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Recombinant Rabbit Monoclonal
Anti-SIN3A Rabbit mAb
Catalog Number: PTM-7458
$ 280

Clone Number: JRMR-N1211

Host: Rabbit Clonality: Recombinant Monoclonal

Applications： WB IP

Reactivity: Human

Synonyms: /

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms /
UniProt ID

Q96ST3

Immunogen
MW (kDa) 150
Specificity
Product Usage Information
Applications Dilution Recommended Species
WB 1:1000 - 1:2000 Human
IHC-P 1:100 - 1:1000 Human, Mouse, Rat
IP 1:50 - 1:100 Human
Properties
Purity Protein A purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

on Background Acts as a transcriptional repressor. Corepressor for REST. Interacts with MXI1 to repress MYC responsive genes and antagonize MYC oncogenic activities. Also interacts with MXD1-MAX heterodimers to repress transcription by tethering SIN3A to DNA. Acts cooperatively with OGT to repress transcription in parallel with histone deacetylation. Involved in the control of the circadian rhythms. Required for the transcriptional repression of circadian target genes, such as PER1, mediated by the large PER complex through histone deacetylation. Cooperates with FOXK1 to regulate cell cycle progression probably by repressing cell cycle inhibitor genes expression. Required for cortical neuron differentiation and callosal axon elongation.

Cellular location

Nucleus FOR RESEARCH USE ONLY. NOT FOR USE

Images
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: 1: K562, 2: HeLa, 3: MCF-7, 4: Jurkat
Protein loading amount: 20 μg
Exposure time: 60 s
Predicted MW: 145 kDa
Observed MW: 150 kDa
IHC-P

Tissue: Human colon
Section type: Formalin-fixed & paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use)
Counter stain: Hematoxylin (blue)
Comment: Color brown is the positive signal for PTM-7458

Tissue: Mouse cerebrum
Section type: Formalin-fixed & paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use)
Counter stain: Hematoxylin (blue)
Comment: Color brown is the positive signal for PTM-7458

Tissue: Rat cerebrum
Section type: Formalin-fixed & paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use)
Counter stain: Hematoxylin (blue)
Comment: Color brown is the positive signal for PTM-7458
IP

IP of K562 cells extracts:
IP Ab incubation: PTM-7458, 4°C overnight, 1:100 dilution
WB Primary Ab incubation: PTM-7458, room temperature 2h, 1:1000 dilution
Secondary Ab: Anti-Rabbit IgG for IP (HRP)
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
1: 5%% Input
2: IP with Rabbit monoclonal IgG Isotype
3: IP with PTM-7458
Observed MW: 150 kDa
Exposure time: 60 s

Research Use

For research use only, not for use in diagnostic procedures.

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