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Recombinant Rabbit Monoclonal
Anti-SIRT6 Rabbit mAb
Catalog Number: PTM-6658
$ 280

Clone Number: JRMR-10482

Host: Rabbit Clonality: Recombinant Monoclonal

Applications: WB

Reactivity: Human, Mouse, Rat

Synonyms: SIR2L6

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms SIR2L6
UniProt ID

Q8N6T7

Immunogen
MW (kDa)
Specificity
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:1000 Human, Mouse, Rat
Properties
Purity Protein A purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

SirT6, a mammalian homolog of Sir2, is a nuclear, chromatin-associated protein that promotes the normal maintenance of genome integrity mediated by the base excision repair (BER) pathway. The BER pathway repairs single-stranded DNA lesions that arise spontaneously from endogenous alkylation, oxidation, and deamination events. SirT6 deficient mice show increased sensitivity to DNA-damaging agents, including the alkylating agents MMS and H2O2. In addition, these mice show genome instability with increased frequency of fragmented chromosomes, detached centromeres, and gaps. SirT6 may regulate the BER pathway by deacetylating DNA Polβ or other core components of the pathway.

Cellular location

Nucleus, Endoplasmic reticulum

Images
WB

Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation: 2 hoursat room temperature
Secondary Ab: Goat Anti-Rabbit IgGH&L(HRP Conjugate)
Lysate: 1: HCT-116, 2: HeLa, 3: Jurkat, 4: C2C12, 5: PC-12
Protein loading quantity: 20 μg
Exposure time: 60 seconds
Predicted band size: 39 kDa
Observed band size: 42 kDa

Research Use

For research use only, not for use in diagnostic procedures.