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Recombinant
Anti-RPL3 Mouse mAb
Catalog Number: PTM-5394
$ 280

Clone Number: 10186

Host: Mouse Clonality: Recombinant Monoclonal

Applications: WB IHC-P ICC/IF

Reactivity: Human, Mouse

Synonyms: TARBP-B

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG1/kappa
Conjugate Unconjugated
Synonyms TARBP-B
UniProt ID

P39023

Immunogen
MW (kDa)
Specificity
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:1000 Human, Mouse
IHC-P 1:50 - 1:100 Human
ICC/IF 1:50 Human
Properties
Purity Protein A purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

RPL3, also named as TARBP-B and MGC104284, belongs to the ribosomal protein L3P family. This protein is a component of the 60S subunit. It can bind to the HIV-1 TAR mRNA, and it has been suggested that the protein contributes to tat-mediated transactivation. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

Cellular location

Cytoplasm, Nucleus

Images
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 2 hoursat room temperature
Secondary Ab: Goat Anti-Mouse IgGH&L(HRP Conjugate)
Lysate: 1: HepG2, 2: NIH/3T3, 3: HeLa, 4: HEK-293T
Protein loading quantity: 20 μg
Exposure time: 60 seconds
Predicted band size: 46 kDa
Observed band size: 55 kDa

IHC-P

Tissue: Human liver cancer
Section type: Formalin-fixed & Paraffin-embedded section
Retrieval method: High temperature and highpressure
Retrieval buffer: Tris/EDTA buffer, pH9.0
Primary Ab dilution: 1:100
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)
Counter stain: Hematoxylin (Blue)
Description: The brown color represents the positive signal observed with PTM-5394

ICC/IF

Cell line: HepG2
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% Triton X-100
Primary Ab dilution: 1:50
Primary Ab incubation condition: 4°C overnight
Secondary Ab: Goat Anti-Mouse IgG
Nuclear counter stain: DAPI (Blue)
Description: The green color represents the positive signal observed with PTM-5394

Research Use

For research use only, not for use in diagnostic procedures.