 ng
Exposure time: 60 s
The list of peptides is included in the table below.
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)
Lysate: (-) HeLa, (+) Hela + sodium butyrate (30mM, 4 hours)+ Trichostatin A (500ng/ml, 4 hours)
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted band size: 11 kDa
Observed band size: 11 kDa

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)
Lysate: (-) C2C12, (+) C2C12+Sodium butyrate (30mM, 4 hours)
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted band size: 11 kDa
Observed band size: 11 kDa
IHC-P

Tissue: Human spleen
Section type: Formalin fixed & Paraffin – embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:200
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (Blue)
Description: The brown color represents the positive signal observed with PTM-218

Tissue: Mouse testis
Section type: Formalin fixed & Paraffin – embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (Blue)
Description: The brown color represents the positive signal observed with PTM-218

Tissue:Rat spleen
Section type: Formalin fixed & Paraffin – embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:200
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (Blue)
Description: The brown color represents the positive signal observed with PTM-218
ChIP

Cell type: HeLa+ Sodium butyrate (30 mM, 4 hours)+ Trichostatin A (500 ng/ml, 4 hours)
Cross-linking conditions: no cross-linking
Amount of chromatin per IP: 5x106 cells
Amount of Ab per IP: 4 ug
Beads type and amount per IP: 50 μl of Protein A/G MagBeads
Description: Chromatin immunoprecipitations were performed with 6 μg of normal mouse IgG as a negative control. The immunoprecipitated DNA was quantified by real-time PCR using primers specific for the human GAPDH CDS region, RPL30 Exon 3, LDHA, FOXO3a-promoter, FOXO3a-downstream, RAB20 and TUBBP10. The data are presented as enrichment of each sample relative to total amount of input chromatin at each amplicon.

Research Use

For research use only, not for use in diagnostic procedures.

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    $ 160
    Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)
    Catalog #
    PTM-7168
    Host
    Goat
    Clonality
    Polyclonal
    Conjugate
    HRP
    Reactivity
    Mouse
    Applications：
    WB IHC-P ICC/IF FC IAP IP ChIP mIHC-P
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