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Recombinant Rabbit Monoclonal
Anti-Propionyllysine Mouse mAb
Catalog Number: PTM-203
$ 420

Clone Number: 13E5

Host: Mouse Clonality: Monoclonal

Applications： WB IHC-P ChIP

Reactivity: All

Synonyms: Kpr

Product Size
100μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms Kpr
UniProt ID

/

Immunogen Propionylated lysine peptides
MW (kDa) Multiple
Specificity Anti- Propionyllysine Mouse mAb detects proteins with propionylated lysine residues. This pan antibody recognizes propionylated lysine independent of its surrounding sequences.
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:1000 All
IHC-P 1:200 - 1:1000 Human
ChIP 6 μg per 5x106 cells Human
Properties
Purity Protein G and immunogen affinity purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Propionylation of lysine, structurally similar to lysine acetylation, is a newly identified reversible modification controlling protein activity. The reversible lysine propionylation has been well demonstrated in both prokaryotes and eukaryotes in wide ranges of proteins including histones and non-histone substrates, such as p53. It is speculated that lysine propionylation plays a vital role in the regulation of multiple cellular processes including chromatin dynamics, plasticity, and DNA transcriptional regulation by sharing same regulative enzymes with lysine acetylation or with its unique regulative enzymes.

Cellular location

/

Images
Mass Spectrometry Analysis

Mass spectrometer analysis: Representative annotated MS/MS spectra of a propionyllysine remnant-containing peptide. Crude proteins from mouse liver were digested by trypsin. The propionyllysine remnant-containing peptides were affinity enriched by the Anti-Propionyllysinemouse mAb (PTM-203) followed by LC-ESI-MS/MS in a Q-Exactive mass spectrometer. The sequence of the representative propionylated peptide is indicated and the fragment ions are labeled in the MS/MS spectrum.
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)
Lysate: (-) MCF-7; (+) MCF-7 + sodium butyrate (30 mM, 4 hours) + Trichostatin A (500 ng/ml, 24 hours)
Protein loading amount: 20 μg
Exposure time: 120 s
Predicted band size: Multiple
Observed band size: Multiple

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)
Lysate: (-) HeLa; (+) HeLa + sodium butyrate (30 mM, 4 hours)
Protein loading amount: 20 μg
Exposure time: 120 s
Predicted band size: Multiple
Observed band size: Multiple
IHC-P

Tissue: Human kidney
Section type: Formalin-fixed & paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (Blue)
Description: The brown color represents the positive signal observed with PTM-203.

Tissue: Human placenta
Section type: Formalin-fixed & paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (Blue)
Description: The brown color represents the positive signal observed with PTM-203.
ChIP

Cell type: HeLa + sodium butyrate (5 mM, 24 hours)
Cross-linking conditions: No cross-linking
Amount of chromatin per IP: 5x106 cells
Amount of ab per IP: 6ug
Beads type and amount per IP: 50 μl of Protein A/G MagBeads
Description: Chromatin immunoprecipitations were performed with 6 μg of normal mouse IgG as a negative control. The immunoprecipitated DNA was quantified by real-time PCR using primers specific for the human GAPDH CDS and LDHA CDS regions. Data is presented as enrichment of each sample relative to total amount of input chromatin at each amplicon.

Research Use

For research use only, not for use in diagnostic procedures.

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