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Recombinant
Anti-SDHA Mouse mAb
Catalog Number: PTM-5162
$ 280

Clone Number: 12396

Host: Mouse Clonality: Recombinant Monoclonal

Applications: WB IHC-P

Reactivity: Human, Mouse, Rat

Synonyms: SDH2, SDHF

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG1
Conjugate Unconjugated
Synonyms SDH2, SDHF
UniProt ID

P31040

Immunogen
MW (kDa)
Specificity
Product Usage Information
Applications Dilution Recommended Species
WB 1:1000 Human, Mouse, Rat
IHC-P 1:50 - 1:100 Human, Mouse
Properties
Purity Protein G purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). Can act as a tumor suppressor.

Cellular location

Membrane, Mitochondrion, Mitochondrion inner membrane

Images
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation: 2 hours at room temperature
Lysate: HeLa, HepG2, Neuro-2a, BRL, EL4.IL-2
Protein loading quantity: 20 μg
Exposure time: 10 seconds
Predicted band size: 70 kDa
Observed band size: 70 kDa

IHC-P

Tissue: Human liver
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:100
Primary Ab incubation: 1 hour at room temperature
Counter stain: Hematoxylin
Description: The brown color represents the positive signal observed with PTM-5162

Tissue: Human colon cancer
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:100
Primary incubation condition: 1 hour at room temperature
Counter stain: Hematoxylin
Description: The brown color represents the positive signal observed with PTM-5162

Tissue: Mouse myocardium
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:100
Primary incubation condition: 1 hour at room temperature
Counter stain: Hematoxylin
Description: The brown color represents the positive signal observed with PTM-5162

Research Use

For research use only, not for use in diagnostic procedures.

References (1)
  • Mitochondrial and glucose metabolic dysfunctions in granulosa cells induce impaired oocytes of polycystic ovary syndrome through Sirtuin 3
    Year 2022
    Journal FREE RADICAL BIOLOGY AND MEDICINE
    Authors Qing Zhang, et al.
    Applications Unspecified
    Reactivity