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Anti-RING1B Rabbit pAb
Catalog Number: PTM-7009
$ 290

Clone Number: /

Host: Rabbit Clonality: Polyclonal

Applications: WB

Reactivity: Human

Synonyms: RNF2, DING, HIPI3, RING2

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms RNF2, DING, HIPI3, RING2
UniProt ID

Q99496

Immunogen
MW (kDa) 38
Specificity
Product Usage Information
Applications Dilution Recommended Species
WB 1:1000 - 1:2000 Human
Properties
Purity Protein A and immunogen affinity purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

RING2 is an E3 ubiquitin-protein ligase that mediates monoubiquitination of 'Lys-119' of histone H2A, thereby playing a central role in histone code and gene regulation. H2A 'Lys-119' ubiquitination gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. RING2 is an essential component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility. E3 ubiquitin-protein ligase activity is enhanced by BMI1/PCGF4. RING2 acts as the main E3 ubiquitin ligase on histone H2A of the PRC1 complex, while RING1 may rather act as a modulator of RNF2/RING2 activity.

Cellular location

Cytoplasm

Images
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)
Lysate: 1: 293T, 2: Jurkat
Protein loading amount: 20 μg
Exposure time: 60 s
Predicted band size: 38 kDa
Observed band size: 38 kDa

Research Use

For research use only, not for use in diagnostic procedures.