中 / EN
Back
QUICK ORDER
Technical Support
| Contact Us
+1 312-802-6843
Technical Support
Contact Us
+1 312-802-6843
中 / EN
QUICK ORDER
Proteomics Bioinformation Research Antibody Products IVD Products Custom Antibody Development
Recover password
Professional Email
Verification Code
Send Code
Password
Recombinant
Anti-PSMD9 Mouse mAb
Catalog Number: PTM-5864
$ 290

Clone Number: 10678

Host: Mouse Clonality: Recombinant Monoclonal

Applications： WB IHC-P ICC/IF

Reactivity: Human, Mouse, Rat

Synonyms: 26S proteasome non-ATPase regulatory subunit 9, 26S proteasome regulatory subunit p27

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG1
Conjugate Unconjugated
Synonyms 26S proteasome non-ATPase regulatory subunit 9, 26S proteasome regulatory subunit p27
UniProt ID

O00233

Immunogen Synthetic peptide derived from human PSMD9
MW (kDa) 30
Specificity
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:1000 Human, Mouse
IHC-P 1:50 - 1:100 Human, Mouse, Rat
ICC/IF 1:50 Human
Properties
Purity Protein G purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Acts as a chaperone during the assembly of the 26S proteasome, specifically of the base subcomplex of the PA700/19S regulatory complex (RC). During the base subcomplex assembly is part of an intermediate PSMD9: PSMC6:PSMC3 module, also known as modulator trimer complex; PSMD9 is released during the further base assembly process.

Cellular location

Cytosol, Nucleus

Images
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation: 2 hours at room temperature
Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)
Lysate: 3T3, HepG2, A549
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted band size: 30 kDa
Observed band size: 30 kDa
IHC-P

Tissue: Human cervical carcinoma
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:100
Primary Ab incubation: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (Blue)
Description: The brown color represents the positive signal observed with PTM-5864

Tissue: Mouse kidney
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:100
Primary Ab incubation: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (Blue)
Description: The brown color represents the positive signal observed with PTM-5864

Tissue: Rat liver
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:100
Primary Ab incubation: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (Blue)
Description: The brown color represents the positive signal observed with PTM-5864
ICC/IF

Cell line: HeLa
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% TritonX-100
Primary Ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary Ab: Goat Anti-Mouse IgG
Nuclear counter stain: DAPI (Blue)
Description: The green color represents the positive signal observed with PTM-5864

Research Use

For research use only, not for use in diagnostic procedures.

Service
Products
Support
About Us
© 2025 PTM BIO LLC, or its affiliates